Browse > Article
http://dx.doi.org/10.5423/PPJ.2005.21.3.229

Characterization of Trichoderma spp. Associated with Green Mold of Oyster Mushroom by PCR-RFLP and Sequence Analysis of ITS Regions of rDNA  

Park, Myung-Soo (Department of Applied Biology, Chungnam National University)
Seo, Geon-Sik (Department of Industrial Crops, Korea National Agricultural College)
Bae, Kyung-Sook (Korea Research Institute of Bioscience and Biotechnology)
Yu, Seung-Hun (Department of Applied Biology, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.21, no.3, 2005 , pp. 229-236 More about this Journal
Abstract
Molecular profIles of PCR-RFLP and sequence analysis of internal transcribed spacer (ITS) regions of rDNA were compared between morphologically distinguishable species of Trichoderma isolated from substrates of oyster mushroom in Korea, T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, Trichoderma sp. 1 and 2. PCR­RFLP analysis divided the Trichoderma spp. into six RFLP groups, A, B, C, D, E, and F. The RFLP groups were generally agreed with described morphological species, except that the RFLP group A containing the two unidentified species. A neighbor-joining tree based on ITS sequences well supported RFLP groups observed by RFLP analysis of ITS regions of rDNA. Additionally, the two unidentified species, Trichoderma sp. 1 and 2, which could not be distinguished by PCR­RFLP analysis, were separated in sequence analysis of ITS regions of rDNA.
Keywords
ITS regions of rDNA; oyster mushroom; PCR­RFLP; Trichoderma;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bissett, J. 1984. A revision of the genus Trichoderma. I. Section Longibrachiatum sect. nov. Can. J Bot. 62:924-931   DOI
2 Bissett, J. 1991c. A revision of the genus Trichoderma. IV. Additional notes on section Longibrachiatum. Can. J. Bot. 69:2418-2420   DOI
3 Kuhls, K., Lieckfeldt, E., Samuels, G. J., Kovacs, W., Meyer, W., Petrini, O., Gams, W., Bomer T., Meyer, W. and Kubicek, C. P. 1996. Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocreajecorina. Proc. Natl. Acad. Sci. USA 93:7755-7760   DOI
4 Kullnig-Gradinger, C. M., Szakacs, G. and Kubicek, C. P. 2002. Phylogeny and evolution of the genus Trichoderma: a multigene approach. Mycol. Res. 106:757-767   DOI   ScienceOn
5 Muthumeenakshi, S., Mills, P. R., Brown, A. E. and Seaby, D. A., 1994. Intraspecific molecular variation among Trichoderma harzianum isolates colonising mushroom compost in the British Isles. Microbiology 140:769-777   DOI   ScienceOn
6 Ospina-Giraldo, M. D., Royse, D. M., Thon, J. R., Chen, X. and Romaine, C. P. 1998. Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90:76-81   DOI   ScienceOn
7 Schlick, A., Kuhls, K., Meyer, W., Lieckfeldt, E., Borner, T. and Messner, K. 1994. Fingerprinting reveals gamma-ray induced mutations in fungal DNA: implication for identification of patent strains of Trichoderma harzianum. Curr. Genet. 26:74-78   DOI   ScienceOn
8 Stasz, T. E., Nixon, K., Harman, G. E., Weeden, N. F. and Kuter, G. A. 1989. Evalution of phenetic species and phylogenetic relationships in the genus Trichoderma by cladistic analysis of isozyme polymorphism. Mycologia 81:391-403   DOI
9 Thompson, J. D., Gibson, T. J., Plewniak, E., Jeanmougin, F. and Higgins, D. G. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24:4876-4882
10 Kuhls, K., Lieckfeldt, E., Samuels, G. J., Borner, T., Meyer, W. and Kubicek, C. P. 1997. Revision of Trichoderma sect. Longibrachiatum including related teleomorphs based on analysis of ribosomal DNA internal transcribed spacer sequences. Mycologia 89:442-460   DOI   ScienceOn
11 Park, M. S., Seo, G. S., Lee, K. H., Bae, K. S. and Yu, S. H. 2005. Morphological and cultural characteristics of Trichoderma spp. associated with green mold of oyster mushroom in Korea. Plant Pathol. J. 21:221-228   DOI   ScienceOn
12 Fujimori, F. and Okuda, T. 1994. Application of the random amplified polymorphic DNA using the polymerase chain reaction for efficient elimination of duplicate strains in microbial screening I Fungi. J. Antibiot. 47: 173-182   DOI   ScienceOn
13 Zimand, G, Valisky, L., Elad, Y, Chet, I. and Manulis, S. 1994. Use of the RAPD procedure for the identification of Trichoderma strains. Mycol. Res. 98:531-534   DOI   ScienceOn
14 Turner, D., Kovacs, W., Kuhls, K., Lieckfeldt, E., Peter, B., Arisan-Atac, I., Strauss, J., Samuels, G. J., Borner, T. and Kubicek, C. P. 1997. Biogeography and phenotypic variation in Trichoderma sect. Longibrachiatum and associated Hypocrea species. Mycol. Res. 101:449-459   DOI   ScienceOn
15 Bissett, J. 1991b. A revision of the genus Trichoderma. III. Section Pachybasium. Can. J. Bot. 69:2373-2417   DOI
16 Kuhls, K., Lieckfeldt, E. and Borner, T. 1995. PCR-fingerprinting used for comparison of ex-type strains of Trichoderma species deposited in different culture collections. Microbiol. Res. 150:1-9   DOI   ScienceOn
17 Bissett, J. 1991a. A revision of the genus Trichoderma. II. Infrageneric classification. Can. J Bot. 69:2357-2372   DOI
18 Kindermann, J., EI-Ayouti, Y., Samuels, G. J. and Kubicek, C. P. 1998. Phylogeny of the genus Trichoderma based on sequence analysis of the internal transcribed spacer region I of the rDNA cluster. Fungal Genet. Biol. 24:298-309   DOI   ScienceOn
19 White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A guide to methods and application (ed. M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White), pp. 315-322. Academic Press: San Diego, U.S.A
20 Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph.D. thesis, University of Newcastle, Newcastle upon Tyne, UK
21 Rifail, M. A. 1969. A revision of the genus Trichoderma. Mycological Papers 116:1-116
22 Bulat, S. A., Lubeck, M., Mironenko, N., Jensen, D. F. and Lubeck, P. S. 1998. UP-PCR analysis and ITS1 ribotyping of strains of Trichoderma and Gliocladium. Mycol. Res. 102:933-943   DOI   ScienceOn
23 Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791   DOI   ScienceOn
24 Samuels, G. J., Dodd, S. L., Gams, W., Castleburry, L. A. and Petrini, O. 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94: 146-170   DOI   ScienceOn
25 Gams, W. and Meyer, W. 1998. What exactly is Trichoderma harzianum? Mycologia 90:904-915   DOI   ScienceOn
26 Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16:111-120   DOI   PUBMED
27 Vauterin, L. and Vauterin, P. 1992. Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur. Microbiol. 1:37-41
28 Samuels, G. J., Petrini, O. and Manguin, S. 1994. Morphological and macromolecular charaterization of Hypocrea schweinitzii and its Trichoderma anamorph. Mycologia 86:421-435   DOI