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http://dx.doi.org/10.5423/PPJ.2005.21.2.142

Biological Characterization and Sequence Analysis of Cucumber mosaic virus isolated from Capsicum annuum  

Kim, Min-Jea (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University)
Choi, Seung-Kook (USDA-ARS, Department of Plant Pathology, Cornell University)
Yoon, Ju-Yeon (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University)
Choi, Jang-Kyung (Division of Biological Environment, Kangwon National University)
Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University)
Publication Information
The Plant Pathology Journal / v.21, no.2, 2005 , pp. 142-148 More about this Journal
Abstract
Whereas most of isolates of Cucumber mosaic virus(CMV) can induce green mosaic systemic symptoms on zucchini squash, foliar symptoms of a pepper isolate of CMV (Pf-CMV)-infected zucchini squash revealed systemic chlorotic spots. To assess this biological property, infectious full-length cDNA clones of Pf-CMV were constructed using long-template RT-PCR. The complete nucleotide sequences of RNA2 and RNA3 of Pf-CMV were determined from the infectious fulllength cDNA clones, respectively. RNA 2 and RNA3 of Pf-CMV contain 3,070 nucleotides and 2,213 nucleotides, respectively. Overall sequence homology of two RNAs revealed high similarity (90%) between CMV strains, and 60% similarity to those of Tomato aspermy virus and Peanut stunt virus strains. By sequence analysis with known representative strains of CMV, Pf- CMV belongs to a typical member of CMV subgroup IA. The virus has high evolutionary relationship with Fny-CMV, but the pathology of Pf-CMV in zucchini squash was quite different from that of Fny-CMV. The pesudorecombinant virus, F1P2P3, induced chlorotic spot leaf symptom and timing of systemic symptom in squash plants, similar to the plants infected by Pf-CMV. No systemic symptoms were observed when Pf-CMVinoculated cotyledons were removed at 5 days postinoculation (dpi) while Fny-CMV showed systemic symptom at 2 dpi. These results suggest that the pepper isolate of CMV possesses unique pathological properties distinguishable to other isolates of CMVs in zucchini squash.
Keywords
Cucumber mosaic virus; Cucumovirus; evolution; pepper; sequence analysis; symptom; virus movement;
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1 Canto, T. and Palukaitis, P. 1998. Transgenically expressed cucumber mosaic virus RNA 1 simultaneously components replication of cucumber mosaic virus RNA2 and 3 and confers resistance to systemic infection. Virology 250:325-336   DOI   ScienceOn
2 Choi, S. K., Ahn, H. I., Kim, M. J., Choi, J. K. and Ryu, K. H. 2004. Symptom determinant as RNA3 of lily isolates of Cucumber mosaic virus on zucchini squash. Plant Pathol. J. 20:212-219   과학기술학회마을   DOI   ScienceOn
3 Deom, C. M., Quan, S. and He, X. Z. 1997. Replicase proteins as determinants of phloem-dependent long-distance movement of tobamoviruses in tobacco. Protoplasma 19: 1-8   DOI
4 Ding, S. W., Li, W. X. and Symons, R. H. 1995. A Noble naturally occuring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J. 14:5762-5772
5 Kaplan, I. B., Gal-On, A. and Palukaitis, P. 1997. Characterization of cucumber mosaic virus. III. Localization of sequence in the movement protein controlling systemic infection in cucurbits. Virology 230:343-349   DOI   ScienceOn
6 Rizzo, T. M. and Palukaitis, P. 1990. Construction of full-length cDNA clones of cucumber mosaic virus RNAs 1, 2, and 3: generation of infectious RNA transcripts. Mol. Gen. Genet. 222:249-256   DOI
7 Salanki, K., Balazs, E. and Burguan, J. 1994. Nucloetide sequence and infectious in vivo transcripts of RNA3 of tomato aspermy virus pepper isolate. Virus Res. 33:281-289   DOI   ScienceOn
8 Smirnyagina, E., Hsu, Y. H., Chua, N. and Ahlquist, P. 1994. Second-site mutations in the brome mosaic virus RNA3 intercis-tronic region partially suppress a defect in coat protein mRNA transcription. Virology 198:427-436   DOI   ScienceOn
9 Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York
10 Szilassy, D., Salanki, K. and Balzas, E. 1999. Molecular evidence for the existence of two distinct subgroups in cucumber mosaic virus Virus Genes 18:221-227   DOI   ScienceOn
11 Kim, C. H. and Palukaitis, P. 1997. The plant defense response to cucumber mosaic virus in cowpea is elicited by viral polymerase gene and affects virus accumulation in single cells. EMBO J. 16:4060-4068   DOI   ScienceOn
12 Sanger, F., Nicklen, S. and Coulson, A. R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467
13 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41 :281-348   DOI
14 Palukaitis, P. and Garcia-Arenal, F. 2003. Cucumoviruses. Adv. Virus Res. 62:241-323   DOI
15 Ding, S. W., Anderson, B. J., Haase, H. R. and Symons, R. H. 1994. New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593-601   DOI   ScienceOn
16 Takeshita, M., Suzuki, M., Kuwata, S. and Takanami, Y. 1998. Involvement of cucumber mosaic cucumovirus RNA 2 and RNA 3 in viral systemic spread in radish plant. Arch. Virol. 143:1109-1117   DOI   ScienceOn
17 Lucy, L. P., Guo, H. H., Li, X. W. and Ding, S. W. 2000. Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J. 9: 1672-1680
18 Moriones, E., Roossinck, M. J. and Garcia-Arenal, F. 1991. Nucleotide sequence of tomato aspermy virus RNA 2. J. Gen. Virol. 72:779-783   DOI   ScienceOn
19 McGarvey, P., Tousignant, M., Geletka, L., Cellini, F. and Kaper, J. M. 1995. The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs. J. Gen. Virol. 76:2257-2270   DOI   ScienceOn
20 Shin, R., Han, J. H., Lee, G. J. and Paek, K. H. 2002a. The potential use of a viral coat protein gene as a transgene screening marker and multiple virus resistance of pepper plants coexpressing coat proteins of cucumber mosaic virus and tomato mosaic virus. Transgenic Res. 11:215-219   DOI   ScienceOn
21 Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangements in the 5' nontranslated region and phylogenetic analyses of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J. Virol. 73:6752-6758
22 Rezaian, M. A., Williams, R. H. V. and Symons, R. H. 1985. Nucleotide sequence of cucumber mosaic virus RNA 1. Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNAs. Eur. J. Biochem. 150:331-339   DOI   ScienceOn
23 Canto, T., Prior, D. A, Hellwald, K. H., Oparka, K. J. and Palukaitis, P. 1997. Characterization of cucumber mosaic virus. IV. Movement protein and coat protein are both essential for cell-to-cell movement of cucumber mosaic virus. Virology 237:237-248   DOI   PUBMED   ScienceOn
24 Nelson, R. S. and van Bel, A. J. E. 1998. The mystery of virus trafficking into, through and out of vascular tissue. Prog. Bot. 59:476-533   DOI
25 Peden, K. W. C. and Symons, R. H. 1979. Cucumber mosaic virus contains a functionally divided genome. Virology 53:487-492   DOI   ScienceOn
26 Chen, J., Watanabe, Y., Sako, N., Ohshima, K. and Okada, Y. 1996. Mapping of host range restriction of the Rakkyo strain of tobacco mosaic virus in Nicotiana tabacum cv. Bright Yellow. Virology 226: 198-204   DOI   ScienceOn
27 Gal-On, A., Kaplan, I. B., Roossinck, M. J. and Palukaitis, P. 1994. The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement. Virology 187:499-507   DOI   ScienceOn
28 Wong, S. M., Thio, S. S. C., Shinkaku, M. H. and Palukaitis, P. 1999. The rate of cell-to-cell movement in squash of cucumber mosaic virus is affected by sequences of the capsid protein. Mol. Plant-Microbe Interact. 12:628-632   DOI
29 Hayes, R. J. and Buck, K. W. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363-368   DOI   ScienceOn
30 Shin, R, Park, J. M., Ah, J. M. and Paek, K. H. 2002b. Ectopic expression of Tsil in transgenic hot pepper plants enhances host resistance to viral, bacterial, and oomycete pathogens. Mol. Plant Microbe Interact. 15:983-989   DOI   ScienceOn
31 Roossinck, M. J. and Palukaitis, P. 1990. Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA 1 of cucumber mosaic virus. Mol. Plant-Microbe Interact. 3: 188-192   DOI
32 Brunt, A., Crabtree, K., Dallwitz, M., Gibbs, A. and Watson, L. 1996. Viruses of Plants: Descriptions and Lists from the VIDE Database. 1484 pp. C.A.B. International, U.K
33 Ryu, K. H., Kim, C. H. and Palukaitis, P. 1998. The coat protein of cucumber mosaic virus is a host range determinant for infection of maize. Mol. Plant-Microbe Interact. 11:351-357   DOI   ScienceOn
34 Choi, S. K., Choi, J. K. and Ryu, K. H. 2003. Involvement of RNA2 for systemic infection of Cucumber mosaic virus isolated from lily on zucchini squash. Virus Res. 97: 1-6   DOI   ScienceOn
35 Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83:67-73   DOI   ScienceOn
36 Boccard, F. and Baulcombe, D. 1993. Mutational analysis of cisacting sequence and gene function in RNA3 of cucumber mosaic virus. Virology 193:563-578   DOI   ScienceOn