Browse > Article
http://dx.doi.org/10.5423/PPJ.2005.21.2.132

Two Groups of Phytoplasma from Chrysanthemum (Dendranthema grandiflorum) Distinguished by Symptoms and 16S rRNA Gene Sequence in Korea  

Chung, Bong-Nam (Horticultural Environment Division, National Horticultural Research Institute, RDA)
Kim, Byung-Dong (Department of Plant Science and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Publication Information
The Plant Pathology Journal / v.21, no.2, 2005 , pp. 132-136 More about this Journal
Abstract
Two groups of phytoplasma were identified in chrysanthemum(Dendranthema grandiflorum) cv. Chunkwang showing distinct symptoms. Isolate Ph-ch1 showed symptoms of dwarf, witches'-broom, rosette and root death. The other isolate, Ph-ch2, revealed symptoms of dwarf, yellowing, leaf cupping, vein clearing and root death. The presence of phytoplasma structures in chrysanthemum leaf tissue was confirmed by transmission electron microscopy. The 16S rRNA gene was amplified from isolates Ph-ch1 and Ph-ch2 by PCR and cloned, and the nucleotide sequences were determined. In RFLP analysis, isolate Ph-ch2 showed profiles identical to Ph-ch1, except with restriction enzymes HhaI and MseI. The sequence data showed that isolate Ph-ch1 was most closely related to the aster yellows (AY) phytoplasma, and isolate Ph-ch2 was more closely related to stolbur phytoplasma than to AY phytoplasma. This is the first reported observation of stolbur phytoplasma in chrysanthemum species.
Keywords
aster yellows; chrysanthemum; Dendranthema grandiflorum; phytoplasma; stolbur;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Appiano, A., D'Giovanni, G. and Conti, M. 1983. Ultractural observations on chrysanthemum plants infected by a mycoplasma-like organism. Giornale Botanico Italiano 117:136-137
2 Lee, I. M. Hammond, R. W., Davis, R. E. and Gundersen, D. E. 1993. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology 83:834-842   DOI   ScienceOn
3 Ministry of Agriculture & Forestry. 2002. Floriculture cultivation status for 2001. Ministry of Agriculture & Forestry p. 1-161
4 Lee, J. T. 1998. Diagnosis and transmission of phytoplasma disease. In: A Study on Plant Virololy, pp. 165-182. Pro I. Y. So, Retirement Festschrift
5 Lee, I. M., Gundersen, D. E., Hammond, R. W. and Davis, R. E. 1994. Use of mycoplasmal ike organisms (MLO) group-specific oligonucleotide primers for nested PCR assays to detect mixed MLO infections in a single host plant. Phytopathology 84:559-566   DOI   ScienceOn
6 Doi, Y., Teranaka, M., Yora, K. and Asuyama, H. 1967. Mycoplasma-or PLT-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches 'broom, aster yellows, or paulownia witches' -broom. Ann. Phytopathol. Soc. Jpn. 33:259-266   DOI
7 Bertaccini, A., Davis, R. E., Hammond, R. W., Vibio, M., Bellardi, M. G. and Lee, I. M. 1992. Sensitive detection of my coplasmal ike organisms in field collected and in vitro propagated plants of Brassica, Hydrangea and chrysanthemum by polymerase chain reaction. Ann. Appl. Biol. 121 :593-599   DOI
8 Bertaccini, A., Davis, R. E. and Lee, I. M. 1990. Detection of chrysanthemum yellows mycoplasma-like organism by dot hybridization and southern blot analysis. Plant Dis. 74:40-43   DOI
9 Kunkel, L. O. 1926. Incubation period of aster yellows in its insect host. Phytopathology 16:67 (Abstr.)
10 Chung, B. N. and Jeong M. I. 2003. Detection and molecular characterization of a Stolbur phytoplasma in Lilium oriental hybrids. Plant Pathol. J. 19: 106-110   과학기술학회마을   DOI   ScienceOn
11 Lee, I. M., Gundersen, D. E., Davis, R. E. and Bartoszyk, I. M. 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int. J. Syst. Bacteriol. 48: 1153-1169   DOI
12 McCoy, R. E., Caudwell, A, Chang, C. J., Chen, T. A., Chiykowski, L. N., Cousin, M. T., Dale, J. L., de Leeuw, G. T. N., Golino, D. A, Hackett, K. J., Kirkpatrick, B. C., Marwitz, R., Petzold, H., Sinha, R. C., Sugiura, M., Whitecomb, R. F., Yang, I. L., Zhu, B. M. and Seemuller, E. 1989. Plant diseases associated with mycoplasmalike organisms. In: The Mycoplasmas vol. 5, ed. by R. F. Whitcomb and J. G. Tully, pp. 545-640. Academic Press, New York
13 Hopkins, D.I. 1977. Diseases caused by leafhopper-borne rickettsia like bacteria. Annu. Rev. Phytopath. 17:277-294
14 Lee, I. M. and Davis, R. E. 1983. Phloem-limited prokaryotes in sieve elements isolated by enzyme treatment of diseased plant tissues. Phytopathology 73:1540-1543   DOI
15 Seemuller, E., Schneider, B., Maret, R., Ahrens, U., Daire, X., Kison, H., Lorenz, K. H., Firrao, G and Avinent, L. 1994. Phylogenetic classification of phytopathogenic Mollicutes by sequence analysis of 16S ribosomal DNA. Int. J. Syst. Bacteriol. 44:440-446   DOI   ScienceOn
16 Conti, M., D'Agostino, G, Caserta, A. and Mela, L. 1988. Some characteristics of chrysanthemum yellows disease. Acta Hort. 234:129-136
17 Vorackova, Z. P., Fraova, J., Valova, P., Mertelik, J., Narail, M. and Nebesaova, J. 1998. Identification of phytoplasma infecting Lilium martagon in the Czech Republic. J. Phytopathol. 146:609-612   DOI   ScienceOn
18 Deng, S. and Hiruki, C. 1991. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes. J. Microbiol. Methods 14:53-61   DOI   ScienceOn