Browse > Article
http://dx.doi.org/10.5423/PPJ.2005.21.1.027

Identification, Growth and Pathogenicity of Colletotrichum boninense Causing Leaf Anthracnose on Japanese Spindle Tree  

Lee, Hyang-Burm (Department of Biological Sciences, Seoul National University)
Park, Jae-Young (Department of Biological Sciences, Seoul National University)
Jung, Hack-Sung (Department of Biological Sciences, Seoul National University)
Publication Information
The Plant Pathology Journal / v.21, no.1, 2005 , pp. 27-32 More about this Journal
Abstract
Leaf anthracnose was observed on leaves of Japanese spindle tree in Seoul, Korea from autumn 2003 to spring 2004. The causal fungus was purely isolated from he leaf spot lesions and cultured on PDA. The colony on PDA was cream to orange but blackish in the center n old cultures. Conidia were formed in blackish orange asses and were cylindrical in shape, measured 13-17${\times}$5-7 ${\mu}$m in size. Blackish brown setae were often observed on PDA and ranged up to 100 ${\mu}$m in length. Based on morphological and ITS region sequence analyses, the fungal strain was identified as Colletotrichum boninense. Koch’s postulates were fulfilled by inoculating tree leaves with 1 ${\times}$ $106^6$ conidia per ml in a moist chamber. This is the first study on the pathogenicity, growth and phylogenetic characteristics of C. boninense causing leaf anthracnose on Japanese spindle tree in Korea.
Keywords
Colletotrichum boninense; Euonymus japonica; ITS region sequence; leaf anthracnose;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chun J. 1995. Computer-assisted classification and identification of actinomycetes. PhD thesis, Univ. of New Castle
2 Crous, P. W., Denman, S., Taylor, J. E., Swart, L. and Palm, M. E. 2004. Cultivation and diseases of Proteaceae: Leucadendron, Leucospermum and Protea. Centraalbureau voor Schimmelcultures, Utrecht. 227 p
3 Grand, L. F. 1985. North Carolina Plant Disease Index. North Carolina Agric. Res. Serv. Techn. Bull. 240:1-157
4 International Seed Testing Association (ISTA). 1976. Seed Sci. Technol. 4:3-49
5 Lee, H. B., Park, J. Y. and Jung, H. S. 2005. First report of leaf anthracnose caused by Colletotrichum boninense on spindle trees. Plant Pathol. 54, in press
6 Mahoney, M. J. and Tattar, T. A. 1980. Identification, etiology, and control of Euonymus fortunei anthracnose caused by Colletotrichum gloeosporioides. Plant Dis. 64:854-856   DOI
7 Shivas, R. G., Bathgate, J. and Podger, F. D. 1998. Colletotrichum xanthorrhoeae sp. nov. on Xanthorrhoea in Western Australia. Mycol. Res. 102:280-282   DOI   ScienceOn
8 Sutton, B. C. 1992. The genus Glomerella and its anamorph Colletotrichum. In: Colletotrichum: biology, pathology and control, ed. by J. A., Bailey and M. J. Jeger, pp. 1-26. CAB International, Wallingford, UK
9 Swofford, D. L. 2002. PAUP: Phylogenetic analysis using parsimony, Version 4.0b10. Illinois Natural History Survey, Champaign, IL
10 Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgin, D. G. 1997. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 24:4876-4882
11 von Arx, J. A. 1957. Die Arten der Gattung Colletotrichum Corda. Phytopathol. Z. 29:413-468
12 Barnett, H. L. and Hunter, B. B. 1987. Illustrated genera of imperfect fungi. 4th ed. Macmillan Publishing Company. NewYork, USA
13 Korea Forest Research Institute. 1991. Insect Pest and Disease of Trees and Shrubs. p. 280. Korea Forest Service
14 Alfieri Jr, S. A., Langdon, K. R., Wehlburg, C. and Kimbrough, J. W. 1984. Index of Plant Disease in Florida (Revised). Florida Dept. Agric. and Consumer Serv., Div. Plant Ind. Bull. 11: 389
15 Hilton, S. 2000. Canadian Plant Disease Survey. Agriculture and Agri-Food Canada 80:151
16 Sutton, B. C. 1980. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew, UK, pp. 523-527
17 von Arx, J. A. 1981. The genera of fungi sporulating in pure culture. 3rd ed. Cramer, Vaduz, Germany, pp. 220-223
18 Holliday, P. 1980. Fungus disease of tropical crops. Cambridge University Press, Cambridge, UK
19 Lu, G., Cannon, P. F., Reid, A. and Simmons, C. M. 2004. Diversity and molecular relationships of endophytic Colletotrichum isolates from the Iwokrama Forest Reserve, Guyana. Mycol. Res. 108:53-63   DOI   ScienceOn
20 White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, ed. by M. A. Innis, D. H. Gelfand and J. J. Sninsky, pp. 315-322. Academic Press, London, UK
21 van der Aa, H. 1978. A leaf spot disease of Nymphaea albu in the Netherlands. Neth. J. Plant Pathol. 84:109-115   DOI
22 Waller, J. M., Bridge, B. D., Black, R. and Hakiza, G. 1993. Characterization of the coffee berry disease pathogen, Colletotrichum kahawae sp. nov. Mycol. Res. 97:989-994   DOI   ScienceOn
23 USDA, 1960. Index of plant diseases in the United States. Agric. Handb. No. 165. US Gov. Print. Off., Washington, DC. 531 pp
24 Moriwaki, J., Sato, T. and Tsukiboshi, T. 2003. Morphological and molecular characterization of Colletotrichum boninense sp. nov. from Japan. Mycoscience 44:47-53   DOI   ScienceOn