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http://dx.doi.org/10.5423/PPJ.2004.20.4.308

The Application of RNA Transcript Conformation Polymorphism in Resolving Mixed Infection of PVY Isolates  

Maslenin, Ludmila (Virology Department, ARO, the Volcani Center)
Rosner, Arie (Virology Department, ARO, the Volcani Center)
Publication Information
The Plant Pathology Journal / v.20, no.4, 2004 , pp. 308-312 More about this Journal
Abstract
A method based on RNA-transcript conformation polymorphism (TCP) was tested for detection of two PVY isolates in a mixed infection. Differences in electrophoretic mobility of RNA transcripts copied from PCR products of each virus isolate enabled the distinction between the two virus isolates in a mixed infection. The identities of the RNA transcripts and hence of the infecting virus isolates were determined by annealing to reference oligonucleotides containing unique strain-specific sequences visualized by retardation of transcript mobility in gel. The ratio at which both virus isolates could be detected was as low as 1:10. The suitability of this procedure for the study of mixed virus infections is discussed.
Keywords
Potato virus Y; rSSCP; strain differentiation; transcript conformation polymorphism;
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