Browse > Article
http://dx.doi.org/10.5423/PPJ.2004.20.4.283

Characterization of Korean Erwinia carotovora Strains from Potato and Chinese Cabbage  

Seo, Sang-Tae (Division of Horticultural Environment, Natinal Horticultural Research Institute, RDA.)
Koo, Jun-Hak (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Hur, Jang-Hyun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Lim, Chun-Keun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.20, no.4, 2004 , pp. 283-288 More about this Journal
Abstract
Four Erwinia carotovora strains isolated from potatoes showing blackleg symptoms and rotted Chinese cabbage were analysed by biochemical tests and sequence analysis of 16S rDNA and 16S-23S rRNA intergenic spacer (IGS) regions, and the data were compared to related E. carotovora strains. Based on the results of the biochemical tests and sequence analysis, 2 of the 4 strains were identified as E. carotovora subsp. carotovora (Ecc), whereas the rest strains were distinct from Ecc. The last two strains, HCC3 and JEJU, were biochemically similar to E, carotovora subsp. atroseptica (Eca). However, the results of sequence analysis and Eca-specific PCR assays showed that the strains were distinct from Eca. On the basis of 16S rDNA sequence analysis, HCC3 and JEJU strains were placed in E. carotovora subsp. odorifera and E. carotovora subsp. wasabiae, respectively. The results of sequence analysis and specific PCR assay for Eca indicated that Asian Eca strains were distinct from European Eca strains, although they were phenotycally homogeneous.
Keywords
Chinese cabbage; Erwinia carotovora; PCR; potato;
Citations & Related Records
연도 인용수 순위
  • Reference
1 De Boer, S. H. and Ward, L. J. 1995. PCR detection of Envinia carotovora subsp. atroseptica associated with potato tissue Phytopathology 85:854-858   DOI   ScienceOn
2 Hauben, L., Moore, E. R. B., Vauterin, L., Steenackers, M., Mergaert, J., Verdonck, L. and Swings, J. 1998. Phylogenetic position of phytopathogens within the Enterobacteriaceae. System. Appl. Microbiol. 21:384-397   DOI   ScienceOn
3 Nakagawa, T., Shimada, M., Mukai, H., Asada, K., Kato, I., Fuhino, K. and Sato, T. 1994. Detection of alcohol-tolerant Hiochi bacteria by PCR. Appl. Environ. Microbiol. 60:637-640
4 Fessehaie, A., De Boer, S. H. and Levesque, C. A. 2002. Molecular characterization of DNA encoding 16S-23S rRNA intergenic spacer regions and 16S rRNA of pectolytic Envinia species. Can. J Microbiol. 48:387-398   DOI   ScienceOn
5 Toth, I. K., Avrova, A. O. and Hyman, L. J. 2001. Rapid identification and differentiation of the soft rot Erwinias by 16S-23S intergenic transcribed spacer-PCR and restriction fragment length polymorphism analyses. Appl. Environ. Microbiol. 67:4070-4076   DOI   ScienceOn
6 Duarte, V., De Boer, S. H., Ward, L. J. and De Oliveira, A. M. R. 2004. Characterization of atypical Erwinia carotovora strains causing blackleg of potato in BraZil. J Appl. Microbiol. 96:535-545   DOI   ScienceOn
7 Asai, T., Zaporojets, D., Squires, C. and Squires, C. L. 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchangeof rRNA genes between bacteril. Proc. Natl. Acad. Sci. US.A. 96:1971-1976   DOI   ScienceOn
8 De Boer, S. H., Cuppels, D. A. and Kelman, A. 1978. Pectolytic Erwinia spp. in the root zone of potato plants in relation to infestation of daughter tubers. Phytopathology 68: 1784-1790   DOI
9 Darrasse, A., Priou, S., Kotoujansky, A. and Bertheau, Y. 1994. PCR and restriction length polymorphism of a pel gene as a tool to identitY Erwinia carotovora in relation to potato diseases. Appl. Environ. Microbiol. 60: 1437-1443
10 Lelliot, R. A. and Dickey, R. S. 1984. Genus VII. Erwinia Winslow,Broadhurst, Buchanan, Krumwiede, Rogers and Smith1920, $209^A^L$In Krieg, N. R., Holt, J.G eds, Bergey's manual of systematic bacteriology, vol. 1. Baltimore, USA
11 Condon, C., Squires, C. and Squires, C. L. 1995. Control of rRNA transcription in Escherichia coli. Microbiol. Rev. 59:623-645
12 Dallagi, A., Birch, P. R. J., Heilbronn, J., Lyon, G and Toth, I. K. 2000. cDNA-AFLP analysis of differential gene expression in the bacterial plant pathogen Envinia carotovora. Microbiology 146:165-171
13 Perombelon, M. C. M. and Salmond, G P.C. 1995. Bacterial soft rots, p. 1-17. In ingh, U. S., Singh, R. P. and Kohmoto, K. (ed.), Pathogenesis and host specificity in plant diseases, vol. 1. Pergamon Press, Oxford, England
14 De Boer, S. H. and Kelman, A. 2000. Gram-negative bacteria: Erwinia soft rot group. In Laboratory Guide for Identification of Plant Pathogenic Bacteria, 3rd edn. ed. Schaad, N. W, Jones, J. B. and Chun, W pp. 56-72. St Paul, MN: American Phytopathological Society
15 Seo, S. T., Furuya, N., Lim, C. K. Lim, Y. and Tsuchiya, K. 2002. Phenotypic and genetic diversity of Erwinia carotovora ssp. carotovora strains from Asia. J Phytopathol. 150: 120-127   DOI   ScienceOn
16 Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidmann, J. G, Smith, J. A. and Struhl. K. 1987. Current protocols in molecular biology. Greene Publishing Associates and Wiley Interscience, N. Y