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http://dx.doi.org/10.5423/PPJ.2004.20.2.131

Characterization of Cucumber mosaic virus Isolated from Water Chickweed(Stellaria aquatica)  

Park, Gug-Seoun (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration)
Kim, Jae-Hyun (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administratio)
Kim, Jeong-Soo (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administratio)
Park, Jang-Kyung (Division of Biological Environment, College of Agriculture and Life Science, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.20, no.2, 2004 , pp. 131-134 More about this Journal
Abstract
A strain of Cucumber mosaic virus (CMV) was isolated from a weed, water chickweed (Stellaria aquatica), growing in the pepper field in Chunchon, Korea. This isolate, CMV-Sa, was differentiated from other CMVs based on biological properties and nucleotide sequence analysis of the coat protein (CP) gene. CMV-Sa showed different reactions to all the tested plants, except Capsicum annuum and Cucumis sativus, when compar-ed with those of CMV-Mf (subgroup I) and CMV-PaFM (subgroup II). Remarkably, in Nicotiana tabacum cvs. Samsun, Xanthi-nc and Ky-57, CMV-Sa induced local necrotic ring spots on the inoculated leaves and venal wave pattern and mosaic on the upper leaves. RNA analysis, serology, and RT-PCR of CP gene showed that CMV-Sa belonged to subgroup I of CMV. However, restriction enzyme analysis of the cDNA using AluI, HhaI, HincII, HindIII, HinfI and MspI showed that CMV-Sa was distinct from that of CMV-Mf. Based on comparison of the nucleotide of CP gene and deduced amino acid sequences between other CMV strains, CMV-Sa was closely related to CMV-Mf with 93.7% and 97.2 % identity, respectively.
Keywords
Cucumber mosaic virus (CMV); Stellaria aquatica; subgroup; water chickweed;
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1 Boccard, F. and Baulcombe, D. C. 1993. Mutational analysis of cis-acting sequences and gene function in RNA3 of cucumber mosaic virus. Virology. 193:563-578   DOI   ScienceOn
2 Ding, B., Li, Q., Nguyen, L., Palukaitis, P. and Lucas, W. J. 1995. Cucumber mosaic virus (CMV) 3a protein potentiates cell-to-cell trafficking of CMV RNA in tobacco plants. Virology. 207:345-353   DOI   ScienceOn
3 Ding, S., Li, W. and Symons, R. H. 1995. A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J. 14:5762-5772
4 Gonda, T. J. and Symons, R. H. 1978. The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: Its application to several strains of cucumoviruses. Virology. 88:361-370   DOI   ScienceOn
5 Hayes, R. J. and Buck, K. W. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363-368   DOI   ScienceOn
6 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348   DOI
7 Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. New York, Cold Spring Harbor
8 Takanami, Y 1989. A striking change in symptoms of cucumber mosaic virus-infected tobacco plants induced by a satellite RNA. Virology 109:120-126   DOI   PUBMED   ScienceOn
9 Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumovirus with a genus specific single pair of primers. J. Virol. Methods 83:67-73   DOI   ScienceOn
10 Kim, J. H., Choi, G. S. and Choi, J. K. 2002. Characterization of cucumber mosaic virus subgroup II isolated from paprika (Capsicum annum var. grossum) in Korea. Plant PathoI' J. 18:6-11   DOI   ScienceOn
11 Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification of cucumber mosaic virus isolates in Korea. Plant PathoI' J. 14:7-12
12 Ryu, K. H., Park, W. M. and Choi, J. K. 2002. Characterization and sequence analysis of lily isolate of Cucumber mosaic virus from Lilium tsingtauense. Plant PathoI. J. 18:85-92   DOI   ScienceOn
13 Nitta, N., Takanami, Y., Kuwata, S. and Kubo, S. 1988. Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J. Gen. Virol. 69:2695-2700   DOI
14 Schmitz, I. and Rao, A. L. N. 1998. Deletions in the conserved amino-terminal basic arm of cucumber mosaic virus coat protein disrupt virion assembly but do not abolish infectivity and cell-to-cell movement. Virology. 248:323-331   DOI   ScienceOn
15 Brigneti, G., Voinnet, O., Li, W. X., Ji, L. H., Ding, S. W. and Baulcombe, D. C. 1998. Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J. 16:6739-6746
16 Roossinck, M. J. 1999. Cucumoviruses, pp 315-320. In A. Granoff, and R. G. Webster (ed.), Encyclopedia of virology, 2nd ed., vol. 1. Academic Press, Inc., San Diego, Calif
17 Kaplan, I. B., Zhang, L. and Palukaitis, P. 1998. Characterization of cucumber mosaic virus. V Cell-to-cell movement requires capsid protein but not virions. Virology 246:221-231   DOI   PUBMED   ScienceOn
18 Anonymous, 1998. Detection and biodiversity of cucumber mosaic cucumovirus. J. Plant PathoI. 80: 133-149
19 Roossinck, M. J., Zhang, L. and Hellwald, K H. 1999. Rearrangement in the 5'nontranslated region and phylogenetic analysis of cucumber mosaic virus RNA3 indicated radial evolution of three subgroup. J. ViroI. 73:6752-6758
20 Jung, H. J., Ueda, S., Ryu, K. H., Lee, S. Y. and Choi, J. K. 2000. A novel strain of cucumber mosaic virus isolated from Lilium longgiflorum. Plant PathoI.2000 J. 16:306-311
21 Edwards, M. C. and Gonsalves, D. 1983. Grouping of seven biological defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology. 73: 1117-1120   DOI
22 Shintaku, M. and Palukatis, P. 1990. Genetic mapping of cucumber mosaic virus. In Viral Gene and Plant Pathogenesis, ed. by Pirone, T. P and Shaw, J. G., pp 157-164. Spriger-Verlag, New York