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http://dx.doi.org/10.5423/PPJ.2003.19.6.269

Vegetative Compatibility Grouping and Pathogenicity of Colletotrichum gloeosporioides Isolates from Different Host Plants  

Ahn, Il-Pyung (School of Agricultural Biotechnology, Seoul National University)
Kim, Soonok (School of Agricultural Biotechnology, Seoul National University)
Im, Kyung-Hwan (Department of Biology, University of Incheon)
Lee, Yong-Hwan (School of Agricultural Biotechnology, Seoul National University)
Publication Information
The Plant Pathology Journal / v.19, no.6, 2003 , pp. 269-273 More about this Journal
Abstract
A total of 57 isolates of Colletotrichum gloeosporioides were recovered from diseased tissues of Hall's crab apple (Malus haliana), 3 cultivars of edible apple (M. pumila var. dulcissima), red pepper (Capsicum annum), and grapevine (Vitis vinifera) fruits. All isolates showed strong virulence on their own host plants. Isolates from edible apple exhibited high level of cultivar specificity in pathogenicity tests. Ten isolates from apple cultivar 'Fuji' were virulent on 'Jonathan' and 'Rall's Genet'. However, 12 isolates from 'Jonathan' and 'Rall's Genet' were not virulent on 'Fuji'. Among the 24 isolates from red pepper, only seven and two isolates were infective on edible apple and grapevine fruits, respectively. All six isolates from grapevine were only virulent on their own host. These isolates were grouped into five vegetative compatibility groups (VCGs), A, B, C, D, and E, by demonstrating heterokaryosis through complementation using nitrate-nonutilizing (nit) mutants. Among them, isolates belong to VCG-A and VCG-D accounted for 24 and 17 isolates； those in VCG-A exhibited wide host range involving Hall's crab apple, all three edible apple cultivars, and red pepper. On the other hand, isolates of VCG-D and VCG-E showed limited host range specific to red pepper and grapevine, respectively. Taken together, the data suggest that among C. gloeosporioides isolates, the concepts of pathotype and/or forma specialis may exist, and that three is a relationship between host specificity and VCG grouping among C. gloeosporioides isolates.
Keywords
Glomerella cingulata; host range; mycelial compatibility group; host range;
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1 Davis, R. D., Irwin, J. A. G. and Cameron, D. F. 1984. Variation in virulence and pathogenic specialization of Colletotrichum gloeosporioides isolates from Stylosanthes scabra cv. Fitzroy and Seca. Aust. J. Agric. Res. 35:653-662   DOI
2 Kelemu, S., Skinner, D. Z., Badel, J. L., Moreno, C. X., Rodriguez, M. X., Fernandes, C. D., Charchar, M. J. and Chakraborty, S. 1999. Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume. Eur. J. Plant Pathol. 105:261-272   DOI
3 Larkin, R. P., Hopkins, D. L. and Martin, F. N. 1990. Vegetative compatibility within Fusarium oxysporum f. sp. niveum and its relationship to virulence, aggressiveness, and race. Can. J. Microbiol. 36:352-358   DOI
4 Liu, W. and Sundheim, L. 1996. Nitrate nonutilizing mutants and vegetative compatibility groups in Fusarium poae. Fungal Genet. BioI. 20:12-17   DOI   ScienceOn
5 Oh, I.-S. 1995. Taxonomy and pathogenicity of Colletotrichum spp. from red pepper (Capsicum annuum L.). 105p. Chungnam National University, Taejon, Korea. (Ph. D. Thesis)
6 Park, E. W., Hur, J. S. and Yun, S. C. 1992. A forecasting system for scheduling fungicide sprays to control grape ripe rot caused by Colletotrichum gloeosporioides. Korean J. Plant Pathol. 8:177-184
7 von Arx, J. A. 1957. Die arten der Gattung Colletotrichum cda. Phytopathol. Z. 29:413-468
8 Wasilwa, L. A., Correll, J. C., Morelock, T. E. and McNew, R. E. 1993. Reexamination of races of the cucurbit anthracnose pathogen Colletotrichum orbiculare. Phytopathology 83:1190-1198   DOI   ScienceOn
9 Daniel,.J.T., Templeton, G. E., Smith, R. J. and Fox, W. T. 1973. Biological control of Northern jointvetch in rice with an endemic fungal disease. Weed Science 21:303-307
10 Cisar, C. R., Spiegel, F. W., TeBeest, D. O. and Trout, C. 1994. Evidence for mating between isolates of Colletotrichum gloeosporioides with different host specificities. Curr. Genet. 25:330-335   DOI   ScienceOn
11 Ahn, I.-P., Chung, H.-S. and Lee, Y.-H. 1998. Vegetative compatibility groups and pathogenicity among isolates of Fusarium oxysporum f. sp. cucumerinum. Plant Dis. 82:244-246   DOI   ScienceOn
12 TeBeest, D. O. 1988. Additions to host range of Colletotrichum gloeosporioides f. sp. aeschynomene. Plant Dis. 72: 16-18   DOI
13 Farr, D., Bills, G. F., Chamuris, G. P. and Rossman, A. Y. 1989. Fungi on plants and plant products in the United States. American Phytopathological Society, St. Paul, Minnesota, USA
14 Delp, C. J. 1980. Coping with resistance to plant disease control agents. Plant Dis. 64:652-657   DOI
15 Sutton, B. C. 1980. The Coelomycetes. Commonw. Mycol. Inst. Kew, England
16 Brooker, N. L., Leslie, J. F. and Dickman, M. B. 1991. Nitrate nonutilizing mutants of Colletotrichum and their use in studies of vegetative compatibility and genetic relatedness. Phytopathology 81:672-677   DOI
17 Jacobson, D. J. and Gordon, T. R. 1991. Fusarium oxysporum f. sp. melonis: a case study of diversity within a forma specialis. Phytopathology 81: 1064-1067
18 Freeman, S., Katan, T. and Shabi, E. 1996. Characterization of Colletotrichum gloeosporioides isolates from avocado and almond fruits with molecular and pathogenicity tests. Appl. Environ. Microbiol. 62: I014-1020
19 Beynon, S. M., Coddington, A., Lewis, B. G. 'and Varzea, V. 1995. Genetic variation in the coffee berry disease pathogen, Colletotrichum kahawae. Physiol. Mol. Plant Pathol. 46:457-470   DOI   ScienceOn
20 Park, K. S. and Kim, C. H. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean J. Plant Pathol. 8:61-69
21 Vaillancourt, L. J. and Hanau, R. M. 1994. Nitrate nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola (Colletotrichum graminicola). Exp. Mycol. 18:311-319   DOI   ScienceOn
22 Alahakoon, P. W. and Brown, A. E. 1994. Host range of Colletotrichum gloeosporioides on tropical fruit crops in Sri Lanka. International J Pest Management 40:23-26   DOI   ScienceOn
23 Bames, G. L. 1972. Differential pathogenicity of Oklahoma isolates of Colletotrichum orBiculare. Plant Dis. Rep. 50:35-38
24 Freeman, S. and Shabi, E. 1996. Cross-infection of subtropical and temperate fruits by Colletotrichum from various hosts. Physiol. Mol. Plant Pathol. 49:395-404   DOI   ScienceOn
25 Park, H. K., Kim, B. S. and Lee, W. S. 1990. Inheritance of resistance to anthracnose (Colletotrichum spp.) in pepper (Capsicumannuum L.). J. Kor. Soc. Hort. Sci. 31:91-105
26 Bernstein, B., Zehr, E. I., Dean, R. A. and Shabi, E. 1995. Characteristics of Colletotrichum from peach, apple, pecan, and other hosts. Plant Dis. 79:478-482   DOI   ScienceOn
27 Chacko, R. J., Weidemann, G. J., TeBeest, D. O. and Correll, J. C. 1994. The use of vegetative compatibility and heterokaryosis to determine potential asexual gene exchange in Colletotrichum gloeosporioides. Biol. Control 4:328-389   DOI   ScienceOn
28 Oh, B.-J., Kim, K.-D. and Kim, Y.-S. 1998. A microsocopic characterization of the infection of green and red pepper fruits by an isolate of Colletotrichum gloeosporioides. J Phytopathol. 146:301-303   DOI   ScienceOn
29 Goode, M. J. 1958. Physiological specialization in Colletotrichum lagenarium. Phytopathology 48:79-83
30 Anagnostakis, S. L. 1987. Chestnut blight: the classical problem of an introduced pathogen. Mycologia 79:23-27   DOI
31 Puhalla, J. E. 1985. Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Can. J. Bot. 62:540-545   DOI