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http://dx.doi.org/10.5423/PPJ.2003.19.4.210

A Subpopulation of RNA3 of Cucumber mosaic virus Quasispecies  

Park, Seung-Kook (Department of Biochemistry and Biophysics, Texas A&M University)
Park, Sun-Hee (Plant Virus GenBank, PVGABC, Division of Life and Environmental Sciences, Seoul Women's University)
Yoon, Ju-Yeon (Plant Virus GenBank, PVGABC, Division of Life and Environmental Sciences, Seoul Women's University)
Park, Jang-Kyung (Division of Biological Environment, Kangwon National University)
Ryu, Ki-Hyun (Plant Virus GenBank, PVGABC, Division of Life and Environmental Sciences, Seoul Women's University)
Publication Information
The Plant Pathology Journal / v.19, no.4, 2003 , pp. 210-216 More about this Journal
Abstract
This study examined the existence of genetically diverse population of Cucumber mosaic virus (CMV), known as quasispecies, from lily, Nicotiana benthamiana and from purified virions. Based on the conserved sequences of CMV lily isolates in intergenic region (IR) on RNA3, the genetic variation of IR from three different sources was investigated by a specific restriction endonuclease hydrolysis of amplified reverse transcription-polymerase chain reaction (RT-PCR) products using virus-specific primers, and was compared with IR sequences. The IR nucleotide sequences of CMV lily isolates were highly conserved, however, quasispecies was detected from all three sources in low level, containing sub-populations of RNA3. These subpopulations of RNA3 were inoculated onto zucchini squash by in vitro transcripts from corresponding full-length cDNA clones together with Eny RNA1 and 2 transcripts. The systemic symptom of zucchini plants infected by these quasispecies was chlorotic spotting, which was milder than severe mosaic and stunt symptom caused by Eny-CMV. The severity of symptom was correlated with RNA accumulation of viruses. These results suggest that the genome of CMV lily isolates consists of quasispecies populations.
Keywords
Cucumber mosaic virus; intergenic region; lily; quasispecies; subpopulation;
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1 Canto, T., Proir, T.A., Hellwald, K.H., Oparka, K. and Palukaitis, P. 1997. Characterization of cucumber mosaic virus. IV. Movement protein and coat protein are both essential for cell-to-cell movement of cucumber mosaic virus. Virology 237:237-248   DOI   PUBMED   ScienceOn
2 Chen, Y.K. Derks, A.F.L.M., Langeveld, S., Goldbach, R. and Prins, M. 2001. High sequence conservation among cucumber mosaic virus isolates from lily. Arch. Virol. 146:1631-1636   DOI   ScienceOn
3 Chen, Y.K., Goldbach, R. and Prins, M. 2002. Inter- and intramolecular recombinations in the cucumber mosaic virus genome related to adaptation to alstroemeria. J. Virol. 76:4119-4124   DOI   ScienceOn
4 Gal-On, A., Kaplan, I., Roossinck, M. and Palukaitis, P. 1995. Differential effects of satellite RNA on the accumulation of cucumber mosaic virus RNAs and their encoded proteins in tobacco vs. zucchini squash with two strains of CMV helper virus. Virology 208:58-66   DOI   ScienceOn
5 Hayes, R.J. and Buck, K.W. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363-368   DOI   ScienceOn
6 Jung, H.J., Veda, S., Ryu, K.H., Lee, S.Y. and Choi, J.K. 2000. A novel strain of cucumber mosaic virus isolated from Lilium longiflorum. Plant Pathol. J. 16:306-311
7 Liu, S., He, X., Park, G., Josefsson, C. and Perry, K.L. 2002. A conserved capsid protein surface domain of cucumber mosaic virus is essential for efficient aphid vector transmission. J. Virol. 76:9756-9762   DOI   ScienceOn
8 Palukaitis, P. and Roossinck, M.J. 1995. Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virus and the initial sequence context. Virology 206:765-768   DOI   ScienceOn
9 Palukaitis, P., Roossinck, M.J., Dietzgen, R.G. and Francki, R.I.B.1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348   DOI
10 Roossinck, M.J. and Palukaitis, P. 1991. Differential replication in zucchini squash of a cucumber mosaic virus satellite RNA maps to RNA 1 of the helper virus. Virology 181:371-373   DOI   ScienceOn
11 Roossinck, M.J. 1997. Mechanism of plant virus evolution. Annu. Rev. Phytopathol. 35: 191-209   DOI   PUBMED   ScienceOn
12 Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York
13 Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467   DOI   ScienceOn
14 Schneider, W.L. and Roossinck, M.J. 2001. Genetic diversity in RNA virus quasispecies is controlled by host-virus interactions. J. Virol. 75:6566-6571   DOI   ScienceOn
15 Gal-On, A., Kaplan, I., Roossinck, M. and Palukaitis, P. 1994. The kinetics of infection of zucchini squash by cucumber mosaic virus indicates a function RNA1 in virus movement. Virology 205:280-289   DOI   ScienceOn
16 Rodriguez-Alvarado, G., Kurath, G. and Dodds, A. 1995. Heterogeneity in pepper isolates of cucumber mosaic virus. Plant Dis. 79:450-45524   DOI   ScienceOn
17 Kaplan, I.E., Gal-On, A and Palukaitis, P. 1997. Characterization of cucumber mosaic virus: III. Localization of sequence in the movement protein controlling systemic infection in cucurbits. Virology 230:343-349   DOI   PUBMED   ScienceOn
18 Aranda, M.A., Fraile, A and Garcia-Arenal, F. 1993. Genetic variability and evolution of the satellite RNA of cucumber mosaic virus during natural epidemics. J. Virol. 67:5896-5901
19 Schneider, W.L., Roossinck, M.J. 2000. Evolutionarily related Sindbis-like plant viruses maintain different levels of population diversity in a common host. J. Virol. 74:3130-3134   DOI   ScienceOn
20 Domingo, E., Ruiz-Jarabo, C.M., Sierra, S., Arias, A, Pariente, N., Baranowski, E. and Escarrnis, C. 2002. Emergence and selection of RNA virus variants: memory and extinction. Virus Res. 82:39-44
21 Domingo, E., Escarrnis, C., Sevilla, N., Moya, A., Elena, S.F., Quer, J., Novella, I. and Holland, J.J. 1996. Basic concepts in RNA virus evolution. FASEB J. 10:859-864
22 Masuta, C., Seshimo, Y., Mukohara, M., Jung, H.J., Veda, S., Ryu, K.H. and Choi, J.K. 2002. Evolutionary characterization of two lily isolates of Cucumber mosaic virus isolated in Japan and Korea. J. Gen. Plant Pathol. 68:163-168   DOI   ScienceOn
23 Boccard, F. and Baulcombe, D. 1993. Mutational analysis of cisacting sequences and gene function in RNA3 of cucumber mosaic virus. Virology 93:563-78
24 Carrere, I., Tepfer, M. and Jacquemond, M. 1999. Recombinants of cucumber mosaic virus (CMV): determinants of host range and symptomatology. Arch. Virol. 144:365-379   DOI   ScienceOn
25 Peden, K.W.C. and Symons, R.H. 1973. Cucumber mosaic virus contains a functionally divided genome. Virology 53:487-492   DOI   ScienceOn
26 Canto, T., Choi, S.K and Palukaitis, P. 2001. A subpopulation of cucumber mosaic virus RNA1 contains 3' termini originating from RNAs 2 or 3. J. Gen. Virol. 82:941-945   DOI
27 Rizzo, T.M. and Palukaitis, P. 1990. Construction of full-length cDNA clones of cucumber mosaic virus RNAs 1. 2. and 3:generation of infectious RNA transcripts. Mol. Gen. Genet. 222:249-256   DOI   PUBMED
28 Roossinck, M.J. and Palukaitis, P. 1990. Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA1 of cucumber mosaic virus. Mol. Plant-Microbe Interat. 3:188-192   DOI
29 Ryu, K.H., Park, H.W. and Choi, J.K. 2002. Characterization and sequence analysis of a lily isolate of cucumber mosaic virus from Lilium tsingtauense. Plant Pathol. J. 18:85-92   DOI   ScienceOn
30 Kurath, G. and Palukaitis, P. 1989. RNA sequence heterogeneity in natural populations of three satellite RNAs of cucumber mosaic virus. Virology 173:231-240   DOI   ScienceOn