Browse > Article
http://dx.doi.org/10.5423/PPJ.2003.19.3.171

Complete Genome Sequences of the Genomic RNA of Soybean mosaic virus Strains G7B and G5  

Kim, Kook-Hyung (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Lim, Won-Seok (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Kim, Yul-Ho (National Crop Experimental Station, Rural Development Adminstration)
Publication Information
The Plant Pathology Journal / v.19, no.3, 2003 , pp. 171-176 More about this Journal
Abstract
The complete nucleotide sequences of the genomic RNAs of Soybean mosaic virus strains GS (SMV-G5) and G7H (SMV-G7H) were determined and compared with sequences of other SMV strains. Each viral RNA was determined to be 9588 nucleotides in length excluding the poly (A) tail and contained an open reading frame to encode a polyprotein subsequently processed into up to ten proteins by proteolytic cleavage. Com-parison of the amino acid sequences with those of other SMV strains showed high percentage of amino acid sequence homology with the same genome organization. The nucleotide and the deduced amino acid sequences between SMV-G5 and SMV-G7H were greater than 99% identity. When compared with those of other SMV strains in a phylogenetic analysis of the nucleotide and deduced amino acid sequences, they formed a distinct virus clade showing over 97％ amino acid identity, but were more distantly related to the other potyvirus (44.1-69.6% identity). Interestingly, SMV G7H strain caused a severe mosaic or necrosis symptom in soybean cultivars including Jinpum-1, Jinpum-2, and Sodam, whereas, no symptom was observed in SMV-G5 inoculation. Complete nucleotide sequences of these strains will give clues for determining symptom determinant(s) in future research.
Keywords
SMV; strains G5 and G7H; genome sequence; phylogeny;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Gunduz, I., Buss, G. R., Chen, P. and Tolin, S. A. 2002. Characterization of SMV resistance genes in Tousan 140 and Hourei soybean. Crop Sci. 42:90-95   DOI   ScienceOn
2 Lee, K. C. and Wong, S. M. 1998. Variability of P1 protein of zucchini yellow mosaic virus for strain differentiation and phylogenetic analysis with other potyviruses. DNA sequence 9:275-293   DOI   ScienceOn
3 Lim, S. M. 1985. Resistance to Soybean mosaic virus in soybean. Phytopathology 75: 199-201   DOI
4 Liu, J., Peng, X., Li, L. and Mang, K. 1993. Cloning of coat protein gene of soybean mosaic virus and its expression in Escherichia coli. Chin. J. Biotechnol. 9:143-149
5 Thompson, J. D., Higgins, D. J. and Gibson, T. J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680   DOI   ScienceOn
6 Cho, E. K., Choi, S. H. and Cho, W. T. 1983. Newly recognized soybean mosaic virus mutants and sources of resistance in soybean. Res. Rept. ORD (S.P.M.U.) 25: 18-22
7 Chung, B. J. Lee, S. H. and Sung, J. M. 1973. An investigation on soybean mosaic virus: Varietal resistance and transmission. Symp. Pl. Environ. Res. Summ. pp. 73-77
8 Jung, H. W., Yun, W. S., Hahm, Y. I. and Kim, K. H. 2002. Characterization of Tobacco mosaic virus isolated from potato showing yellow leaf mosaic and stunting symptom in Korea. Plant Dis. 86: 112-117   DOI   ScienceOn
9 Yun, W. S., Jung, H. W., Oh, M. H., Hahm, Y. I. and Kim, K. H. 2002. Variation of Potato virus Y isolated from potato, tobacco, pea and weeds in Korea on the C-terminal region of coat protein gene and 3' non-translated region. Plant Pathol. J. 18:130-137   DOI   ScienceOn
10 Cho, E. K. and Goodman, R. M. 1982. Evaluation of resistance on soybeans to Soybean mosaic virus strains. Crop Sci. 22: 1133-1136   DOI
11 Kendrick, J. B. and Gardner, M. W. 1924. Soybean mosaic; Seed transmission and effect on yield. J. Agric. 27:91-98
12 Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3444   DOI   ScienceOn
13 Chung, B. J., Lee, S. H., Cho, E. K. and Park, H. C. 1974. Identification of soybean viruses and soybean varietal reactions. Ann. Rept. Crop lmpro. Res. Center pp. 137-145
14 Gunduz, I., Buss, G. R., Ma, G., Chen, P. and Tolin, S. A. 2001. Genetic analysis of resistance to Soybean mosaic virus in OX670 and Harosoy soybean, Crop Sci. 41: 1785-1791   DOI   ScienceOn
15 Page, R. D. 1996. Tree View: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12:357-358
16 Ross, J. P. 1967. Purification of Soybean mosaic virus for antiserum production. Phytopathology 57:465-467
17 Cho, E. K. and Goodman, R. M. 1979. Strains of soybean mosaic virus: Classification based on virulence in resistant soybean cultivars. Phytopathology 69:467-470   DOI
18 Riechmann, J. L., Lasin, S. and Garcia, J. A. 1992. Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 73: 116
19 Cho, E. K. and Chung, B. J. 1976. Studies on identification and classification of soybean virus diseases in Korea. Kor. J. Plant Prot. 15:61-68
20 Tordo, V. M. J., Chachulska, A. M., Fakhfakh, H., LeRomanced, M., Robaglia, C. and Asteir-Manifacier, S. 1995. Sequence polymorphism in the 5'UTR and in the PI coding region of potato virus Y genomic RNA. J. Gen. Virol. 76:939-949   DOI   ScienceOn
21 Jayaram, c., Hill, J. H. and Miller, W. A. 1992. Complete nucleotide sequences of two soybean mosaic virus strains differentiated by response of soybean containing the Rsv resistance gene. J. Gen. Virol. 73:2067-2077   DOI   ScienceOn
22 Shukla, D. D., Ward, C. W. and Brunt A. A. 1994. The Potyviridae. CAB international. Wallingford, Oxon, UK.
23 Anandalakshmi, R., Pruss, G. J., Ge, X., Marathe, R., Mallory, A. C., Smith, T. H. and Vance, V. B. 1998. A viral suppressor of gene silencing in plant. Proc. Natl. Acad. Sci. USA 95: 13079-13084   DOI   ScienceOn
24 Carrington, J. C. and Dougherty, W. G. 1988. A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc. Natl. Acad. Sci. USA 85:3391-3395   DOI   ScienceOn
25 Atreya, C. D., Atreya, P. L. and Thornbury, D. W. 1992. Sitedirected mutations in the potyvirus HC-PRO gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology 191: 106-111   DOI   ScienceOn
26 Lin, S. S., Hou, R. F. and Yeh, S. D. 2001. Complete genome sequence and genetic organization of a Taiwan isolate of Zucchini Yellow mosaic virus. Bot. Bull. Acad. Sin. 42:243-250
27 Kim, Y. H. 2000. G7H, a new strain of Soybean mosaic virus: Its virulence and nucleotide sequence of cylindrical inclusion gene. Ph. D. thesis. Kyungpook National University. 120 p