Browse > Article
http://dx.doi.org/10.5423/PPJ.2002.18.1.018

Antiviral, Antimicrobial, and Cytotoxic Properties of Peptavirins A and B Produced by Apiocrea sp.14T  

Kim, Young-Ho (School of Agricultural Biotechnology and research Center for New Bio-Materials in Agriculture, Seoul National University)
Yeo, Woon-Hyung (Korea ginseng and Tobacco Research Institute)
Yun, Bong-Sik (Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Sook (Korea Research Institute of Bioscience and Biotechnology)
Lee, Sang-Jun (Korea Research Institute of Bioscience and Biotechnology)
Yoo, Ik-Dong (Korea Research Institute of Bioscience and Biotechnology)
Kim, Kab-Sig (Korea ginseng and Tobacco Research Institute)
Park, Eun-Kyung (Korea ginseng and Tobacco Research Institute)
Lee, Jong-Chull (Korea ginseng and Tobacco Research Institute)
Publication Information
The Plant Pathology Journal / v.18, no.1, 2002 , pp. 18-22 More about this Journal
Abstract
Two peptaibol antibiotics, peptavirins A and B, which exhibited strong inhibitory effect against Tobacco mosaic vials (TMV) infection, were isolated from steam-cooked rice culture of Apiocrea sp.14T. The peptavirins were identified as new derivatives of chrysospermins, which are 19-mer and have been reported to be produced in a fungal isolate. The physicochemical properties of the peptavirins were mostly identical with chrysospermins A through D except for the UV absorption spectrum. The peptavirins inhibited the growths of the Grampositive bacteria tested, including the plant pathogenic bacterium, Corynebacterium lilium, and the fungus, Aspergillus niger. Peptavirin A was somewhat cytotoxic to cancer cell lines, especially K562 (leukemia) and UACC 62 (melanoma), whereas peptavirin B only exhibited slight cytotoxicity.
Keywords
antimicrobial activity; Apiocrea sp.; cytotoxicity; peptavirins; peptaibol antibiotics.;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dornberger, K., Ihn, W., Ritzau, M., $Gr\ddot{a}fe,$ U., Schlegel, B., Fleck, W. F. and Metzger, J. W. 1995. Chrysospermins, new peptaibol antibiotics from Apiocrea chrysosperma AP 101. J. Antibiot.48:977-989   DOI
2 Kim, Y. H., Yeo, W.-H., Kim, Y.-S., Chae, S.-Y. and Kim, K.-S. 2000. Antiviral activity of antibiotic peptaibols, chrysospermins B and D, produced by Apiocrea sp. 14T against TMV infection. J. Microbiol. Biotechnol. 10:522-528
3 Snook, C. F., Woolley, G. A., Oliva, G., Pattabhi, V., Wood, S. F., Blundell, T. L. and Wallace, B. A. 1998. The structure and function of antiamoebin I, a proline-rich membrane-active polypeptide. Structure 6:783-792   DOI   ScienceOn
4 Boheim, G., Hanke, W. and Jung, G. 1983. Alamethicin pore formation: voltage dependent flip-flop of $\alpha$-helix dipoles. Biophys. Struct. Mech. 9:181-191   DOI
5 Beven, L., Duval, D., Rebuffat, S., Riddell, F. G., Bodo, B. and Wroblewski, H. 1998. Membrane penneabilisation and antimycoplasmic activity of the 18-residue peptaibols, trichorzins PA. Biochim. Biophys. Acta. 1372:78-90   DOI   ScienceOn
6 Gr$\ddot{a}$fe, U., Ihn, W., Ritzau, M., Schade, W., Stengel, C., Schlegel, B., Fleck, W. F., K$\ddot{u}$nkel, W., H$\ddot{a}$tel, A. and Gutsche, W. 1995. Helioferins, novel antifungal lipopeptides from Mycogone rosea. Screening, isolation, structures and biological properties. J. Antibiot. 48:126-133   DOI   ScienceOn
7 Balaram, P., Krishana, K., Sukumar, M., Mellor, I. R. and Sansom, M. S. 1992. The properties of ion channels formed by zervamicins. Eur. Biophys. J. 21:117-128
8 $Br\ddot{u}kner,$kner, H., Graf, H. and Bokel, M. 1984. Paracelsin; Characterization by NMR spectroscopy and circular dichroism, and hemolytic properties of a peptaibol antibiotic from the celIulolytically active mold Trichoderma reesei. Part B. Experientia 40:1189-1197   DOI   ScienceOn
9 Ritzau, M., Heinze, S., Dornberger, K., Berg, A., Fleck, W., Schlegel, B., H$\"a$rtl, and Gr$\"a$fe, U. 1997. Ampullosporin, a new peptaibol-type antibiotic from Sepedonium ampullosporum HKI-0053 with neuroleptic activity in mice. J. Antibiot. 50: 722-728   DOI   ScienceOn
10 Duval, D., Cosette, P., Rebuffat, S., Duclohier, H., Bodo, B. and Molle, G. 1998. Alamethicin-like behaviour of new 18-residue peptaibols, trichorzins PA. Role of the C-terminal amino alcohol in the ion channel forming activity. Biochim. Biophys. Acta. 1369:309-319   DOI   ScienceOn
11 Lee, S.-J., Yun, B.-S., Cho, D.-H. and Yoo, I.-D. 1999. Tylopeptins A and B, new antibiotic peptides from Tyneofelleus. J. Antibiot. 52:998-1006   DOI   ScienceOn
12 Sansom, M. S. 1993. Alamethicin and related peptaibols - model ion channels. Eur. Biophys. J. 22:105-124
13 Duval, D., Riddell, F. G., Rebuffat, S., Platzer, N. and Bodo, B. 1998. lonophoric activity of the antibiotic peptaibol trichorzin PA VI: a 23Na- and 35C1-NMR study. Biochim. Biophys. Acta 1372:370-378   DOI   ScienceOn
14 Berg, A., Ritzau, M., Iin, W., Schlegel, B., Fleck, W. F., Heinze, S. and $Gr\ddot{a}fe$, U. 1996. Isolation and structure of bergofungin, a new antifungal peptaibol from EmericelIopsis donezkii HKI 0059. J. Antibiot. 49:817-820   DOI   ScienceOn
15 Chikanishi, T., Hasumi, K., Harada, T., Kawasaki, N. and Endo, A. 1997. Clonostachin, a novel peptaibol that inhibits platelet aggregation. J. Antibiot. 50:105-110   DOI   ScienceOn
16 Menestrina, G., Voges, K. P., Jung, G. and Boheim, G. 1986. VoItage-dependent channel formation by rods of helical polypeptides. J. Membrane Biol. 93:111-132   DOI
17 Termovsky, V. I., Grigohev, P. A., Berestovsky, G. N., Schlegel, R., Dornberger, K. and Grafe, U. 1997. Effective diameters of ion channels formed by homologs of the antibiotic chrysosper min.Membr.Cell Biol. 11:497-505
18 Grigoriev, P. A., Schlegel, R., Dornberger, K. and Gr$\"a$fe, U. 1995. Formation of membrane channels by chrysospermins, new peptaibol antibiotics. Biochim. Biophys. Acta 1237:1-5   DOI   ScienceOn
19 Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J. T., Bokesch, H., Kenney, S. and Boyd, M. R. 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer Inst. 82:1107-1112   DOI
20 Yun, B.-S.,Yoo, I.-D., Kim, Y. H., Kim, Y.-S., Lee, S.-J., Kim, K. -S. and Yeo, W.-H. 2000. Peptavinns A and B, two new antiviral peptaibols against TMV infection. Tetrahedron Letters 41: 1429-1431   DOI   ScienceOn
21 Wada, S., lida, A., Asami, K., Tachikawa, E. and Fujita, T. 1997. Role of the Gln/Glu residues of trichocellins A-II/B-II in ionchannel formation in lipid membranes and catecholamine secretion from chromaffin cells. Biochim. Biophys. Acta 1325: 209-214   DOI   ScienceOn
22 Yeo, W.-H., Kim, S.-K., Kim, S.-S., Yu, S.-H. and Park, E. K. 1994. Taxonomy and fermentation of Kitasatosporia kimorexae producing new thiopeptide antibiotics, kimorexms. J. Microbiol. Biotechnol. 4:354-359
23 Cosette, P., Rebuffat, S., Bodo, B. and Molle, G. 1999. The ionchannel activity of longibrachins LGA I and LGB II: effects of Pro-2/Ala and Gln-18/Glu substitutions on the alamethicin voltage-gated membrane channels. Biochim. Biophys. Acta 1461:113-122   DOI   ScienceOn
24 Bodo, B., Rebuffat, S., el Hajji, M. and Davoust, D. 1985. Structure of trichozianine A IIIc, an antifungal peptide from Trichoderma hamanum. J. Am. Chem. Soc. 107:6011-6017   DOI
25 Leclerc, G., Rebuffat, S., Goulard, C. and Bodo, B. 1998. Directed biosynthesis of peptaibol antibiotics in two Trichoderma strains. I. Fermentation and isolation. J. Antibiot. 51: 170-177   DOI   ScienceOn