Browse > Article
http://dx.doi.org/10.5423/PPJ.2002.18.1.001

Identification of Luteovirus Nucleotide Sequences in Mild Yellow-Edge Diseased Strawberry Plants  

Shaban Montasser, Magdy (University of Kuwait, Department of Biological Sciences)
Al-Awadhi, Husain (University of Kuwait, Department of Biological Sciences)
Hadidi, Ahmed (University of Kuwait, Department of Biological Sciences)
Publication Information
The Plant Pathology Journal / v.18, no.1, 2002 , pp. 1-5 More about this Journal
Abstract
The availability of nucleotide sequences of the coat protein gene of Potato leafroll virus (PLRV) permitted the construction of DNA primers that were utilized for cDNA synthesis. Polymerase chain reaction (PCR) products of a 487 bp. and approximately 500 bp DNA fragments were amplified from nucleic acid extracts of PLRV-infected tissue and strawberry mild yellow-edge (SMYE) diseased strawberry tissue, respectively. The amplified DNA fragments were further differentiated by hybridization analysis with a CDNA probe for the coat protein gene of PLRV and restriction fragment length polymorphism (RFLP) analysis. These results suggest that a luteovirus is associated with the SMYE disease.
Keywords
Amplification; Luteovirus; PLRV; RFLP; SMYEY; strawberry.;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hadidi, A., Hansen, A. J., Parish, C. L. and Yang, X. 1991. Scar skin and dapple apple viroids are seed-borne and persistent in infected apple trees. Res. Virol. 142:289-296   DOI   ScienceOn
2 Hadidi, A., Montasser, S. and Levy, L. 1993. Detection of potato leafroll and strawberry mild yellow-edge luteoviruses by reverse transcription-polymerase chain reaction amplification. PIant Dis. 77:595-601   DOI   ScienceOn
3 Levy, L., Hadidi, A. and Garnsey, S. M. 1991. Reverse transcription polymerase chain reaction assays for the rapid detection of citrus viroids using multiplex primers sets. PhytopathoIogy 81:1212
4 Montasser, S. M., Hadidi, A., Levy, L. and Goth, R. W. 1992. A polymerase chain reaction assay for the detection of potato leafroll vims. Phytopathology 82:279-285   DOI
5 Moon, J. S., McCoppin, N. K. and Domier, L. L. 2001. Role of intergenic and 3-proximal noncoding regions in coat protein expression and replication of Barley yellow dwarf virus PAV. Plant Pathol. J. 17:22-28
6 Robertson, N. L., French, R. and Gray, S. M. 1991. Use of group specific primers and polymerase chain reaction for the detection and identification of luteoviruses. J. Gen. ViroI. 72:1473-1477   DOI   ScienceOn
7 Wetzel, T., Candress, T., Ravelonandro, M and Dunez, J. 1991. A polymerase chain reaction assay to plum pox potyvirus detection. J. Virol. Methods. 33:355-365   DOI   ScienceOn
8 Kanematsu, S., Hibi, T., Hashimoto, J. and Tsuchizaki, T. 1991. Comparison of nonradioactive cDNA probes for detection of potato spindle tuber viroid by dot-blot hybridization assay. J. Virol. Methods 35:189-197   DOI   ScienceOn
9 Langeveld, S. A., Dore, J. M., Memelink, J., Derks, A. F. L. M., van der Vlugt, C. I. M., Asjes, C. J. and Bol, J. F. 1991. Identification of potyviruses using the polymerase chain reaction with degenerate primers. J. Gen. Virol. 72:1531-1541   DOI   ScienceOn
10 Francki, R. I. B., Milne, R. G. and Hatta, T. 1985. Potato viruses. Atlas of Plant Viruses. II:159-172
11 Levy, L. and Hadidi, A. 1991. Development of reverse transcription/polymerase chain reaction assay for the detection of plum pox potyvirus from microgram quantities of total nucleic acids. Phytopathology 81:1154
12 Mellor, F. C. and Frazier, N. W. 1970. Strawberry mild yellowedge. In: Frazier, N.W. (ed.). Virus Diseases of Small Fruits and Grapevines. University of California, Berkley CA, USA. pp. 14-16
13 Yang, X., Hadidi, A. and Garnsey, S. M. 1992. Enzymatic cDNA amplification of citrus exocortis and cachexia viroids from infected citrus hosts. Phytopathology 82:279-285   DOI
14 Jelkmann, W., Martin, R. R., Lesemann, D. E., Vetten, H. J. and Skelton, F. 1990. A new potexvirus associated with strawberry mild yellow edge disease. J. Gen. Vtrol. 71:1251-1258   DOI   ScienceOn
15 Keese, P., Martin, R. R., Kawchuk, L. M., Waterhouse, P. M. and Gerlach, W. L. 1990. Nucleotide sequences of an Australian and a Canadian isolate of potato leafroll luteovirus and their relationships with two European isolates. J. Gen. Virol. 71: 719-724   DOI   ScienceOn
16 Khan, R. P. 1989. Quarantine significance: Biological considerations. In: Plant Protection and Quarantine, Vol. I. CRC Press, Boca Raton, F1, USA. pp. 201-216
17 Rigby, P. W. J., Dieckman, M., Rhodes, C. and Berg, P. 1977. Labeling deoxyribonucleic acids to high specific activity in vitro by nick translation with DNA polymerase 1. J. Mol. Biol. 113:237-251   DOI   PUBMED
18 Spiegel, S., Cohen, J. and Converse, R. H. 1986. Detection of strawberry mild yellow-edge virus by serological specific electron microscopy. Acta Hort. 186:65
19 Korschineck, I., Himmler, G., Sagl, R., Steinkellner, H. and Katinger, H. W. D. 1990. A PCR membrane spot assay for the detection of plum pox virus RNA in bark of infected trees. J. Virol. Methods 31:139-146   DOI   ScienceOn
20 Hadidi, A. and Yang, X. 1990. Detection of pome fruit viroids by enzymatic cDNA amplification. J. Virol. Methods 30:261-270   DOI   ScienceOn
21 Hadidi, A., Huang, C., Hammond, R. W. and Hashimoto, J. 1990. Homology of the agent associated with the dapple apple disease to apple scar skin viroid and molecular detection of these viroids. Phytopathology 80:163-268   DOI
22 Miller, W. A., Waterhouse, P. M. and Gerlach, W. L. 1988. Sequence and organization of barley yellow dwarf virus genomic RNA. Nucl. Acids Res. 16:6096-6111
23 van der Wilk, F., Huisman, M. J., Comelissen, B. J. C., Huttinga, H. and Goldbach, R. 1989. Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Lett. 245: 51-56   DOI   PUBMED   ScienceOn
24 Rybicki, E. P. and Hughes, F. L. 1990. Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence. J. Gen. Virol. 71:2519-2526   DOI   ScienceOn
25 Vincent, J. R., Ueng, P. p., Lister, B. A. 1990. Nucleotide sequences of coat protein genes for three isolates of barley yellow dwarf virus and their relationships to other luteovirus coat protein sequences. J. Gen. Virol. 71:2791-2799   DOI   ScienceOn
26 Mayo, M. A., Robinson, D. J., Jolly, C. A. and Hyman, L. 1989. Nucleotide sequence of potato leafroll luteovirus RNA. J. Gen. Virol. 70:1037-1051   DOI   ScienceOn