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Chemopreventive Effect of Protein Extract of Asterina pectinifera in HT-29 Human Colon Adenocarcinoma Cells  

Shon Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Nam Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Publication Information
Archives of Pharmacal Research / v.29, no.3, 2006 , pp. 209-212 More about this Journal
Abstract
We investigated the effect of protein extract of Asterina pectinifera on the activity of 4 enzymes that may playa role in adenocarcinoma of the colon: quinone reductase (QR), glutathione Stransferase (GST), ornithine decarboxylase (ODC), and cyclooxygenase (COX)-2. QR and GST activity increased in HT-29 human colon adenocarcinoma cells increased that had been exposed to 4 concentrations of the protein extract (80, 160, 200, and $240{\mu}g/mL$). Additionally, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity decreased significantly in cells exposed to the extract in concentrations of $160{\mu}g/mL$ (p<0.05), $200{\mu}g/mL$ (p<0.005), and $240{\mu}g/mL$ (p<0.005). TPA-induced COX-2 activity also decreased in cells exposed to extract concentrations of 10, 20, 40, and $60{\mu}g/mL$. COX-2 expression was also inhibited in cells exposed to this extract. These results suggest that this protein extract of A pectinifera has chemopreventive activity in HT-29 human colon adenocarcinoma cells, and therefore, may have the potential to function as a chemopreventive agent in human colorectal cancer.
Keywords
Asterina pectinifera; Cyclooxygenase-2; Glutathione S-transferase; Ornithine decarboxylase; Quinone reductase;
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1 Habig, W. H., Pabst, M. J., and Jakoby, W. B., Glutathione Stransferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249, 7130-7139 (1974)
2 Joseph, P., Xie, T., Xu, Y., and Jaiswal, A. K., NAD(P)H:quinone oxidoreductase 1 (DT-diaphorase): expression, regulation, and role in cancer. Oncol. Res., 6, 525-532 (1994)
3 Parkin, D. M., Pisani, P., and Ferlay, J., Estimates of the worldwide incidence of eighteen major cancers in 1985. Int. J. Cancer., 54, 594-606 (1993)   DOI   ScienceOn
4 Schweitzer, J., Handley, F. G., Edwards, J., Harris, W. F., Grever, M. R., Schepartz, S. A., Cragg, G., Snader, K., and Bhat, A., Summary of the workshop on drug development, biological diversity, and economic growth. J. Natl. Cancer Inst., 83, 1294-1298 (1991)   DOI
5 Shon, Y. H. and Nam, K. S., Inhibition of polyamine biosynthesis of Acanthamoeba castellanii and 12-O-tetradecanoylphorbol- 13-acetate-induced ornithine decarboxylase activity by chitosanoligosaccharide. Biotechnol. Lett., 25, 701-704 (2003)   DOI   ScienceOn
6 Shon, Y. H., Nam, K. S., and Kim, M. K., Cancer chemopreventive potential of Scenedesmus spp. cultured in medium containing bioreacted swine urine. J. Microbiol. Biotechnol., 14, 158-161 (2004)
7 Talalay, P., Fahey, J. W., Holtzclaw, W. D., Prestera, T., and Zhang, Y., Chemoprotection against cancer by phase II enzyme induction. Toxicol. Lett. (Amst.), 82, 173-179 (1995)
8 Kishimura, H. and Hayashi, K., Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera. Comp. Biochem. Physiol. B., 132, 485-490 (2002)   DOI   ScienceOn
9 Rinehart, K. L., Antitumor compounds from tunicates. Med. Res. Rev., 20, 1-27 (2000)   DOI   ScienceOn
10 Ruggieri, G. D., Drugs from the sea. Science, 194, 491-497 (1976)   DOI
11 Kawamori, T., Rao, C. V., Seibert, K., and Reddy, B. S., Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carcinogenesis. Cancer Res., 58, 409-412 (1998)
12 Marton, L. J. and Pegg, A. E., Polyamines as targets for therapeutic intervention. Annu. Rev. Pharmacol. Toxicol., 35, 55-91 (1995)   DOI   ScienceOn
13 Rao, C. V., Simi, B., and Reddy, B. S., Inhibition by dietary curcumin of azoxymethane-induced ornithine decarboxylase, tyrosine protein kinase, arachidonic acid metabolism and aberrant crypt foci formation in the rat colon. Carcinogenesis, 14, 2219-2225 (1993)   DOI   ScienceOn
14 Tanako, T., Kojima, T., Suzuki, M., and Mori, H., Chemoprevention of colon carcinogenesis by the natural product of a simple phenolic compound protocatechuic acid: suppressing effects on tumor development and biomarkers expression of colon. Cancer Res., 53, 3908-3913 (1993)
15 Liu, X. H., Wiley, H. S., and Meikle, A. W., Androgens regulate proliferation of human prostate cancer cells in culture by increasing transforming growth $factor-{\alpha}\;(TGF-{\alpha})$ and epidermal growth factor (EGF)/TGF-2 receptor. J. Clin. Endocrinol. Metab., 77, 1472-1478 (1993)   DOI
16 Marnett, L. J. and DuBois, R. N., COX-2: a target for colon cancer prevention. Annu. Rev. Pharmacol. Toxicol., 42, 55- 80 (2002)   DOI   ScienceOn