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Induction of Apoptotic Cell Death by a Ceramide Analog in PC-3 Prostate Cancer Cells  

Oh, Ji-Eun (College of Pharmacy, Chung-Ang University)
So, Kwang-Sup (College of Pharmacy, Chung-Ang University)
Lim, Se-Jin (College of Pharmacy, Dong-Duk Women's University)
Kim, Mie-Young (College of Pharmacy, Chung-Ang University)
Publication Information
Archives of Pharmacal Research / v.29, no.12, 2006 , pp. 1140-1146 More about this Journal
Abstract
Ceramide analogs are potential chemotherapeutic agents. We report that a ceramide analog induces apoptosis in human prostate cancer cells. The ceramide analog induced cell death through an apoptotic mechanism, which was demonstrated by DNA fragmentation, the cleavage of poly ADP ribose polymerase (PARP), and a loss of membrane asymmetry. Treating the cells with ceramide analog resulted in the release of various proapoptotic mitochondrial proteins including cytochrome c and Smac/DIBLO into the cytosol, and a decrease in the mitochondrial membrane potential. In addition, the ceramide analog decreased the phospho-Akt and phospho-Bad levels. The expression of the antiapoptotic Bcl-2 decreased slightly with increasing Bax to Bcl-2 ratio. These results suggest that the ceramide analog induces apoptosis by regulating multiple signaling pathways that involve the mitochondrial pathway.
Keywords
Ceramide analog; Apoptosis; Akt; Cytochrome c; Smac; BcI-2;
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Times Cited By Web Of Science : 11  (Related Records In Web of Science)
Times Cited By SCOPUS : 10
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