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Chiral Separation of ${\beta}-Blockers$ after Derivatization with a New Chiral Derivatization Agent, GATC  

Ko, Mi-Young (College of Pharmacy, Kangwon National University)
Shin, Dae-Hong (College of Pharmacy, Kangwon National University)
Oh, Joung-Weon (College of Pharmacy, Kangwon National University)
Asegahegn, Workaferhaw Shibru (College of Pharmacy, Kangwon National University)
Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University)
Publication Information
Archives of Pharmacal Research / v.29, no.11, 2006 , pp. 1061-1065 More about this Journal
Abstract
A new chiral derivatization agent with sugar moiety, 2,3,4,6-tetra-O-acetyl-${\beta}$-D-galactopyranosyl isothiocyanate (GATC) was synthesized. Several ${\beta}-blockers$ were investigated for the possible separation of the enantiomers by reversed-phase HPLC after derivatization with this new chiral derivatization agent (GATC). GATC was reacted readily with ${\beta}-blockers$ at room temperature and the reaction mixture could directly be injected into the HPLC system. The corresponding diastereomers were well resolved on an ODS column with acetonitrile-ammonium acetate buffer as a mobile phase and monitored at UV 254 nm. The optimization of the derivatization procedure (concentration of GATC, reaction temperature and time) and HPLC conditions (pH and ionic strength of mobile phase) were investigated and compared with GITC.
Keywords
Chiral separation; Chiral derivatization agent; ${\beta}-blockers$; HPLC;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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