Browse > Article

Torilin from Torilis japonica (Houtt.) DC. Blocks hKv1.5 Channel Current  

Kwak, Yong-Geun (Department of Pharmacology, Chonbuk National University Medical School)
Kim, Dae-Keun (College of Pharmacy, Woosuk University)
Ma, Tian-Ze (Department of Pharmacology, Chonbuk National University Medical School)
Park, Sun-Ah (Department of Pharmacology, Chonbuk National University Medical School)
Park, Hoon (College of Pharmacy, Woosuk University)
Jung, Young-Hoon (College of Pharmacy, SungKyunKwan University)
Yoo, Dong-Jin (Department of Chemistry, Seonam University)
Eun, Jae-Soon (College of Pharmacy, Woosuk University)
Publication Information
Archives of Pharmacal Research / v.29, no.10, 2006 , pp. 834-839 More about this Journal
Abstract
Torilin was purified from Torilis japonica (Houtt.) DC., and its effects on a rapidly activating delayed rectifier $K^+$ channel (hKv1.5), cloned from human heart and stably expressed in Ltk cells, as well as the corresponding $K^+$ current (the ultrarapid delayed rectifier, $I_{KUR}$) were assessed in human atrial myocytes. Using the whole cell configuration of the patch-clamp technique, torilin was found to inhibit the hKv1.5 current in time and voltage-dependent manners, with an $IC_50$ value of $2.51{\pm}0.34\;{\mu}M$ at +60 mV. Torilin accelerated the inactivation kinetics of the hKv1.5 channel, and slowed the deactivation kinetics of the hKv1.5 current, resulting in a tail crossover phenomenon. Additionally, torilin inhibited the hKv1.5 current in a use dependent manner. These results strongly suggest that torilin is a type of open-channel blocker of the hKv1.5 channel.
Keywords
hKv1.5 channel blocker; Torilin; Torilis japonca (Houtt.) DC;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Deal, K. K., England, S. K., and Tamkun, M. M., Molecular physiology of cardiac potassium channels. Physiol. Rev., 76, 49-67 (1996)   DOI
2 Eun, J. S., Park, J. A., Choi, B. H., Cho, S. K., Kim, D. K., and Kwak, Y. G., Effects of oxypeucedanin on hKv1.5 and action potential duration. Biol. Pharm. Bull., 28, 657-660 (2005a)   DOI   ScienceOn
3 Itokawa, H., Matsumoto, H., and Mihashi, S., Constituents of Torilis japonica D. C. I. Isolation and optical purity of germacra- 4(15), 5(E), 10(14)-trien-1a-ol. Chem. Pharm. Bull., 31, 1743- 1745 (1983a)   DOI
4 Itokawa, H., Matsumoto, H., and Mihashi, S., Isolation of oppositane- and cycloeudesmane-type sesquiterpenoids from Torilis japonica D. C. Chem. Lett., 1253-1256 (1983b)
5 Kang, S. S., Lee, E. B., Kim, T. H., Kim, K. R., and Jung, J. H., The NMR assignments of torilin from Torilis japonica. Arch. Pharm. Res., 17, 284-286 (1994)   DOI
6 Kim, M. S., Baek, J. H., Park, M. T., Sohn, T. K., Kim, S. E., Lee, J. J., and Kim, K. W., Anti-invasive activity of torilin, a sesquiterpene compound isolated from Torilis japonica. Oncol. Rep., 8, 359-364 (2001)
7 Wang, Z., Fermini, B., and Nattel, S., Sustained depolarizationinduced outward current in human atrial myocytes. Evidence for a novel delayed rectifier K+ current similar to Kv1.5 cloned channel currents. Circ. Res., 73, 1061-1076 (1993)   DOI   ScienceOn
8 Park, H. W., Choi, S. U., Baek, N. I., Kim, S. H., Eun, J. S., Yang, J. H., and Kim, D. K., Guaiane sesquiterpenoids from Torilis japonica and their cytotoxic effects on human cancer cell lines. Arch. Pharm. Res., 29, 131-134 (2006)   과학기술학회마을   DOI   ScienceOn
9 Ryu, J. H. and Jeong, Y. S., A new guaine type sesquiterpene from Torilis japonica. Arch. Pharm. Res., 24, 532-535 (2001)   DOI   ScienceOn
10 Tamkun M. M., Knoth, K. M., Walbridge, J. A., Kroemer, H., Roden, D. M., and Glover, D. M., Molecular cloning and characterization of two voltage-gated $K^+$ channel cDNAs from human ventricle. FASEB J., 5, 331-337 (1991)   DOI
11 Oh, H., Kim, J. S., Song, E. K., Cho, H., Kim, D. H., Park, S. E., Lee, H. S., and Kim, Y. C., Sesquiterpenes with hepatoprotective activity from Cnidium monnieri on tacrine-induced cytotoxicity in Hep G2 cells. Plant Med., 68, 748-749 (2002)   DOI   ScienceOn
12 Colatsky, T. J., Follmer, C. H., and Starmer, C. F., Channel specificity in antiarrhythmic drug action. Mechanism of potassium channel block and its role in suppressing and aggravating cardiac arrhythmias. Circulation, 82, 2235-2242 (1990)   DOI   ScienceOn
13 Kwak, Y. G., Navarro-Polanco, R. A., Grobaski, T., Gallagher, D. J., and Tamkun, M. M., Phosphorylation is required for alteration of Kv1.5 K+ channel function by the Kvbeta 1.3 subunit. J. Biol. Chem., 274, 25355-25361 (1999)   DOI
14 Sung, C. K., Kimura, T., Paul P. H., and Guo, J. X., International collation of traditional and folk medicine: Vol. 3. World scientific, Singapore, pp. 101-102, (1998)
15 Snyders, D. J., Tamkun, M. M., and Bennett, P. B., A rapidly activating and slowly inactivating potassium channel cloned from human heart. Functional analysis after stable mammalian cell culture expression. J. Gen. Physiol., 101, 513-543 (1993)   DOI   ScienceOn
16 Roden, D. M., Current status of class III antiarrhythmic drug therapy. Am. J. Cardiol., 72, 44B-49B (1993)   DOI   ScienceOn
17 Wang, Z., Fermini, B., and Nattel, S., Rapid and slow components of delayed rectifier current in human atrial myocytes. Cardiovasc. Res., 28, 1540-1546 (1994)   DOI   ScienceOn
18 Jeck, C. D. and Boyden, P. A., Age-related appearance of outward currents may contribute to development differences in ventricular repolarization. Circ. Res., 71, 1390-1403 (1992)   DOI   ScienceOn
19 Kitajima, J., Suzuki, N., Satoh, N., and Watanabe, M., Sesquiterpenoids of Torilis japonica fruit. Phytochemistry, 59, 811- 815 (2002)   DOI   ScienceOn
20 Lee, W. T., Coloured standard illustrations of Korean plants: Academy, Seoul, p. 263, (1996)
21 Eun, J. S., Choi, B. H., Park, J. A., Lee, G. I., Lee, T. Y., Kim, D. K. Jung, Y. H., Yoo, D. J., and Kwak, Y. G., Open channel block of hKv1.5 by psoralen from Heracleum moellendorffii Hance. Arch. Pharm. Res., 28, 269-273 (2005b)   과학기술학회마을   DOI   ScienceOn
22 Overturf, K. E., Russell, S. N., Carl, A., Vogalis, F., Hart, P. J., Humen, J. R., Sanders, K. M., and Horowitz, B., Cloning and characterization of a Kv1.5 delayed rectifier K+ channel from vascular and visceral smooth muscles. Am. J. Physiol., 267, C1231-C1238 (1994)   DOI
23 Kitajima, J., Suzuki, N., and Tanaka, Y., Guaiane-type sesquiterpenoid glycosides from Torilis japonica. Chem. Pharm. Bull., 46, 1743-1747 (1998)   DOI   ScienceOn
24 Lee, E. B., Cho, S. I., Kang, S. S., Kim, K. R., and Kim, T. H., Isolation of torilin from Torilis japonica fruit and its analgesic and anti-inflammatory activities. Kor. J. Pharmacogn., 30, 137-144 (1999)
25 Itokawa, H., Matsumoto, H., Mizuno, K., Watanabe, K., and Morita, H., Structures of torilolide and oxytorilolide; novel germacranolides from Torilis japonica (Houtt.) D. C. Chem. Pharm. Bull., 34, 4682-4686 (1986)   DOI
26 Snyders, D. J., Knoth, K. M., Roberts, S. L., and Tamkun, M. M., Time-, voltage, and state-dependent block by quinidine of a cloned human cardiac potassium channel. Mol. Pharmacol., 41, 322-330 (1992)
27 Choe, H., Lee, Y. K., Lee, Y. T., Choe, H., Ko, S. H., Joo, C. U., Kim, M. H., Kim, G. S., Eun, J. S., Kim, J. H., Chae, S. W., and Kwak, Y. G., Papaverine blocks hKv1.5 channel current and human atrial ultrarapid delayed rectifier $K^+$ currents. J. Pharm. Exp. Ther., 304, 706-712 (2003)   DOI   ScienceOn
28 Feng, J., Wible, B., Li, G. R., Wang, Z., and Nattel, S., Antisense oligodeoxynucleotides directed against Kv1.5 mRNA specifically inhibit ultrarapid delayed rectifier $K^+$ current in cultured adult human atrial myocytes. Circ. Res., 80, 572-579 (1997)   DOI   ScienceOn
29 Grissner, S., Potassium channels still hot. TiPS, 18, 347-350 (1997)   DOI   ScienceOn
30 Kim, M. S., Lee, Y. M., Moon, E-J., Kim, S. E., Lee, J. J., and Kim, K. W., Anti-angiogenic activity of torilin, a sesquiterpene compound isolated from Torilis japonica. Int. J. Cancer, 87, 269-275 (2000)   DOI   ScienceOn
31 Park, W. S., Son, E. D., Nam, G. W., Kim, S. H., Noh, M. S., Lee, B. G., Jang, J. S., Kim, S. E., Lee, J. J., and Lee, C. H., Torilin from Torilis japonica, as a new inhibitor of testosterone 5á-reductase. Planta Med., 69, 459-461 (2003)   DOI   ScienceOn