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Mutant Presenilin 2 Increases Acetylcholinesterase Activity in Neuronal Cells  

Nguyen Hong Nga (College of Pharmacy, Chungbuk National University)
Hwang Dae Youn (National Institute of Toxicological Research, Korea Food and Drug Administration)
Kim Young Kyu (National Institute of Toxicological Research, Korea Food and Drug Administration)
Yoon Do Young (Lab of Cell Biology, Korea Research Institute of Bioscience and Biotechnology)
Kim Jae Hwa (Lab of Cell Biology, Korea Research Institute of Bioscience and Biotechnology)
Lee Moon Soon (National Institute of Environmental Research)
Lee Myung Koo (College of Pharmacy, Chungbuk National University)
Yun Yeo Pyo (College of Pharmacy, Chungbuk National University)
Oh Ki Wan (College of Pharmacy, Chungbuk National University)
Hong Jin Tae (College of Pharmacy, Chungbuk National University)
Publication Information
Archives of Pharmacal Research / v.28, no.9, 2005 , pp. 1073-1078 More about this Journal
Abstract
A presenilin 2 mutation is believed to be involved in the development of Alzheimer's disease. In addition, transgenic mice with a presenilin 2 mutation have been reported to have learning and memory impairments. In this study, exposing PC12 cells expressing mutant presenilin 2 to $50{\mu}M\;A{\beta}_{25-35},\;30mM$ L-glutamate and $50{\mu}M\;H_2O_2$ caused a significant increase in acetylcholine esterase activity. An in vivo study revealed high levels of this enzyme activity in the mutant presenilin 2 transgenic brains compared with the wild type presenilin 2 transgenic and non-transgenic samples. These results suggest that a mutant presenilin 2-induced neurodegeneration in Alzheimer's disease might be involved in the increase in acetylcholinesterase activity. These findings might help in the development of an appropriate therapeutic intervention targeting mutant presenilin 2-induced Alzheimer's disease.
Keywords
Neuronal cell; Presenilin 2; Acetylcholine esterase; Alzheimer's disease;
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