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Comparison of Spectrophotometric, HPLC and Chemilumines­cence Methods for 3-Nitrotyrosine and Peroxynitrite Interaction  

Turan Nilufer Nermin (Gazi University, Faculty of Pharmacy, Department of Pharmacology)
Ark Mustafa (University of Mersin, Faculty of Medicine, Department of Pharmacology)
Demiryurek Abdullah Tuncay (University of Gaziantep, Faculty of Medicine, Department of Pharmacology)
Publication Information
Archives of Pharmacal Research / v.28, no.3, 2005 , pp. 358-363 More about this Journal
Abstract
We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration­dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCI) was also studied via the chemilumines-cence assay, where the HOCI-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.
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