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Manassantin A and B Isolated from Saururus chinensis Inhibit $TNF-{\alpha}-Induced$ Cell Adhesion Molecule Expression of Human Umbilical Vein Endothelial Cells  

Kwon Oh Eok (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Lee Hyun Sun (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Lee Seung Woong (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Chung Mi Yeon (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Bae Ki Hwan (College of Pharmacy, Chungnam National University)
Rho Mun-Chual (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Kim Young-kook (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Archives of Pharmacal Research / v.28, no.1, 2005 , pp. 55-60 More about this Journal
Abstract
Leukocyte adhesion to the vascular endothelium is a critical initiating step in inflammation and atherosclerosis. We have herein studied the effect of manassantin A (1) and S (2), dineolignans, on interaction of THP-1 monocytic cells and human umbilical vein endothelial cells (HUVEC) and expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in HUVEC. When HUVEC were pretreated with 1 and 2 followed by stimulation with $TNF-{\alpha}$, adhesion of THP-1 cells to HUVEC decreased in dose-dependent manner with $IC_{50}$ values of 5 ng/mL and 7 ng/mL, respectively, without cytotoxicity. Also, 1 and 2 inhibited $TNF-{\alpha}-induceda$ up-regulation of ICAM-1, VCAM-1 and E-selectin. The present findings suggest that 1 and 2 prevent monocyte adhesion to HUVEC through the inhibition of ICAM-1, VCAM-1 and E-selectin expression stimulated by $TNF-\alpha$, and may imply their usefulness for the prevention of atherosclerosis relevant to endothelial activation.
Keywords
Manassantin A/B; Cell adhesion molecules; Monocyte; Human umbilical vein endothelial cells; Atherosclerosis;
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