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Hepatotoxic Effect of 1-Bromopropane and Its Conjugation with Glutathione in Male ICR Mice  

Lee Sang Kyu (College of Pharmacy, Yeungnam University)
Jo Sang Wook (College of Pharmacy, Yeungnam University)
Jeon Tae Won (College of Pharmacy, Yeungnam University)
Jun In Hye (College of Pharmacy, Yeungnam University)
Jin Chun Hua (College of Pharmacy, Yeungnam University)
Kim Ghee Hwan (College of Pharmacy, Yeungnam University)
Lee Dong Ju (College of Pharmacy, Yeungnam University)
Kim Tae-Oh (School of Civil and Environmental Engineering, Kumoh National Institute of Technology)
Lee Eung-Seok (College of Pharmacy, Yeungnam University)
Jeong Tae Cheon (College of Pharmacy, Yeungnam University)
Publication Information
Archives of Pharmacal Research / v.28, no.10, 2005 , pp. 1177-1182 More about this Journal
Abstract
The hepatotoxic effects of 1-bromopropane (1-BP) and its conjugation with glutathione were investigated in male ICR mice. A single dose (1000 mg/kg, po) of 1-BP in corn oil to mice significantly increased serum activities of alanine aminotransferase and aspartate aminotransferase. Glutathione (GSH) content was dose-dependently reduced in liver homogenates 12 h after 1-BP treatment. In addition, 1-BP treatment dose-dependently increased levels of S-pro-pyl GSH conjugate at 12 h after treatment, as measured by liquid chromatography-electro-spray ionization tandem mass spectrometry. The GSH conjugate was maximally increased in liver at 6 h after 1-BP treatment (1000 mg/kg), with a parallel depletion of hepatic GSH content. Finally, 1-BP induced the production of malondialdehyde in liver. The present results suggest that 1-BP might cause hepatotoxicity, including lipid peroxidation via the depletion of GSH, due to the formation of GSH conjugates in male ICR mice.
Keywords
1-Bromopropane; Hepatotoxicity; Glutathione conjugates; LC/ESI-MS;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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