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Neuroprotective Effect of Wogonin： Potential Roles of Inflammatory Cytokines  

Piao, Hua-Zi (Department of Pharmacology, College of Medicine, Ewha Institute of Neuroscience, Ewha Womens University, Department of pharmacology, College of Medicine, Yanbian University)
Jin, Shun-Ai (Yan-Ji Hospital)
Chun, Hyang-Sook (Korea Food Research Institute)
Lee, Jae-Chul (Department of Pharmacology, College of Medicine, Ewha Institute of Neuroscience, Ewha Womens University)
Kim, Won-Ki (Department of Pharmacology, College of Medicine, Ewha Institute of Neuroscience, Ewha Womens University)
Publication Information
Archives of Pharmacal Research / v.27, no.9, 2004 , pp. 930-936 More about this Journal
Abstract
Wogonin (5,7-dihydroxy-8-methoxyflavone), an active component originated from the root of Scutellaria baicalensis Georgi, has been reported to possess antioxidant and anti-inflamma-tory properties. In this study, we investigated the neuroprotective effect of wogonin in a focal cerebral ischemia rat model. Wogonin markedly reduced the infarct volume after 2 h middle cerebral artery occlusion followed by 22 h reperfusion. Wogonin decreased the production of nitric oxide and inflammatory cytokines such as TNF-$\alpha$ and IL-6 in lipopolisaccharide-stimu-lated microglial cells. While wogonin reduced the activity of NF-$textsc{k}$B, it did not change the activ-ity of mitogen-activated protein kinases family members, p38, ERK and JNK. The lipopolisaccharide-stimulated production of NO and cytokines was significantly blocked by vari-ous kinds of NF-$textsc{k}$B inhibitors such as N-acetyl cysteine, pyrrolidinedithiocarbamate and MG-132. The data may indicate that wogonin has neuroprotective effect by preventing the over-activation of microglial cells, possibly by inactivating NF-$textsc{k}$B signaling pathway
Keywords
Wogonin; NF-${\kappa}B$; Microglia; Cytokine; Neuroprotection;
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