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Immobilized ${\beta}-Cyclodextrin$ as a Simple and Recyclable Method for Cholesterol Removal in Milk  

Kwak, H.-S. (Department of Food Science and Technology, Sejong University)
Kim, S.-H. (Department of Food Science and Technology, Sejong University)
Kim, J.-H. (Department of Applied Chemistry, Sejong University)
Choi, H.-J. (Department of Food Science and Technology, Sejong University)
Kang, J. (Department of Applied Chemistry, Sejong University)
Publication Information
Archives of Pharmacal Research / v.27, no.8, 2004 , pp. 873-877 More about this Journal
Abstract
This study was designed to determine the optimum conditions of three different factors (mixing time, mixing temperature, and tube size) in reduction of cholesterol in milk using immobilized $\beta$-CD beads. Immobilized $\beta$-CD glass beads were prepared at different conditions of silaniza-tion and $\beta$-CD immobilization reactions. In result, the glass beads (diameter 1 mm) at 20 mM 3-isocyanatopropyltriethoxysilane and 30 mM $\beta$-CD without base showed the highest choles-terol removal rate as 41%. Using above immobilized $\beta$-CD glass beads, the cholesterol removal rate was 40.2% with 6 h of mixing time in 7 mm diameter tube at $10^{\circ}C$. After choles-terol removal from milk, the glass beads were washed for cholesterol dissociation and reused. In recycling study, the cholesterol removal rate was 41%, which was mostly same as that using new glass beads. These results indicated that cholesterol removal rate was about 40% with $\beta$-CD immobilized glass beads, however, the recycling efficiency was almost 100%.
Keywords
${\beta}-CD$ immobilization; Cholesterol removal; Recycling;
Citations & Related Records

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1 Kwak, H. S., Chung, C. S., and Ahn, J., Flavor compounds of cholesterol-reduced Cheddar cheese slurries. Asian-Aust. J, Anim. Sci., 15, 117-123 (2002)
2 Larsen, J. E. and Froning, G. W., Extraction and processing of various components from egg yolk. Poult. Sci., 60, 160-167 (1981)   DOI
3 Mentink, L., Serpelloni, M., and Roquette, F., Process of refining mixture obtained from treatments of fatty media with cyclodextrin and containing complexes of cyclodextrin mainly with lipophilic substances other than fatty acids. Canadian Patent, 90/01007 (1990)
4 Ong, D. P., Lee, H. K., and Li, S. F. Y., Supercritical fluid extraction and chromatography of cholesterol in food sample. J. Chromatogr., 515, 509-513 (1990)   DOI   ScienceOn
5 Pyorala, K., Dietary cholesterol in relation to plasma cholesterol and coronary heart disease. Am. J. Clin. Nutr., 45, 1176-1184 (1987)
6 Szejtli, J., Chemistry and separation of cyclodextrins. In Szejtli, J. (Ed.). Cyclodextrins and their inclusion complexes; Akademiai Kiado, Budapest, Hungary, pp 17-43 (1982)
7 SAS Users Guide, Statistics, Version 5; SAS Institute, Inc., Cary, NC (1985)
8 Szjetli, J., Cyclodextrin inclusion complexes. In Szejtli, J., (Ed.). Cyclodextrin technology, Kluwer Akademic Publishers: Dordrecht, The Netherlands, pp 79-170 (1988)
9 Takahashi, K., Hattori, K., and Toda, F., Monotosylated ${\alpha} $- and ${\beta}$-cyclodextrins prepared in an alkaline aqueous solution. Tetrahedron Lett., 25, 3331-3334 (1984)   DOI   ScienceOn
10 Lee, D. K., Ahn, J., and Kwak, H. S., Cholesterol removal from homogenized milk with ${\beta}$-cyclodextrin. J. Dairy Sci., 82, 2327-2330 (1999)   DOI   PUBMED   ScienceOn
11 Kwak, H. S., Nam, C. G., and Ahn, J., Low cholesterol Mozza-rella cheese obtained from homogenized and ${\beta}$-cyclodextrin-treated milk. Asian-Aust. J. Anim. Sci., 14, 268-275 (2001)
12 Adams, M. L., Sullivan, D. M., Smith, R. L., and Richter, E. F., Evaluation of direct saponification method for determination of cholesterol in meats. J. AOAC, 69, 844-846 (1986)
13 Cornwell, M. J., Huff, J. B., and Bieniarz, C., A One-step synthesis of cyclodextrin monoaldehydes. Tetrahydron Lett., 36, 8371-8374 (1995)   DOI   ScienceOn
14 Lee, J. S., Ustunol, Z., and Smith, D. M., Cholesterol removal from animal with cyclodextrin and derivatives. J. Dairy Sci., 76 (Suppl. 1), 143 (Abst.) (1993)
15 Sieber, R., Cholesterol removal from animal food-can it be justified? Fed. Dairy Res. Inst., Libefeld, Switzerland, pp 375-387 (1990)
16 Gurr, M. I., Dietary lipids and coronary disease: old evidence, new perspectives and progress. Lipid Res., 31, 195-243 (1992)   DOI   PUBMED   ScienceOn
17 Kwak, H. S., Suh, H. M., Ahn, J., and Kwon, H. J., Optimization of ${\beta}$-cyclodextrin recycling process for cholesterol removal in cream. Asian-Aust. J. Anim. Sci., 14, 548-552 (2001)
18 Micich, T J., Behaviors of polymer supported digitonin with cholesterol in the absence and presence of butter oil. J. Agric. Food Chem., 38, 1839-1843 (1990)   DOI
19 Oakenfull, D. G. and Sihdu, G. S., Cholesteorl reduction. International Patent, 91/11114 (1991)
20 Watanabe, K. H., Aihara, H., and Nakamura, R., Properties of the purified extracellularcholesterol oxidase from Rhodococcus Equi. No. 23. J. Agric. Food Chem., 37, 1178-1182 (1989)   DOI
21 Carleton, R. A., Finberg, L., Flora, J., Goodman, D. S., Grundy, S. M., Havas, S., Hunter, G. T., Kritchevsky, D., Lauer, R. M., Lueoker, R. V., Ramirez, A. G., Van Horn, L., Stason, W. B., and Stokes, J., Report of the expert panel on population strategies for blood cholesterol reduction. Circulation, 83, 2154-2232 (1991)   DOI   PUBMED   ScienceOn
22 Schroder, B. G. and Baer, R. J., Utilization of cholesterol-reduced milk fat in fluid milks. Food Technol., Nov., 145-148 (1990)
23 Weu Shieh, C. P., Timothy, B. P., and Allan, H. C., Cyclodextrin refining process. US Patent, 5,393, 880 (1995)
24 Nagamoto, S., Cyclodextrin-expanding the development of their functions and applications. Chem. Economy Eng. Rev., 17, 28-34 (1985)
25 Kwak, H. S., Jung, C. S., Seok, J. S., and Ahn, J., Cholesterol removal and flavor development in Cheddar cheese. Asian-Aust. J. Anim. Sci., 16, 409-416 (2003)
26 Durkley, W. L., Reducing fat in milk and dairy products by processing. J. Dairy Sci., 65, 454-458 (1982)   DOI
27 Ahn, J. and Kwak, H. S., Optimizing cholesterol removal in cream using ${\beta}$-cyclodextrin and response surface methodology. J. Food Sci., 64, 629-632 (1999)   DOI   ScienceOn
28 Grundy, S. M., Brheimer, D., Blackburn, H., Brown, W. V., Kwiterovich, P.O., Mattson, F., Schonfeld, G., and Weidman, W. H., Rationale of the diet-heart statement of the American Heart Association Report of the Nutrition Committee. Circulation, 65, 839A-854A (1982)   DOI   PUBMED   ScienceOn
29 Makoto, K., Akio, O., and Reijiro, S., Cholesterol removal from animal with cyclodextrin by inclusion. Japan Patent, 4, 168, 198 (1992)
30 Yen, G. C. and Tsui, L. T., Cholesterol removal from a lardmixture with ${\beta}$-cyclodextrin. J. Food Sci., 60, 561-564 (1995)   DOI   ScienceOn
31 Wen Shieh, A. H., Process for separating cyclodextrin from a complex. US Patent, 5,371,209 (1994)