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Ginsenoside Rh2 Induces Apoptosis via Activation of Caspase-1 and -3 and Up-Regulation of Bax in Human Neuroblastoma  

Kim, Young-Soak (KT&G Central Research Institute)
Jin, Sung-Ha (Korea and Unviersity of Pennsylvania, School of Medicine, Department of Pharmacology)
Publication Information
Archives of Pharmacal Research / v.27, no.8, 2004 , pp. 834-839 More about this Journal
Abstract
In human neuroblastoma SK-N-BE(2) cells undergoing apoptotic death induced by ginsenos-ide Rh2, a dammarane glycoside that was isolated from Panax ginseng C. A. Meyer, caspase-1 and caspase-3 were activated. The expression of Bax was increased in the cells treated with ginsenoside Rh2, whereas Bcl-2 expression was not altered. Treatment with caspase-1 inhibi-tor, Ac-YVAD-CMK, or caspase-3 inhibitor, Z-DEVD-FMK, partially inhibited ginsenoside Rh2-induced cell death but almost suppressed the cleavage of the 116 kDa PARP into a 85 kDa fragment. When the levels of p53 were examined in this process, p53 accumulated rapidly in the cells treated early with ginsenoside Rh2. These results suggest that activation of caspase-1 and -3 and the up-regulation of Bax are required in order for apoptotic death of SK-N-BE(2) cells to be induced by ginsenoside Rh2, and p53 plays an important role in the pathways to promote apoptosis.
Keywords
Ginsenoside Rh2; Apoptosis; Caspase-1; Caspase-3; Bax; Neuroblastoma;
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Times Cited By Web Of Science : 19  (Related Records In Web of Science)
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