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Cytotoxicity and DNA Topoisomerases Inhibitory Activity of Constituents from the Sclerotium of Poria cocos  

Li, Gao (College of Pharmacy, Yanbian University)
Xu, Ming-Lu (College of Pharmacy, Yeungnam University)
Lee, Chong-Soon (Department of Biochemistry, College of Science, Yeungnam University)
Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu)
Chang, Hyun-Wook (College of Pharmacy, Yeungnam University)
Son, Jong-Keun (College of Pharmacy, Yeungnam University)
Publication Information
Archives of Pharmacal Research / v.27, no.8, 2004 , pp. 829-833 More about this Journal
Abstract
The bioactivity-guided fractionation of the methylene chloride extract of the sclerotium of Poria cocos led to the isolation of (S)-(+)-turmerone (1), ergosterol peroxide (2), polyporenic acid C (3), dehydropachymic acid (4), pachymic acid (5), and tumulosic acid (6). Compounds 4-6 exhibited moderate cytotoxicities, with $IC_{50}$ values of 20.5, 29.1, and $10.4{\;}\mu\textrm{m}$, respectively, against a human colon carcinoma cell line. However, 3-6 not only showed inhibitory activities as potent as etoposide used as a positive control on DNA topoisomerase II (36.1, 36.2, 43.9 and 66.7% inhibition at a concentration of $20{\;}\mu\textrm{m}$, respectively), but also inhibition of DNA topoisomerase I (55.8, 60.7, 43.5, and 83.3% inhibition at a concentration of $100{\;}\mu\textrm{m}$, respec-tively).
Keywords
Poria cocos; Polyporaceae; Cytotoxicity; DNA topoisomerases I and II inhibition;
Citations & Related Records

Times Cited By Web Of Science : 15  (Related Records In Web of Science)
Times Cited By SCOPUS : 13
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