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Antiproliferative Effect of Trichostatin A and HC-Toxin in T47D Human Breast Cancer Cells  

Joung, Ki-Eun (College of Pharmacy, Ewha Womans University)
Kim, Dae-Kee (College of Pharmacy, Ewha Womans University)
Sheen, Yhun-Yhong (College of Pharmacy, Ewha Womans University)
Publication Information
Archives of Pharmacal Research / v.27, no.6, 2004 , pp. 640-645 More about this Journal
Abstract
Histone deacetylase inhibitors are new class of chemotherapeutic drugs able to induce tumor cell apoptosis and／or cell cycle arrest. Trichostatin A, an antifungal antibiotic, and HC-toxin are potent and specific inhibitors of histone deacetylase activity. In this study, we have examined the antiproliferative activities of trichostatin A and HC-toxin in estrogen receptor positive human breast cancer, T47D cells. Both trichostatin A and HC-toxin showed potent antiprolifer-ative efficacy and cell cycle arrest at $G_2/M$ in T47D human breast cancer cells in a dose-dependent manner. Trichostatin A caused potent apoptosis of T47D human breast cancer cells and trichostatin A-induced apoptosis might be involved in an increase of caspase-3／7 activity. HC-toxin evoked apoptosis of T47D cells and HC-toxin induced apoptosis might not be medi-ated through direct increase in caspase-3/7 activity. We have identified potent activities of anti-proliferation, apoptosis, and cell cycle arrest of trichostatin A and HC-toxin in estrogen receptor positive human breast cancer cell line T47D.
Keywords
T47D cell; Trichostatin A; HC-toxin; Histone deacetylase;
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