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Regulation of c-Fos and c-Jun Gene Expression by Lipopolysaccharide and Cytokines in Primary Cultured Astrocytes: Effect of PKA and PKC Pathways  

Suh Hong-Won (Department of Pharmacology College of Medicine and Institute of Natural Medicine, Hallym University)
Choi Seong-Soo (Department of Pharmacology College of Medicine and Institute of Natural Medicine, Hallym University)
Lee Jin-Koo (Department of Pharmacology College of Medicine and Institute of Natural Medicine, Hallym University)
Lee Han-Kyu (Department of Pharmacology College of Medicine and Institute of Natural Medicine, Hallym University)
Han Eun-Jung (Department of Pharmacology College of Medicine and Institute of Natural Medicine, Hallym University)
Lee Jongho (Department of Pharmacology College of Medicine and Institute of Natural Medicine, Hallym University)
Publication Information
Archives of Pharmacal Research / v.27, no.4, 2004 , pp. 396-401 More about this Journal
Abstract
The effects of lipopolysaccharide (LPS) and several cytokines or the c-fos and c-jun mRNA expression were examined in primary cultured astrocytes. Either LPS (500 ng/mL) or inter-feron-$\gamma$ (IFN-$\gamma$ 5 ng/mL) alone increased the level of c-fos mRNA (1 h). However, tumor necro-sis factor-$\alpha$ (TNF-$\alpha$; 10 ng/mL) or interleukin-4 (IL-1$\beta$: 5 ng/mL) alone showed no significant induction of the level of c-fos mRNA. TNF-$\alpha$ showed a potentiating effect in the regulation of LPS-induced c-fos mRNA expression, whereas LPS showed an inhibitory action against IFN-Y-induced c-fos mRNA expression. LPS, but not TNF-$\alpha$, IL-1$\beta$ and IFN-$\gamma$, increased the level of c-jun mRNA (1 h). TNF-$\alpha$ and IFN-$\gamma$ showed an inhibitory action against LPS-induced c-jun mRNA expression. Both phorbol 12-myristate 13-acetate (PMA; 2.5 mM) and forskolin (FSK, 5 mM) increased the c-fos and c-jun mRNA expressions. In addition, the level of c-fos mRNA was expressed in an antagonistic manner when LPS was combined with PMA. When LPS was co-treated with either PMA or FSK, it showed an additive interaction for the induction of c-jun mRNA expression. Our results suggest that LPS and cytokines may be actively involved in the regulation of c-fos and c-jun mRNA expressions in primary cultured astrocytes. Moreover, both the PKA and PKC pathways may regulate the LPS-induced c-fos and c-jun mRNA expressions in different ways.
Keywords
Lipopolysaccharide; Cytokines; PMA; Forskolin; c-Fos; c-Jun; Astrocytes;
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