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Prevalence and Characterization of Vancomycin-Resistant Enterococci in Chicken Intestinles and Humans of Korea  

Seong, Chi-Nam (Department of Biological Science, Sunchon National University)
Shim, Eun-Sook (Department of Biological Science, Sunchon National University)
Kim, Shin-Moo (Department of Clinical Pathology, Wonkwang Health Science College)
Yoo, Jin-Cheol (Department of Pharmacy, Chosun University)
Publication Information
Archives of Pharmacal Research / v.27, no.2, 2004 , pp. 246-253 More about this Journal
Abstract
The prevalence, genotype for antibiotic resistance and antibiotic susceptibility of vancomycin resistant enterococci (VRE) were determined. And molecular typings of the Enterococcus faecium isolates were analyzed. Prevalence of VRE in chickens, healthy children and intensive care unit (ICU) patients was 41.6％,7.9％, and 20.4％, respectively. Forty out of 54 isolates from chicken intestines, and 9 out of 11 from ICU patients were identified as Enterococcus faecium. Eleven out of 13 isolates from non-hospitalized young children were E. gallinarium. Twelve strains of E. faecalis were isolated from chicken intestines. The gene for the antibiotic resistance in E. faecium, and E. faecalis was vanA, while that in E. gallinarium was vanC1. E. faecium isolates were resistant to most of antibiotics except ampicillin and gentamicin. Molecular typing of the E. faecium strains obtained by pulse field gel electrophoresis and repetitive sequence-based PCR suggest that VRE transmit horizontally from poultry to humans, especially young children, via the food chains in Korea.
Keywords
Vancomycin resistant enterococci (VRE); Prevalence; Molecular typing; Chicken intestine; Enterococcus faecium; E. faecalis;
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