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Involvement of Nitric Oxide During In Vitro Fertilization and Early Embryonic Development in Mice  

Kim, Bo-Hyun (Eden Obstetric & Gynecology Clinic)
Kim, Chang-Hong (Eden Obstetric & Gynecology Clinic)
Jung, Kyu-Young (Department of Pharmacology, Wonkwang University School of Medicine)
Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Ju, Eun-Jin (Department of Biological science, College of Natural Sciences and Medicinal Resources Research Center, Wonkwang University)
Choo, Young-Kug (Department of Biological science, College of Natural Sciences and Medicinal Resources Research Center, Wonkwang University)
Publication Information
Archives of Pharmacal Research / v.27, no.1, 2004 , pp. 86-93 More about this Journal
Abstract
Nitric oxide (NO) has emerged as an important intracellular and intercellular messenger, controlling many physiological processes and participating in the fertilization process via the autocrine and paracrine mechanisms. This study investigated whether nitric oxide synthase (NOS) inhibitior (L-NAME) and L-arginine could regulate in vitro fertilization and early embryonic development in mice. Mouse epididymal spermatozoa, oocytes, and embryos were incubated in mediums of variable conditions with and without L-NAME or L-arginine (0.5, 1, 5 and 10mM). Fertilization rate and early embryonic development were significantly inhibited by treating sperms or oocytes with L-NAME (93.8% vs 66.3%,92.1% vs 60.3%), but not with L-arginine. In contrast, fertilization rate and early embryonic development were conspicuously reduced when L-NAME or L-arginine was added to the culture media for embryos. Early embryonic development was inhibited by microinjection of L-NAME into the fertilized embryosin a dose-dependent manner, but only by high concentrations of L-arginine. These results suggest that a moderate amount of NO production is essential for fertilization and early embryo development in mice.
Keywords
Early embryonic development; In vitro fertilization; Nitric oxide; L-NAME; L-Arginine;
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Times Cited By Web Of Science : 9  (Related Records In Web of Science)
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1 Barroso, R. P., Osuamkpe, C., Nagamani, M., and Yallampalli, C., Nitric oxide inhibits development of embryos and implantation in mice. Mol. Hum. Reprod., 4, 503-507 (1998)   DOI   ScienceOn
2 Biswas, S., Kabir, S., and Pal, A. K., The role of nitric oxide in the process of implantation in rats. J. Reprod. Fertil., 114, 157-161 (1998)   DOI   PUBMED
3 Baldi, E., Luconi, M., Bonaccorsi, L., Krausz, C., and Forti, G., Human sperm activation during capacitation and acrosome reaction: role of calcium, protein phosphorylation and lipid remodelling pathways. Front. Biosci., 1, d189-205 (1996)   DOI
4 Bredt, D. S. and Synder, S. H., Nitric oxide mediates gultamate-linked enhancement of cGMP levels in the cerebellum. Proc. Natl. Acad. USA, 86, 9030-9033 (1989)   DOI   ScienceOn
5 Garthwait, J., Charles, J, S., and Chess-Williams, R., Endothelium-derived relaxing factor release on activation of NMDA receptors suggests roles as intracellular messenger in the brain. Nature. London, 336, 385-388 (1988)   DOI   ScienceOn
6 Herrero, M. B., Goin, J. C., Boquet, M., Canteros, M. G., Franchi, A. M., Perez Martinez, S., Polak, J. M., Viggiano, J. M., and de Gimeno, M. A. F., The nitric oxide synthase of mouse spermatozoa. FEBS. Lett., 411, 39-42 (1997)   DOI   ScienceOn
7 Herrero, M. B., Perez Martinez, S., Viggiano, J. M., Polak, J. M., and de Gimeno, M. F., Localization by indirect immunofluorescence of nitric oxide synthase in mouse and human spermatozoa. Reprod. Fertil. Dev., 8, 931-934 (1996b)
8 Hellstrom, W. J. G., Bell, M., Wang. R., and Sikka, S. C., Effect of sodium nitroprusside on sperm motility, viability, and lipid peroxidation. Fertil. Steril., 61,1117-1122 (1994)   DOI   PUBMED
9 Jung, K. Y., Kim, B. H., Hwang, M. R., Cho, J. R., Kim, H. M., Lee, Y. C., Kim, C. H., Kim, J. K., and Choo, Y. K., Differential distribution of ganglioside GM3 in seminiferous tubule and epididymis of adult rats. Arch. Pharm. Res., 24, 360-366 (2001)   DOI   ScienceOn
10 Lewis, S. E. M., Donnelly, E. T., Sterling, E. S. L., Kennedy, M. S., Thompson, W., and Chakravarthy, U., Nitric oxide synthase and nitrite production in human spermatozoa: evidence that endogenous nitric oxide is beneficial to sperm motility. Mol. Hum. Reprod., 2, 873-878 (1996)   DOI   ScienceOn
11 Oh, S. H., Miyoshi, K., and Funahashi, H., Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage. Biol. Reprod., 59, 884-889 (1998)   DOI   ScienceOn
12 Sladek, S. M., Regenstein, A. C., Lykins, D., and Roberts, J. M., Nitric oxide synthase activity in pregnant rabbit uterus decreases on the last day of pregnancy. Am. J. Obstet. Gynecol., 160, 1285-1291 (1993)
13 Choi, H. G., Kim, J. K., Cho, J. R., Kim, B. J., Jung, K. Y., Choi, B. K., Shin, M. K., and Choo. Y. K., Effects of high molecular weight water-soluble chitosan on in vitro fertilization and ovulation in mice fed a high-fat diet. Arch. Pharm. Res., 25, 178-183 (2002)   DOI   ScienceOn
14 Herrero, M. B., Viggiano, J. M., Perez Martinez, S., and de Gimeno, M. A. F., Effects of nitric oxide synthase inhibitors on the outcome of in vitro fertilization in the mouse. Reprod. Fertil. Dev., 8, 301-314 (1996a)   DOI   ScienceOn
15 Schmidt, H. H. H. W., Lohmann, S. M., and Walter, U., The nitric oxide and cGMP signal transduction system: regulation and mechanism of action. Biochim. Biophys. Acta, 1178, 153-175 (1993)   DOI   PUBMED   ScienceOn
16 Toyoda, Y. and Chang, M. C., Fertilization of rat eggs in vitro by epididymal spermatozoa and the development of eggs following transfer. J. Reprod. Fertil., 36, 9-22 (1974)   DOI   PUBMED
17 Moncada, S., Palmer, R. M. J., and Higgs, E. A., Nitric oxide: physiology, pathophysiology and pharmacology. Pharmcol. Rev., 43, 109-142 (1991)   PUBMED
18 O'Bryan, M. K., Zini, A., Cheng, G. Y., and Schlegel, P. N., Human sperm endothelial nitric oxide synthase expression: correlation with sperm motility. Fertil. Steril., 70, 1143-1147 (1998)   DOI   ScienceOn
19 Revelli, A., Soldati, G., Costamagna, C., Pellerey,O., Aldieri, E., Massobrio, M., Bosia, A., and Ghigo, D., Follicular fluid proteins stimulate nitric oxide (NO) synthase in human sperm: a possible role for NO in acrosomal reaction. J. Cell Physiol., 178, 85-92 (1999)   DOI   ScienceOn
20 Kuo, R. C., Baxter, G. T., Thompson, S. H., Stricker, S. A., Patton, C., Bonaventura, J., and Epel, D., NO is necessary and sufficient for egg activation at fertilization. Nature, 406, 633-636 (2000)   DOI   ScienceOn
21 Gouge, R. C., Marshburn, P., Gordon, B. E., Nunley, w., and Huet-Hudson, Y. M., Nitric oxide as a regulator of embryonic development. Biol. Reprod., 58, 875-879 (1998)   DOI   ScienceOn
22 Nathan, C., Nitric oxide as a secretory product of mammalian cells. FASEB J., 6, 3051-3064 (1992)   DOI
23 Hibbs, J. B., Taintor, Jr. R. R., and Vavrin, Z., Macrophage cytotoxicity: role for L-arginine deminase and imino nitrogen oxidation to nitrite. Science. Wash. DC., 325, 473-476 (1987)
24 Gagiot, S., Scavone, C., and Bevilacqua, E., Participation of the mouse implanting trophoblast in nitric oxide production during pregnancy. Biol. Reprod., 62, 260-268 (2000)   DOI   ScienceOn
25 Zini, A., Lamirande, E. D., and Gagnon, C., Low levels of nitric oxide promote human sperm capacitation in vitro. J. Androl., 16, 424-431 (1995)   PUBMED
26 Burnett, A. L., Ricker, D. D., Chamness, S. L., Maguire, M. P., Crone, J. K., Bredt, D. S., Snyder, S. H., and Chang, T. S., Localization of nitric oxide synthase in the reproductive organs of the male rat. Biol. Reprod., 52, 1-7 (1995)   DOI   ScienceOn
27 Chen, H. W., Jiang, W. S., and Tzeng, C. R., Nitric oxide as a regulator in preimplantation embryo development and apoptosis. Fertil. Steril., 75,1163-1171 (2001)   DOI   ScienceOn
28 Nobunaga, T., Takugawa, Y., Hashimoto, K., Kubota, Y., Sawai, K., and Kimura, T., Elevated nitric oxide concentration in the seminal plasma of infertile males: nitric oxide inhibits sperm motility. Am. J. Rep. Immunol., 36, 197-197 (1996)
29 Hubbard, C. J. and Price, J., The effects of follicle-stimulating hormone and cyclic guanosine 3', 5'-monophosphate and cyclic adenosine 3', 5'-monophosphate-phosphodiesterase and resumption of meiosis in hamster cumulus-oocyte complexes. Biol. Reprod., 39,829-838 (1988)   DOI   ScienceOn
30 Sladex, S. M., Magness, R. R., and Conrad, K. P., Nitric oxide and pregnancy. Am. J. Physiol., 272, R441-R463 (1997)
31 Kwak, D. H., Jung, K. Y., Lee, Y. C., and Choo, Y. K., Expressional changes of ganglioside GM3 during ovarian maturation and early development in db/db mice. Develop. Growth. Differ., 45, 95-102 (2003)   DOI   ScienceOn
32 Rosselli, M., Dubey, R. K., Imthurn, B., Macas, E., and Keller, P., Effects of nitric oxide on human spermatozoa: evidence that nitric oxide decrease sperm motility and induces sperm toxicity. Hum. Reprod., 10, 1786-1790 (1995)   DOI
33 Adams, M. L., Nock, B., Trung, R., and Cicero, T. J., Nitric oxide control of steroidogenesis: endocrine effects of $N^{G}$-nitro-L-arginine and comparisons to alcohol. Life. Sci., 50, PL35-40 (1992)   DOI   ScienceOn
34 Ignarro, L. J., Buga, G. M., Wood, K. S., Byrns, R. E., and Chaudhuri, G., Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc. Natl. Acad. Sci. USA, 84, 9265-9269 (1987)   DOI   ScienceOn
35 Weinberg, J. B., Doty, E., Bonaventura, J., and Haney, A. F., Nitric oxide inhibition of human sperm motility. Fertil. Steril., 62,1244-1249 (1995)
36 Yeoman, R. R., Jones, W. D., and Rizk, B. M., Evidence for nitric oxide regulation of hamster sperm hyperactivation. J. Androl., 1, 58-64 (1998)
37 Dey, S. K., Kimura, F., Mukherjee, F., and Dickmann, Z. J., Cyclic-AMP and cyclic-GMP in rabbit blastocysts. Reprod. Fertil., 52, 235-237 (1978)   DOI   PUBMED
38 Izumi, Y., Clifford, D. B., and Zorumski, C. F., Inhibition of longterm potentiation by NMDA-mediated nitric oxide release. Science. Wash. DC., 257,1273-1276 (1992)   DOI   PUBMED