Browse > Article

Phospholipids from Bombycis corpus and Their Neurotrophic Effects  

Kwon, Hak-Cheol (College of Pharmacy, SungKyunKwan University)
Jung, I-Yeon (Department of Sericulture and Entomology, National Institute Agricultural Science and Technology, RDA)
Cho, Se-Yeon (Department of Sericulture and Entomology, National Institute Agricultural Science and Technology, RDA)
Cho, Ock-Ryun (College of Pharmacy, SungKyunKwan University)
Yang, Min-Cheol (College of Pharmacy, SungKyunKwan University)
Lee, Sung-Ok (College of Pharmacy, SungKyunKwan University)
Hur, Jin-Young (Korea and Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Sun-Yeou (Korea and Graduate School of East-West Medical Science, Kyung Hee University)
Yang, Jong-Beom (Dept. of Food Science ＆ Technology, DongNam Health College)
Lee, Kang-Ro (College of Pharmacy, SungKyunKwan University)
Publication Information
Archives of Pharmacal Research / v.26, no.6, 2003 , pp. 471-477 More about this Journal
Abstract
Three phospholipids (4-6) and three aromatic amines (1-3) were obtained from the methanol extract of Bombycis corpus. Based on spectral data, their structures have been elucidated as nicotiamide (1), cytidine (2), adenine (3), 1-Ο-(9Z-octadecenoyl)-2-Ο-(8Z,11Z-octadecadienoyl)-sn-glycero-3-phosphorylcholine (4), 1,2-di-Ο-hexadecanoyl-sn-glycero-3-phosphorylcholine (5) and 1,2-di-Ο-9Z-octadecenoyl-sn-glycero-3-phosphorylcholine (6). We examined the effects of compounds on synthesis of NGF in cultured astrocytes. By RT-PCR analysis, expresison of NGF mRNA in astrocytes cultured in serum-starvation increased after the addition of phospholipid (10 $\mu$M). The NGF content in the culture medium was significantly increased by compound 5, compared with the control value. These results suggest that three phospholipid compounds isolated from the methanol extract of Bombycis corpus may exert neurotrophic effects by stimulation of NGF synthesis in astrocytes.
Keywords
Bombycis corpus; Phospholipid; Diacylglycerophosphorylcholines; Neurotrophic effect;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Cheng, K. P., Nagano, H., Bang, L., Ourisson, G., and Beck, J. P., Chemistry and biochemistry of Chinese drugs. Part I. Sterol derivatives cytotoxic to hepatoma cells, isolated from the drug Bombyx cum Botryte. J. Chem. Res. (S), 217 (1977)
2 Gamo, S., Kawabe, A., Kohara, H., Yamaguchi, H., Tanaka, Y., and Yagi, S. Fast atom bombardment tandem mass spectrometric analysis of phospholipids in Drosophila melanogaster. J. Mass Spectrom, 34, 590-600 (1999)   DOI   ScienceOn
3 Ishihara, M. and Sano, A., Facile and useful synthsis of enantiomeric phosphatidylcholines. Chem. Pharm. Bull., 44, 1096-1098 (1996)   DOI   ScienceOn
4 Pemberton, R. W., Insects and other arthropods used as drugs in Korean traditional medicine. J. Ethnopharmacol., 65, 207-216 (1999)   DOI   PUBMED   ScienceOn
5 Singleton, J. A., Ruan, M., Sanford, J. H., Haney, C. A., and Stikeleather, L. F. Separation and characterization of peanut phospholipid molecular species using high-performance liquid chromatography and fast atom bombardment mass spectrometry. J. Am. Oil Chem. Soc., 76, 49-56 (1999)   DOI   ScienceOn
6 Pouchert, C. J. and Behnke, J. (Ed.), The Aldrich Library of $^{13}C$ and $^{1}H$ FT NMR Spectra, Aldrich Chemical Company, Inc., USA, Vol. 3, p 388 (1993b)
7 Sekiya, M. and Suzuki, J., Facile Synthesis of Hypoxanthine and Adenine. Chem. Pharm. Bull., 20, 209-210 (1972)   DOI
8 Shanghai Science and Technologic Publisher and Shougakukan, The Dictionary of Chinese Drugs, Shougakukan, Tokyo, pp.2238-2240 (1985)
9 Pouchert, C. J. and Behnke, J. (Ed.), The Aldrich Library of $^{13}C$ and $^{1}H$ FT NMR Spectra, Aldrich Chemical Company, Inc., USA, vol. 3, p 338 (1993a)
10 Fallani, A., Tombaccini, D., and Ruggieri, S. Structural analysis of phosphatidylcholines of Yoshida ascites hepatoma and liver cells from host rats fed a control and an essential fatty acid-deficient diet. Biochim. Biophys. Acta, 450, 210-224 (1976)   DOI   PUBMED   ScienceOn
11 Kwon, H. C., Zee, O. P., and Lee, K. R., Two New Monogalactosyl-glycerols from Hydrocotyle ramiflora. Planta Med., 64, 477-479 (1998)   DOI   ScienceOn
12 Tohda, C., Nakamura, N., Komatsu, K., and Hattori, M., Trigonelline-induced Neurite Outgrowth in human neuroblastoma SK-N-SH cells. Biol. Pharm. Bull., 22, 679-682 (1999)   DOI   PUBMED   ScienceOn
13 Batrakov, S. G., Konova, I. V., Sheichenko, V. I., Esipov, S. E., and Galanina, L. A., Two unusual glycerophospholipids from a filamentous fungus, Absidia corymbifera. Biochim. Biophys. Acta, 1531, 169-177 (2001)   DOI   PUBMED   ScienceOn
14 Arora, A. and Gupta, C. M., Glycerol backbone conformation in phosphatidylcholines is primarily determined by the intramolecular stacking of the vicinally arranged acyl chains. Biochim. Biophys. Acta, 1324, 47-60 (1997)   DOI   ScienceOn
15 Woodruff, R. H. and Franklin, R. J., Growth factors and remyelination in the CNS. Histol. Histopathol., 12, 459-466 (1997);Chemical Abstracts, vol.127, 60699   PUBMED
16 Jung, J. H., Lee, H. K., and Kang, S. S., Diacylglycerylgalactosides from Arisaema amurense. Phytochemistry, 42, 447-452 (1996)   DOI   ScienceOn
17 Solodin, I., Brown, C. S., and Heath, T. D., Synthesis of phosphotriester cationic pospholipids. Cationic lipids 2. Synlett., 457-458 (1996);Chemical Abstracts, vol.125, 221165
18 Limb, J.-K., Kim, Y. H., Han, S.-Y., and Jhon, G.-J., Isolation and Characterization of monoacetyldiglycerides from bovine udder. J. Lipid Res., 40, 2169-2176 (1999)   PUBMED