Browse > Article

Effects of Polycyclic Aromatic Hydrocarbons on Liver and Lung Cytochrome P450s in Mice  

Kim, Ji-Young (College of Pharmacy, Yeungnam University)
Lee, Sang-Kyu (College of Pharmacy, Yeungnam University)
Kim, Chun-Hwa (College of Pharmacy, Yeungnam University)
Jeon, Tae-Won (College of Pharmacy, Yeungnam University)
Moon, Chang-Kiu (College of Pharmacy, Seoul National University)
Lee, Hye-Sook (College of Pharmacy, Wonkwang University)
Yoo, Sun-Dong (College of Pharmacy, Sungkyunkwan University)
Lee, Eung-Seok (College of Pharmacy, Yeungnam University)
Jeong, Tae-Cheon (College of Pharmacy, Yeungnam University)
Publication Information
Archives of Pharmacal Research / v.26, no.5, 2003 , pp. 394-404 More about this Journal
Abstract
Certain polycyclic aromatic hydrocarbons (PAHs) have been reported to induce cytochrome P450 (CYP) 1A1 and 1A2. In the present study, the effects of six well-known PAHs, such as benzo[a]pyrene, benz[a]anthracene, dibenz[a,h]anthracene, chrysene, benzo[k]fluorancene and benzo[b]fluorancene, on the activities of hepatic and pulmonary CYP enzymes were investigated in male ICR mice. When mice were treated intraperitoneally with 3, 10 and 30 mg/kg of individual PAHs for 3 consecutive days, the activities of ethoxyresorufin- and methoxyresorufin-Ο-dealkylases were significantly and differentially induced in both liver and lung. Moreover, other CYP isozyme-associated monooxygenase activities were also induced significantly in liver and lung with characteristic induction profiles. Our present results suggest that individual PAHs might have inductive effects on CYP isozymes, and that the characteristic inductive effects of individual PAHs on certain CYP isozymes would be developed as a marker for determining exposure to certain PAHs.
Keywords
Polycyclic aromatic hydrocarbons; Cytochrome P450s; Induction; Liver; Lung; Mice;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Machala, M., Matlova, L., Svoboda, I., and Nezveda, K., Induction effects of polychlorinated biphenyls, polycyclic aromatic hydrocarbons and other widespread aromatic environmental pollutants on microsomal monooxygenase activities in chick embryo liver. Arch. Toxicol., 70, 362-367 (1996)   DOI   ScienceOn
2 Nash, T., The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem. J., 55, 416-421 (1953)   DOI   PUBMED
3 Villeneuve, D. L., Crunkilton, R. L., and DeVita, W. M., Identification of cytochrome P4501A inducers in complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Society of Environmental Toxicology and Chemistry, Second World Congress, Vancouver, Canada, Abstract No. PT215 (1995)
4 Buckly, T. J. and Lioy, P. J., An examination of the time course from human dietary exposure to polycyclic aromatic hydrocarbons to urinary elimination of 1-hydroxypyrene. Br. J. Ind. Med., 49, 113-124 (1992)   PUBMED
5 Guengerich, F. P. and Shimada, T., Activation of procarcinogens by human cytochrome P450 enzymes. Mutation Res., 400, 201-213 (1998)   DOI   ScienceOn
6 Guengerich, F. P. and Shimada, T., Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes. Chem. Res. Toxicol., 4, 391-407 (1991)   DOI   ScienceOn
7 Heidelberger, C., Chemical carcinogenesis. Annu. Rev. Biochem., 44, 79-121 (1975)   DOI   PUBMED   ScienceOn
8 IARC, Polynuclear aromatic compounds, IARC Monographs on the Evaluation of the Carcinogenic Risks of Chemical to Humans, 32, 355-364, World Health Organization, Lyon, France (1983)
9 Kielhorn, J. and Boehnicke, A., Guidelines for drinking water quality. Addendum to vol. 2 Health criteria and other supporting information, 2nd edition. World Health Organization, Geneva, 123-152 (1998)
10 Lowry, O. H., Rosebrough, N. H., Farr, A. L. and Randall, R. J., Protein measurement with folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951)   PUBMED
11 Guengerich, F. P., Human cytochrome P450 enzymes, in: P.R. Ortiz de Mentellano (Ed.), Cytochrome P450, Plenum Press, New York, 473-535 (1995)
12 Dipple, A., Michejda, C. J., and Weisburger, E. M., Metabolism of chemical carcinogens. Pharmacol. Ther., 27, 265-296 (1985)   DOI   ScienceOn
13 Nisbet, I. C. T. and LaGoy, P. K., Toxic equivalency factors (TEFs) for polycyclic aromatic hydrocarbons (PAHs). Reg. Toxicol. Pharmacol., 16, 290-300 (1992)   DOI   ScienceOn
14 Brunstrom, B., Broman, D., and Naf, C., Toxicity and EROD-inducing potency of 24 polycyclic aromatic hydrocarbons (PAHs) in chick embryos. Arch. Toxicol., 65, 485-489 (1991)   DOI   ScienceOn
15 Petry, T., Schmid, P., and Schlatter, C., The use of toxic equivalency factors in assessing occupational and environmental health risk associated with exposure to airborne mixtures of polycyclic aromatic hydrocarbons (PAHs). Chemosphere, 32, 639-648 (1996)   DOI   ScienceOn
16 Menzie, C. A., Potocki, B. B., and Santodonato, J., Exposure to carcinogenic PAHs in the environment. Environ. Sci. Technol., 26, 1278-1284 (1992)   DOI
17 Conney, A. H., Induction of microsomal enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons: G. H. A. Clowes Memorial Lecture. Cancer Res., 42, 4875-4917 (1982)   PUBMED
18 United States Environmental Protection Agency (USEPA), Provisional Guidance for Quantitative Risk Assessment of Polycyclic Aromatic Hydrocarbons, EPA/600/R-93/089. United States Environmental Protenction Agency (1993)
19 Blank, J. A., Tucker, A. N., Sweatlock, J., Gasiewicz, T. A., and Luster, M. I., $\alpha$-Naphthoflavone antagonism of 2,3,7,8-tetrachlorodibenzo-p-dioxin induced murine ethoxyresorufin O-deethylase activity and immunosuppression. Mol. Pharmacol., 32, 168-172 (1987)
20 Gelboin, H. V., Benzo[a]pyrene metabolism, activation, and carcinogenesis: role and regulation of mixed function oxidases and related enzymes. Physiol. Rev., 60, 1107-1166 (1980)   DOI   PUBMED
21 Willett, K. L., Gardinali, P. R., Sericano, J. L., Wade, T. L., and Safe, S. H., Characterization of the H4IIE rat hepatoma cell bioassay for evaluation of environmental samples containing polynuclear aromatic hydrocarbons (PAHs). Arch. Environ. Contam. Toxicol., 32, 442-448 (1997)   DOI   ScienceOn
22 Koop, D. R., Hydroxylation of p-nitrophenol by rabbit ethanolinducible cytochrome P450 isozyme 3a. Mol. Pharmacol., 29, 399-404 (1986)   PUBMED
23 Conney, A. H., Chang, R. L. Jerina, D. M., and Wei, S. J., Studies on the metabolism of benzo[a]pyrene and dosedependent difference in the mutagenic profile of its ultimate carcinogenic metabolite. Drug Metab. Rev., 26 125-163 (1994)   DOI   ScienceOn
24 White, K. L., Jr., Lysy, H. H., and Holsapple, M. P., Immunosuppression by polycyclic aromatic hydrocarbons: a structureactivity relationship in B6C3F1 and DBA/2 mice. Immunopharmacology, 9, 155-164 (1985)   DOI   ScienceOn
25 Lubet, R. A., Meyer, R. T., Cameron, J. W., Nims, R. W., Burke, M. D., Wolff, J., and Guengerich, F. P. Dealkylation of pentoxyresorufin: a rapid and sensitive assay for measuring induction of cytochrome(s) P-450 by phenobarbital and other xenobiotics in the rat. Arch. Biochem. Biophys., 238, 43-48 (1985)   DOI   ScienceOn
26 Guengerich, F. P., Metabolism of chemical carcinogens. Carcinogenesis, 21, 345-351 (2000)   DOI   PUBMED   ScienceOn