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The Polysaccharide Fraction AIP1 from Artemisia iwayomogi Suppresses Apoptotic Death of the Mouse Spleen Cells in Culture  

Hwang, Jung-Suk (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Chung, Hye-Kyung (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Bae, Eun-Kyong (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Lee, Ah-Young (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Ji, Hee-Jung (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Park, Dong-Woon (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Jung, Hwa-Jin (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Cho, Chung-Won (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University)
Choi, Hyun-Ju (Department of Medical Technology, Inje University)
Lee, Dong-Seok (Deaprtment of Medical Technology, Inje University)
Lee, Kang-Ro (Deaprtment of Medical Technology, Inje University)
Youn, Hyun-Joo (Deaprtment of Medical Technology, Inje University)
Publication Information
Archives of Pharmacal Research / v.26, no.4, 2003 , pp. 294-300 More about this Journal
Abstract
A polysaccharide fraction, AIP1, purified from Artemisia iwayomogi was shown to have immunomodulating and anti-tumor activities in mice. In order to determine how the AIP1 fraction exhibits the immunomodulating activity, the effect of the fraction on the apoptosis of mouse spleen cells was investigated. Treatment of the mouse spleen cells with the AIP1 fraction resulted ,in the suppression of apoptotic death and an extension of cell survival in culture, indicating that the fraction might modulate the death of spleen cells. Treatment of the mice with the AIP1 fraction in vivo also resulted in less apoptosis of the spleen cells, which indicates the physiological relevance of the anti-apoptosis effect of the fraction in vitro. A mouse gene array was used to determine the profile of the gene expression change showing a pattern of up- and down-regulated genes by the AIP1 treatment. This study provides preliminary information regarding the immunomodulatory mechanism of the AIP1 fraction.
Keywords
Polysaccharide; Artemisia iwayomogi; Apoptosis; Genomics;
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1 Blagosklonny, M. V., Hsp-90-associated oncoproteins: multiple targets of geldanamycin and its analogs. Leukemia, 16, 455-462 (2002)   DOI   PUBMED   ScienceOn
2 Dupuis, M., Denis-Mize, K., LaBarbara, A., Peters, W., Charo., I. F., McDonald, D. M., and Ott, G., Immunization with the adjuvant MF59 induces macrophage trafficking and apoptosis. Eur. J. Immunol., 31, 2910-2918 (2001)   DOI   ScienceOn
3 Emelyanov, A. V., Kovac, C. R., Sepulveda, M. A., and Birshtein, B. K., The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells. J. Biol. Chem., 277, 11156-11164 (2002)   DOI   ScienceOn
4 Iihara, K., Sasahara, M., Hashimoto, N., and Hazama, F., Induction of platelet-derived growth factor beta-receptor in focal ischemia of rat brain. J. Cereb. Blood. Flow. Metab., 16, 941-949 (1996)   PUBMED
5 Mishell, B. B. and Shiigi S. M., 'Seleced methods in cellular immunology' W. H. Freeman and Company, New York, (1980)
6 Rudge, E. U., Cutler, A. J., Pritchard, N. R., and Smith, K. G., Interleukin 4 reduces expression of inhibitory receptors on B cells and abolishes CD22 and Fc gamma RII-mediated B cell suppression. J. Exp. Med., 195, 1079-1085 (2002)   DOI   ScienceOn
7 Takayama, T., Kaneko, K., Morelli, A. E., Li, W., Tahara, H., Thomson, A. W., and Thomas, E., Retroviral delivery of transforming growth factor-beta1 to myeloid dendritic cells: inhibition of T-cell priming ability and influence on allograft survival. Transplantation, 74, 112-119 (2002)   DOI   PUBMED   ScienceOn
8 Xu, Q., Konta T., and Kitamura M., Retinoic Acid regulation of mesangial cell apoptosis. Exp. Nephrol., 10, 171-175 (2002)   DOI   PUBMED   ScienceOn
9 Yaffe, M. B., How do 14-3-3 proteins work: Gatekeeper phosphorylation and the molecular anvil hypothesis. FEBS Lett., 513, 53-57 (2002)   DOI   PUBMED   ScienceOn
10 Glynn, P. C., Henney, E., and Hall, I. P., The selective CXCR2 antagonist SB272844 blocks interleukin-8 and growth-related oncogene-alpha-mediated inhibition of spontaneous neutrophil apoptosis. Pulm. Pharmacol. Ther., 15, 103-110 (2002)   DOI   ScienceOn
11 Tylaska, L. A., Boring, L., Weng, W., Aiello, R., Charo, I. F., Rollins, B. J., and Gladue R. P., Ccr2 regulates the level of mcp-1/ccl2 in vitro and at inflammatory sites and controls T cell activation in response to alloantigen. Cytokine,18,184-190 (2002)   DOI   ScienceOn
12 Takayanagi, H., Kim, S., Matsuo, K., Suzuki, H., Suzuki, T., Sato, K., Yokochi, T., Oda, H., Nakamura, K., Ida, N., Wagner, E. F., and Taniguchi, T., RANKL maintains bone homeostasis through c-Fos-dependent induction of interferon-beta. Nature, 416, 744-749 (2002)   DOI   PUBMED   ScienceOn
13 Lindsten, T., Ross, A. J., King, A., Zong, W., Rathmell, J. C., Shiels, H. A., Ulrich, E., Waymire, K. G., Mahar, P., Frauwirth, K., Chen, Y., Wei, M., Eng, V. M., Adelman, D. M., Simon, M. C., Ma, A., Golden, J. A., Evan, G., Korsmeyer, S. J., MacGregor G. R., and Thompson, C. B., The combined functions of proapoptotic Bcl-2 family members Bak and Bax are essential for normal development of multiple tissues. Molecular Cell, 6, 1389-1399 (2000)   DOI   ScienceOn
14 Suarez, A., Mozo, L., and Gutierrez, C., Generation of CD4(+) CD45RA(+) Effector T cells by stimulation in the presence of cyclic adenosine 5'-monophosphate- elevating agents. J. Immunol., 169, 1159-1167 (2002)   PUBMED
15 Srivastava, R. and Kulshreshtha, K., Bioreactive polysaccharides from plants. Phytochem., 28, 2877-2883 (1989)   DOI   ScienceOn
16 Aittomaki, S., Yang, J., Scott, E. W., Simon, M. C., and Silvennoinen O., Distinct functions for signal transducer and activator of transcription 1 and PU.1 in transcriptional activation of Fc gamma receptor I promoter. Blood, 100, 1078-1080 (2002)   DOI   PUBMED   ScienceOn
17 Lazzarino, D. A., de Diego, M., Hirschman, S. Z., Zhang, K. Y., Shaikh, S., Musi, E., Liaw, L., and Alexander, R. J., IL-8 and MCP-1 secretion is enhanced by the peptide-nucleic acid immunomodulator, Product R, in U937 cells and primary human monocytes. Cytokine, 14, 234-239 (2001)   DOI   ScienceOn
18 Han, S. B., Park, S. H., Lee, K. H., Lee, C. W., Lee, S. H., Kim, H. C., Kim, Y. S., Lee, H. S., and Kim, H. M., Polysaccharide isolated from the radix of Platycodon grandiflorum selectively activates B cells and macrophages but not T cells. Int. Immunopharmacol., 1, 1969-1978 (2001)   DOI   ScienceOn
19 Vujaskovic, Z., Feng, Q. F., Rabbani, Z. N., Anscher, M. S., Samulski, T. V., and Brizel, D. M., Radioprotection of lungs by amifostine is associated with reduction in profibrogenic cytokine activity. Radiat. Res., 157, 656-660 (2002)   DOI   ScienceOn
20 Argiles, J. M., Busquets, S., and Lopez-Soriano, F. J., The role of uncoupling proteins in pathophysiological states. Biochem. Biophys. Res. Commun., 293, 1145-1152 (2002)   DOI   ScienceOn
21 Cusson, N., Oikemus, S., Kilpatrick, E. D., Cunningham, L., and Kelliher, M., The death domain kinase RIP protects thymocytes from tumor necrosis factor receptor type 2-induced cell death. J. Exp. Med., 196, 15-26 (2002)   DOI   ScienceOn
22 Bisson, S. A., Ujack, E. E., and Robbins, S. M., Isolation and characterization of a novel, transforming allele of the c-Cbl proto-oncogene from a murine macrophage cell line. Oncogene, 21, 3677-3687 (2002)   DOI   ScienceOn
23 Taylor, K., A modification of the phenol sulfuric acid method of total sugar determination. Appl. Biochem. Biotechnol., 53, 207-214 (1995)   DOI   ScienceOn
24 Zhu, Z., Ma, B., Zheng, T., Homer, R. J., Lee, C. G., Charo, I. F., Noble, P., and Elias, J. A., IL-13-induced chemokine responses in the lung: role of CCR2 in the pathogenesis of IL-13-induced inflammation and remodeling. J. Immunol., 168, 2953-2962 (2002)   PUBMED
25 Macian, F., Garcia-Cozar, F., Im, S. H., Horton, H. F., Byrne, M. C., and Rao, A., Transcriptional mechanisms underlying lymphocyte tolerance. Cell, 109, 719-731 (2002)   DOI   ScienceOn
26 Nakamoto, Y., Kaneko, S., and Kobayashi, K., Monocytedependent cell death of T lymphocyte subsets in chronic hepatitis C. Immunol. Lett., 78, 169-174 (2001)   DOI   ScienceOn
27 Hudson, L. and Hay, F. C., Practical Immunology. 3rd Ed. Blackwell Scientific Publications, Oxford, (1989)
28 Kim, J. R., Kwon, K. S., Yoon, H. W., Lee, S. R., and Rhee, S. G., Oxidation of proteinaceous cysteine residues by dopaminederived $H_2O_2$ in PC12 cells. Arch. Biochem. Biophys., 397, 414-423 (2002)   DOI   ScienceOn
29 Koo, K. A., Kwak, J. W., Lee, K. R., Zee, O. P., Woo, E. R., Park, H. K., and Youn, H. J., Antitumor and immunomodulating activities of the polysaccharide fractions from Artemisia selengensis and Artemisia iwayomogi. Arch. Pharm. Res., 17, 371-374 (1994)   DOI   ScienceOn
30 Kuo, K., Hsu, S., Li, Y., Lin, W., Liu, L., Chang, L., Lin, C., Lin C., and Sheu, H., Anticancer activity evaluation of the Solanum glycoalkaloid solamargine; Triggering apoptosis in human hepatoma cells. Biochem. Pharmacol., 60, 1865-1873 (2000)   DOI   ScienceOn
31 Kroemer, G., Bosca, L., Zamzami, N., Merchetti, P., Hortelano, S., and Martinez-A, C., Detection of apoptosis and apoptosisassociated alterations. In: Lefkovits I. Immunology Methods Manual, Academic Press, San Diego, pp. 1111-1125, (1997)
32 Dai, X. M., Ryan, G. R., Hapel, A. J., Dominguez, M. G., Russell, R. G., Kapp, S., Sylvestre, V., and Stanley, E. R., Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects. Blood, 99, 111-120 (2002)   DOI   ScienceOn
33 Choi, J., Conrad, C. C., Malakowsky, C. A., Talent, J. M., Yuan, C. S., and Gracy, R. W., Flavones from Scutellaria baicalensis Georgi attenuate apoptosis and protein oxidation in neuronal cell lines. Biochim. Biophys. Acta., 1571, 201-210 (2002)   DOI   PUBMED   ScienceOn
34 Strober, W., Wright-Giemsa and nonspecific esterase staining of cells., In Coligan, E. D., Kruisbeek, A. M., Margulies, D. H., Shevach, E. M. and Strober, W., (Eds) Current protocols in immunology. Wiley, New York, pp. A3.5-A3.7, (1992)