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Inhibitory Effects of Luteolin Isolated from Ixeris sonchifolia Hance on the Proliferation of HepG2 Human Hepatocellular Carcinoma Cells  

Yee, Su-Bog (Department of Pharmacy, Pusan National University)
Lee, Jung-Hwa (Department of Pharmacy, Pusan National University)
Chung, Hae-Young (Department of Pharmacy, Pusan National University)
Im, Kwang-Sik (Department of Pharmacy, Pusan National University)
Bae, Song-Ja (Department of Food and Nutrition, Silla University)
Choi, Jae-Soo (Faculty of Food Science and Biotechnology, Pukyong National University)
Kim, Nam-Deuk (Department of Pharmacy, Pusan National University)
Publication Information
Archives of Pharmacal Research / v.26, no.2, 2003 , pp. 151-156 More about this Journal
Abstract
We investigated the anti-proliferative effects of luteolin and apigenin, isolated from Ixeris sonchifolia Hance, on HepG2 human hepatocellular carcinoma cells. In MTT assay luteolin showed more efficient anti-proliferative effects on cells than apigenin did. According to propidium iodide staining and flow cytometry studies, we postulated that these effects might be a result of cell cycle arrest. Hence we examined the changes of protein expressions related to cell cycle arrest. Western blotting data demonstrated that the down-regulated expression of CDK4 was correlated to the increase of p53 and CDK inhibitor $p21^{WAF1/CIP1}$ protein. These data suggest that luteolin may have potential as an anti-cancer agent.
Keywords
Luteolin; Apigenin; Ixeris sonchifolia H.; HepG2 cells; Cell cycle arrest;
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