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Structure of Three New Terpenoids, Spiciformisins a and b, and Monocyclosqualene, Isolated from the Herbs of Ligularia fischeri var. spiciformis and Cytotoxicity  

Lee, Kyung-Tae (College of Pharmacy, Kyung-Hee University)
Koo, Sung-Ja (Department of Food and Nutrition, Kyung-Hee University)
Jung, Seung-Hee (Department of Food and Nutrition, Kyung-Hee University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Jung, Hyun-Ju (Division of Applied Plant Sciences, Sangji University)
Park, Hee-Juhn (Division of Applied Plant Sciences, Sangji University)
Publication Information
Archives of Pharmacal Research / v.25, no.6, 2002 , pp. 820-823 More about this Journal
Abstract
The diethylet er fraction from the leaves extract of Ligularia fischeri var. spiciformis (Compositae) was subjected to silica gel column chromatography and yielded three new terpenoids named spiciformisin a (1), spiciformisin b (3), and monocyclosqualene (2). Acyclic diterpenes, spiciformisin a and -b, were established as 3,7,11,15-tetramethyl-1,3(20)-hexadecadiene and 3,7,11,15-tetramethyl-1,3,6,10,14-hexadecapentaene (IUPAC), respectively. A monocyclic triterpene, monocyclosqualene, were determined as [3,8,12,16,16-pentamethyl-(3,7,11,15-hexadecatetraenyl)]-3,3,5-trimethyl-1-cyclohexene. The structures were determined on the basis of NMR and MS analysis. Spiciformicin b showed potent cytotoxicity ($IC_{50},{\;}<9.7{;\}{\mu\textrm{g}}/ml$ against HL-60) in contrast to no cytotoxicity ($IC_{50},{\;}>200{\;}}{\mu\textrm{g}}/ml against HL-60 cells) of spiciformicin a with a cis-conjugated dienyl diexomethylene.
Keywords
Ligularia fischeri var spiciformis; Compositae; Terpenoids; Spciformisin; Monocyclosqualene; Cytotoxicity;
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Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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