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Airway Mucus: Its Components and Function  

Lillehoj, Erik-P. (Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy)
Kim, K.-Chul (Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland, School of Medicine)
Publication Information
Archives of Pharmacal Research / v.25, no.6, 2002 , pp. 770-780 More about this Journal
Abstract
The airway surface liquid (ASL), often referred to as mucus, is a thin layer of fluid covering the luminal surface of the airway. The major function of mucus is to protect the lung through mucociliary clearance against foreign particles and chemicals entering the lung. The mucus is comprised of water, ions, and various kinds of macromolecules some of which possess the protective functions such as anti-microbial, anti-protease, and anti-oxidant activity. Mucus glycoproteins or mucins are mainly responsible for the viscoelastic property of mucus, which is crucial for the effective mucociliary clearance. There are at least eight mucin genes identified in the human airways, which will potentially generate various kinds of mucin molecules. At present, neither the exact structures of mucin proteins nor their regulation are understood although it seems likely that different types of mucins are involved in different functions and might also be associated with certain airway diseases. The fact that mucins are tightly associated with various macromolecules present in ASL seems to suggest that the defensive role of ASL is determined not only by these individual components but rather by a combination of these components. Collectively, mucins in ASL may be compared to aircraft carriers carrying various types of weapons in defense of airborne enemies.
Keywords
Airway surface liquid; Mucus; Defensive function; Mucus glycoprotein; Proteoglycan;
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1 Sims, D. E., Horne, M. M., Heterogeneity of the composition and thickness of tracheal mucus in rats. Am. J. Physiol., 273, L1036-L1041(1997)   PUBMED
2 Karlsson, N. G., Johansson, M. E., Asker. N., Karlsson, H., Gendler, S. J., Carlstedt, I., Hansson, G. C., Molecular characterization of the large heavily glycosylated domain glycopeptide from the rat small intestinal Muc2 mucin. Glycoconj. J., 13, 823-831 (1996)   DOI   ScienceOn
3 Kim, K. C., Possible requirement of collagen gel substratum for production of mucin-like glycoproteins by primary rabbit tracheal epithelial cells in culture. In Vitro, 21, 617-621 (1985)
4 Ligtenberg, M. J. L., Vos, H. L., Gennissen, A. M., Hilkens, J., Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini. J. Biol. Chem., 265, 5573-5578(1990)   PUBMED
5 Louahed, J., Toda, M., Jen, J., Hamid, Q., Renauld, J. C., Levitt, R. C., Nicolaides, N. C., Interleukin-9 upregulates mucus expression in the airways. Am. J. Respir. Cell Mol. Biol., 22, 649-656 (2000)   DOI   PUBMED   ScienceOn
6 Sala, A., Murphy, R. C., Voelkel, N. F., Direct airway injury results in elevated levels of sulfidopeptide leukotrienes, detectable in airway secretions. Prostaglandins, 42, 1-7 (1991)   DOI   ScienceOn
7 Thompson, A. B., Bohling, T., Payvandi, F., Rennard, S. I., Lower respiratory tract lactoferrin and lysozyme arise primarily in the airways and are elevated in association with chronic bronchitis. J. Lab. Clin. Med., 115, 148-158 (1990)   PUBMED
8 An, G., Luo, G., Wu, R, Expression of MUC2 gene is downregulated by vitamin A at the transcriptional level in vitro in tracheobronchial epithelial cells. Am. J. Respir Cell Mol. Biol., 10, 546-551 (1994)   DOI   PUBMED   ScienceOn
9 Bemacki, S. H., Nelson, A. L., Abdullah, L., Sheehan, J. K., Harris, A, William-Davis, C., Randell, S. H., Mucin gene expression during differentiation of human airway epithelia in vitro. MUC4 and MUC5b are strongly induced. Am. J. Respir Cell Mol. Biol., 20, 595-604 (1999)   DOI   PUBMED   ScienceOn
10 Holgate, S. T., Epithelial damage and response. Clin. Exp. Allergy, 30, 37-41 (2000)   DOI   ScienceOn
11 Bhaskar, K. R, O'Sullivan, D. D., Seltzer, J., Rossing, T H., Drazen, J. M., Reid, L. M., Density gradient study of bronchial mucus aspirates from healthy volunteers (smokers and nonsmokers) and from patients with tracheostomy. Exp. Lung Res., 9, 289-308 (1985)   DOI   ScienceOn
12 Carraway, K. L., Price-Schiavi, S. A, Komatsu, M., Idris, N., Perez, A, Li, P., Jepson, S., Zhu, X., Carvajal, M. E., Carraway, C. A, Multiple facets of sialomucin complexl MUC4, a membrane mucin and ErbB2 ligand, in tumors and tissues (Y2K update). Front Biosci., 5, D95-D107 (2000)   DOI   PUBMED
13 Christensen, T. G., Blanchard, G. C., Nolley, G., Hayes, J. A, Ultrastructural localization of endogenous peroxidase in the lower respiratory tract of the guinea pig. Cell Tissue Res., 214, 407-415 (1981)   PUBMED
14 Dohrman, A., Tsuda, T., Escudier, E., Cardone, M., Jany, B., Gum, J., Kim, Y., Basbaum, C., Distribution of lysozyme and mucin (MUC2 and MUC3) mRNA in human bronchus. Exp. Lurg Res., 20, 367-380 (1994)   DOI   ScienceOn
15 Gruttor, M. G., Fendrich, G., Huber, R., Bode, W., The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analyzed in its complex with bovine alpha-chymotrypsin. EMBO J., 7, 345-351 (1988)   PUBMED
16 Huang, J., Olivenstein, R., Taha, R., Hamid, Q., Ludwig, M., Enhanced proteoglycan deposition in the airway wall of atopic asthmatics. Am. J. Respir. Crit. Care Med., 160, 725-729 (1999)   DOI   PUBMED   ScienceOn
17 Meezaman, D., Charles, P., Daskal, E., Polymeropoulos, M. H., Martin, B. M., Rose, M. C., Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). J. Biol. Chem., 269, 12932-12939 (1994)   PUBMED
18 Lillehoj, E. P., Hyun, S. W., Kim, B. T., Zhang, X. G., Lee, D. I., Rowland, S., Kim, K. C., Muc1 mucins on the cell surface are adhesion sites for Pseudomonas aeruginosa. Am. J. Physiol. (Lung Cell Mol Physiol), 280, L181-L187 (2001)   DOI   PUBMED
19 Lukacs, N. W., Strieter, R. M., Chensue, S. W., Kunkel, S. L., Activation and regulation of chemokines in allergic airway inflammation. J. Leukoc. Biol., 59, 13-17 (1996)   DOI   PUBMED
20 Lundgren, J. D., Rieves, R. D., Mullol, J., Logun, C., Shelhamer, J. H., The effect of neutrophil protenase enzymes on the release of mucus from feline and human airway cultures. Respir. Med., 88, 511-518 (1994)   DOI   ScienceOn
21 Ohkavrara, Y., Tamura, G., Iwasaki, T., Tanaka, A., Kikuchi, T., Shitate, K., Activation and transforming growth factor-beta production in eosinophils by hyaluronan. Am. J. Respir. Cell Mol Biol., 23, 444-451 (2000)   DOI   PUBMED   ScienceOn
22 Park, H. R., Hyun, S. W., Kim, K. C., Expression of MUC1 mucin gene by hamster tracheal surface epithelial cells in primarv culture. Am. J . Respir. Cell Mol. Biol., 15, 237-244 (1996)   DOI   PUBMED
23 Quinn, R. J., McGuckin, M. A., Phosphorylation of the cytoplasmtc domain of the MUC1 mucin correlates with chanqes in cell-cell adhesion. Int. J. Cancer, 87, 499-506 (2000)   DOI   ScienceOn
24 Salathe, M., Holderby, M, Forteza, R, Abraham, W. M., Wanner, A., Conner, G. E., Isolation and characterization of a peroxidase from the airway. Am. J. Respir. Cell Mol. Biol., 17, 97-105 (1997)   DOI   PUBMED   ScienceOn
25 van de Bovenkamp, J. H., Hau, C. M., Strous, G. J., Buller, H. A., Dekker, J., Einerhand, A. W., Molecular cloning of human gastric mucin MUC5AC reveals conserved cysteine-rich Ddomains and a putative leucine zipper motif. Biochem. Biophys. Res. Commun., 245, 853-859 (1998)   DOI   ScienceOn
26 Singh, P. K., Jia, H. P., Wiles, K., Hesselberth, J., Liu, L., Conway, B. A., Greenberq, E. P., Valore, E. V., Welsh, M. J., Ganz, T., Tack, B. F., McCray, P. B. Jr, Production of betadefensins by human airway epithelia. Proc. Natl. Acad. Sci. USA, 95, 14961-14966 (1998)   DOI   ScienceOn
27 Smith, J., Travis, J. S. M., Greenberg, E. P., Welsh, M. J., Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface liquid. Cell, 85, 229-236 (1996)   DOI   ScienceOn
28 Thompson, R. C., Ohlsson, K., Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase. Proc. Natl. Acad. Sci. USA, 83, 6692-6696 (1986)   DOI   ScienceOn
29 van de Graaf, E. A., Out, T. A., Kobesen, A., Jansen, H. M., Lactoferrin and secretory lgA in the bronchoalveolar lavage fluid from patients with a stable asthma. Lung, 169, 275-283 (1991)   DOI   ScienceOn
30 Yoon, J. H., Kim, K. S., Kim, H. U., Linton, J. A., Lee, J. G., Effects of TNF-alpha and IL-1 beta on mucin, lysozyme, IL-6 and IL-8 in passage-2 normal human nasal epithelial cells. Acta Otolaryngol., 119, 905-910 (1999)   DOI   ScienceOn
31 Yoon, J. H., Kim, K. S., Kim, S. S., Lee, J. G., Park, I. Y.,Secretory differentiation of serially passaged normal human nasal epithelial cells by retinoic acid: expression of mucin and lysozyme. Ann. Otol. Rhinal. Laryngol., 109, 594-601 (2000)   DOI
32 Longphre, M., Li, D., Gallup, M., Drori, E., Ordonez, C. L., Redman, T., Wenzel, S., Bice, D. E., Fahy, J. V.. Basbaum, C., Allergen-induced IL-9 directly stimulates mucin transcription in respiratory epithelial cells. J. Clin. Invest., 104, 1375-1382 (1999)   DOI   PUBMED   ScienceOn
33 Zhao, J., Sime, P. J., Bringas, P. Jr, Gauldie, J., Warburton, D., Adenovirus-mediated decorin gene transfer prevents TGF-beta-induced inhibition of lung morphogenesis. Am. J. Physiol., 277, L412-L422 (1999)   DOI   PUBMED
34 Zrihan-Licht, S., Baruch, A., Elroy-Stein, O., Keydar, I., Wreschner, D. H., Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules. FEBS Lettr., 356, 130-136 (1994)   DOI   ScienceOn
35 Konstan, M. W., Walenga, R. W., Hilliard, K. A., Hilliard, J. B., Leukotriene B4 markedly elevated in the epithelial lining fluid of patients with cystic fibrosis. Am. Rev. Respir. Dis., 148, 896-901 (1993)   DOI   PUBMED
36 Zuhdi Alimam, M., Piazza, F. M., Selby, D. M., Letwin. N., Huang, L., Rose, M. C., Muc-5/5ac mucin messenger RNA and protein expression is a marker of goblet cell metaplasia in murine airways. Am. J. Respir. Cell Mol. Biol., 22, 253-260 (2000)   DOI   PUBMED   ScienceOn
37 Pigny, P, Guyonnet-Duperat, V., Hill, A. S., Pratt, W. S., Gal eque- Zouitina, S., d'Hooge, M. C., Laine, A., Van Sellnirigen, I., Degand, P., Gum, J. R., Kim, Y. S., Swallow, D. M. Aubert, J. P., Porchet, N., Human mucin qenes assigned to 11p15.5: Identification and organization of a cluster of qenes. Genomics, 38, 340-352 (1996)   DOI   ScienceOn
38 Rose, M. C., Piazza, F. M., Chen, Y. A., Ailmam, M. Z., Bautista, M. V., Letwin, N., Rajput, B., Model systems for investigating mucin gene expression in airway diseases. J. Aerosol Med., 13, 245-261 (2000)   DOI   ScienceOn
39 Chen, Y., Zhao, Y. H., Wu, R., In silico cloning of mouse Muc5b gene and upregulation of its expression in mouse asthma model. Am. J. Respir. Crit. Care Med., 164, 1059-1066 (2001)   DOI   PUBMED   ScienceOn
40 Ligtenberg, M. J. L., Kruijshaar, L., Buijs, F, van Meijer, M., Litvinov, S. V., Hilkens, J., Cell-associated episialin is a complex containing two proteins derived from a common precursor. J. Biol. Chem., 267, 6171-6177 (1992)   PUBMED
41 Nadziejkc, C. E., Siomiany, B. L., Siomiany, A., Most of the lipid in punulent sputum is bound to mucus glycoprotein. Exp. Lung Res., 19, 671-684 (1993)   DOI   ScienceOn
42 Boman, H. G., Peptide antibiotics and their role in innate immunity. Annu. Rev. Immunol., 13, 61-92 (1995)   DOI   PUBMED   ScienceOn
43 Paul, E., Lee, D. I., Hyun, S. W., Gendler, S. J., Kim, K. C., ldentiflcation and characterization of high molecular-mass mucin-like glycoproteins in the plasma membrane of airway epithel al cells. Am. J. Respir. Cell Mol. Biol., 19, 681-690 (1998)   DOI   PUBMED   ScienceOn
44 Bhaskar, K. R, O'Sullivan, D. D., Opaskar-Hincman, H., Reid, L. M., Lipids in airway secretions. Eur. J. Respir. Dis. (Suppl), 153, 215-221 (1987)
45 Buisine, M. P., Devisme, L., Copin, M. C., Durand-Reville, M., Gosselin, B., Aubert, J. P., Porchet, N., Developmental mucin gene expression in the human respiratory tract. Am. J. Respil. Cell Mol. BioI., 20, 209-218 (1999)   DOI   ScienceOn
46 Dohrman, A, Miyata, S., Gallup, M., Li, J. D., Chapelin, C., Corte, A, Escudier, E., Nadel, J., Basbaum, C., Mucin gene (MUC2 and MUC5AC) upregulation by Gram-positive and Gram-jeqative bacteria. Biochim. Biophys. Acta., 1406, 251-259 (1998)   DOI   PUBMED   ScienceOn
47 Breuer, R, Christensen, T. G., Niles, R. M., Stone, P. J., Snider, G. L., Human neutrophil elastase causes glycoconjugate release from the epithelial cell surface of hamster trachea in organ culture. Am. Rev. Respir. Dis., 139, 779-782 (1989)   DOI   PUBMED   ScienceOn
48 Perlmutter, D. H., Pierce, J. A., The alpha 1-antitrypsin gene and em physema. Am. J. Physiol., 257, L147-L162 (1989)   DOI   PUBMED
49 Baruch, A., Hartmann, M. L., Yoeli, M., Adereth, Y, Greenstein, S., Stadler, Y., Skomik, Y, Zaretsky, J., Smorodinsky, N. I., Keydar, I., Wreschner, D. H., The breast cancer-associated MUC1 qene qenerates both a receptor and its cognate binding protein. Cancer Res., 59, 1552-1561 (1999)   PUBMED
50 Moniaux, N., Escande, F., Porchet, N., Aubert, J. P., Batra, S. K., Structural organization and classification of the human mucin genes. Front. Biosci., 6, D1192-D1206 (2001)   DOI   PUBMED
51 Sharma, P., Dudus, L., Nielsen, P. A., Clausen, H., Yankaskas, J. R., Hollingsworth, M. A., Engelhardt, J. F., MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways. Am. J. Respir. Cell Mol. Biol., 19, 30-37(1998)   DOI   PUBMED   ScienceOn
52 Sallenave, J. M., Silva, A., Marsden, M. E., Ryle, A. P., Secretion of mucus proteinase inhibitor and elafin by Clara cell and type II pneumocyte cell lines. Am. J Respir. Cell Mol. Biol., 8, 126-33 (1993)   DOI   PUBMED   ScienceOn
53 Williams, S. J., Wreschner, D. H., Tran, M., Eyre, H. J., Sutherland, G. R., McGuckin, M. A., MUC13, a novel human cell surface mucin expressed by epithelial and hemopoietic cells. J. Biol. Chem., 276, 18327-18336 (2001)   DOI   ScienceOn
54 Gray, T Koo, J. S., Nettesheim, P., Regulation of mucous differentiation and mucin gene expression in the tracheobronchial epithelium. Toxicology, 160, 35-46 (2001)   DOI   PUBMED   ScienceOn
55 Hayashi, T., Takahashi, T., Motoya, S., Ishida, T, Itoh, F., Adachi, M., Hinoda, Y., Imai, K., MUC1 mucin core protein binds to the domain 1 of ICAM-1. Digestion, 63, 87-92 (2001)   DOI   PUBMED   ScienceOn
56 Kim, K. C., Mucin-like glycoproteins secreted from cultured hamster tracheal surface epithelial cells: Their hydrophobic nature and amino acid composition. Exp. Lung Res., 17, 65-76 (1991)   DOI   PUBMED   ScienceOn
57 Li, Y, Kuwahara, H., Ren, J., Wen, G., Kufe, D., The c-Src tyrosine kinase regulates signaling of the human DF3/MUC1 carcinoma-associated antigen with GSK3$\beta$3$\beta$ and $\beta$-catenin. J. Biol. Chem., 276, 6061-6064 (2001a)   DOI   ScienceOn
58 Ellison, R. T., Boose, D., LaForce, F. M., Isolation of an ant bacterial peptide from human lung lavage fluid. J. Infect. Dis., 151, 1123-1129 (1985)   DOI   PUBMED   ScienceOn
59 Li, J. D., Dohrman, A. F., Gallup, M., Miyata, S., GUill, J. R., Kim, Y. S., Nadel, J. A., Prince, A., Basbaum, C. B., Transcriptional activation of mucin by Pseudomonas aeruginosa lipopolysaccharide in the pathogenesis of cystic fibrosis lung disease. Proc. Natl. Acad. Sci. USA, 94, 967-72 (1997)   DOI   ScienceOn
60 Lopez-Ferrer, A., Curull, V., Barranco, C., Garrido, M., Lloreta, J., Real, F X., de Bolos, C., Mucins as differentiation markers in bronchial epithelium. Squamous cell carcinoma and adenocarcinoma display similar expression patterns. Am. J. Respir. Cell Mol. Biol., 24, 22-29 (2001)   DOI   PUBMED   ScienceOn
61 Pandey, P., Kharbanda, S., Kufe, D., Association of the DF3/ MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein. Cancer Res., 55, 4000-4003 (1995)   PUBMED
62 Singh, P. K., Tack B. F., McCray, P. B. Jr, Welsh, M. J.,Synergistic and additive killing by antimicrobial factors found in human airway surface liquid. Am. J Physiol. Lung Cell Mol. Physiol., 279, L799-L805 (2000)   DOI   PUBMED
63 Vogelmeier, C., Hubbard, R. C., Fells, G. A., Schnebli, H. P., Thompson, R. C., Fritz, H., Crystal, R. G., Anti-neutrophil elastase defense of the normal human respiratory epithelial surface provided by the secretory leukoprotease inhibitor. J. Clin. Invest., 87, 482-488 (1991)   DOI   PUBMED
64 Kai, H., Yoshitake, K., Hisatsune, A., Kido, T., Isohama, Y., Takahama, K., Miyata, T., Dexamethasone suppresses mucus production and MUC-2 and MUC-5AC gene expression by NCI-H292 cells. Am. J. Physiol., 271, L484-L488 (1996)   PUBMED
65 Dabbagh, K., Takeyama, K., Lee, H. M., Ueki, I. F., Lausier, J. A., Nadel, J. A., IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo. J. Immunol., 162, 6233-6237 (1999)   PUBMED
66 Gum, J. R., Hicks, J. W., Toribara, N. W., Siddiki, B., Kim, Y. S., Molecular cloning of human intestinal mucin (MUC2) cDNA. J. Biol. Chem., 269, 2440-2446 (1994)   PUBMED
67 Lillehoj, E. P., Kim, B. T., Kim, K. C., Identification of Pseudomonas aeruginosa flagellin as an adhesin for Muc1 mucin. Am. J. Physiol. (Lung Cell Mol Physiol), 282, L751-L756 (2002)   DOI   PUBMED
68 Salathe, M., Guldimann, P., Conner, G. E., Wanner, A., Hydrogen peroxide-scavenging properties of sheep airway mucus. Am. J. Respir. Crit. Care Med., 151, 1543-1550 (1995)   DOI   PUBMED   ScienceOn
69 Wu, R., Yankaskas, J., Cheng, E., Knowles, M. R., Boucher, R., Growth and differentiation of human nasal epithelial cells in culture, Serum-free, hormone-supplemented medium and proteoglycan synthesis. Am. Rev. Respir. Dis., 132, 311-320 (1985)   PUBMED
70 Kim, Y. D., Kwon, E. J., Kwon, T. K., Baek, S. H., Song, S. Y., Suh, J. S., Regulation of IL-1$\beta$-mediated MUC2 gene in NCIH292 human airway epithelial cells. Biochem. Biophys. Res. Commun., 274, 112-116 (2000)   DOI   PUBMED   ScienceOn
71 Paul, A., Picard, J., Mergey, M., Veissiere, D., Finkbeiner, W. E., Bas ba um, C. B., Glycoconjugates secreted by bovine traoheal serous cells in culture. Arch. Biochem. Biophys., 260, 75-84 (1988)   DOI   ScienceOn
72 Voynow, J. A., Selby, D. M., Rose, M. C., Mucin gene expression (MUC1, MUC2, and MUC5/5AC) in nasal epithelial cells of cystic fibrosis, allergic rhinitis, and normal individuals. Lung, 176, 345-354 (1998)   DOI   ScienceOn
73 De Watel, R, Willems, L. N., Van Muijen, G. N., Franken, C., Fransen, J. A., Dijkman, J. H., Kramps, J. A.,Ultrastructural localization of bronchial antileukoprotease in central and per pneral human airways by a gold-labeling technique using monoclonal antibodies. Am. Rev. Respir. Dis., 133, 882-890 (1986)   PUBMED
74 Kim, K. C., Singh, B. N., Association of lipids with mucins may take place prior to secretion: Studies with primary tracheal epithelial cells in culture. Biorheology, 27, 491-501 (1990b)   DOI   PUBMED
75 LaForce, F. M., Boose, D. S., Effect of zinc and phosphate on an antibacterial peptide isolated from lung lavage. Infect. Immun, 45, 692-696 (1984)   PUBMED
76 Taipale, J., Lohi, J., Saarinen, J., Kovanen, P. T., Keski-Oja, J., Human mast cell chymase and leukocyte elastase release latent transforming growth factor-beta 1 from the extracellular matrix of cultured human epithelial and endothelial cells. J. Biol. Chem., 270, 4689-4696 (1995)   DOI   ScienceOn
77 Houdret, N., Perini, J. M., Galabert, C., Scharfman, A., Humbert, P., Lamblin, G., Roussel, P., The high lipid content of respiratory mucins in cystic fibrosis is related to infection. Biochim. Biophys. Acta, 880, 54-61 (1986)   DOI   PUBMED   ScienceOn
78 Snider, G. L., Lucey, E. C., Christensen, T. G., Stone, P. J., Calore, J. D., Catanese, A., Franzblau, C., Emphysema and bronchial secretory cell metaplasia induced in hamsters by human neutrophil products. Am. Rev. Respir. Dis., 129, 155-60 (1984)   PUBMED
79 Widdicombe, J. G., Role of lipids in airway function. Eur. J. Respir. Dis., 153, 197-204 (1987)
80 Renauld, J. C., New insights into the role of cytokines in asthma. J. Clin. Pathol., 54, 577-589 (2001)   DOI   PUBMED   ScienceOn
81 Gend er, S. J., Lancaster, C. A., Taylor-Papadimitriou, J., Duhig, T., Peat, N., Burchell, J., Pemberton, L., Lalani, E. N., Wilson, D., Molecular cloning and expression of human tumorassociated polymorphic epithelial mucin. J. Biol. Chem., 265, 15286-15293 (1990)   PUBMED
82 Kim, K. C., Hincman, H. O., Bhaskar, K. R., Secretions from primary hamster tracheal epithelial cells in culture: Mucin-like glycoproteins, proteoglycans and lipids. Exp. Lung Res., 15, 299-314 (1989)   DOI   ScienceOn
83 Kirkham, S., Sheehan, J. K., Knight, D., Richardson, P. S., Thornton, D. J., Heterogeneity of airways mucus: variations in the amounts and glycoforms of the major oligomeric mucins MUC5AC and MUC5B. Biochem. J., 361, 537-546 (2002)   DOI   ScienceOn
84 Li, Y., Bharti, A., Chen, D., Gong, J., Kufe, D., Interaction of glycogen synthase kinase 3$\beta$ with the DF3/MUC1 carcinomaassociated antigen and $\beta$-catenin. Mol. Cell Biol., 18, 7216-7224 (1998)   DOI   PUBMED
85 Sommerhoff, C. P., Caughey, G. H., Finkbeiner, W. E., Lazarus, S. C., Basbaum, C. B., Nadel, J. A., Mast cell chymase. A potent secretagogue for airway gland serous cells. J. Immunol., 142, 2450-2456 (1989)   PUBMED
86 Bhaskar, K. R., O'Sullivan, D. D., Opaskar-Hincman, H., Reid, L. M., Coles, S. J., Density gradient analysis of secretions produced in vitro by human and canine airway mucosa: identification of lipids and proteoglycans in such secretions. Exp. Lung Res., 10, 401-422 (1986)   DOI   ScienceOn
87 Hilkens, J., Ligtenberg, M. J. L., Vos, H. L., Litvinov, S. V, Cell membrane-associated mucins and their adhesion-modulating property. Trends Biochem. Sci., 17, 359-363 (1992)   DOI   PUBMED   ScienceOn
88 Kim, K. C., Singh, B. N., Hydrophobicity of mucin-like glycoproteins secreted by cultured tracheal epithelial cells: Association with lipids. Exp. Lung Res., 16, 279-292 (1990a)   DOI   ScienceOn
89 Meerzaman, D., Xing, P. X., Kim, K. C., Construction and characterization of a chimeric receptor containing the cytoplasmic domain of MUC1 mucin. Am. J. Physiol. (Lung Cell Mol Physiol), 278, L625-L629 (2000)   DOI   PUBMED
90 Wang, B., Lim, D. J., Han, J., Kim, Y. S., Basbaum, C, B., Li, J. D., Novel cytoplasmic proteins of nontypeable Haemophilus influenzae up-regulate human MUC5AC mucin transcription via a positive p38 mitogen-activated protein kinase pathway and a negative phosphoinositide 3-kinase-Akt pathway. J. Biol. Ghem., 277, 949-957 (2002)
91 Kim, K. C., Hyun, S. W., Kim, B. T., Meerzaman, D., Lee, M. K., Lillehoj, E. P., Pseudomonas adhesion to MUC1 mucins: A potential role of MUC1 mucins in clearance of inhaled bacteria. In, Cilia, Mucus and Mucociliary Interactions. Salathe, M (ed). Marcel Dekker, New York, NY, pp. 217-224, (2001)
92 Noah, T. I., Blach, R., Cheng, P. W., Wood, R. E., Leigh, M. W., Nasal and bronchoalveolar lavage fluid cytokines in early cys'ic f brosis. J. Infect. Dis., 175, 638-647 (1997)   DOI   PUBMED   ScienceOn
93 Shankar, V., Gilmore, M. S., Elkins, R. C., Sachdev, G. P., A novel human airway mucin cDNA encodes a protein with unique tandem-repeat organization. Biochem. J., 300, 295-298 (1994)   DOI   PUBMED
94 Baraniuk, J. N., Shizari, T, Sabol, M., Ali, M., Underhill, C. B., Hyaluronan is exocytosed from serous, but not mucous cells, of human nasal and tracheobronchial submucosal glands. J. Investig. Med., 44, 47-52 (1996)   PUBMED
95 Guyonnet-Duperat, V., Audie, J. P., Debailleul, V., Laine, A., Buisine, M. P., Galiegue-Zouitina, S., Pigny, P., Degand, P., Aubert, J. P., Porchet, N., Characterization of the human mucin gene MUC5AC: a consensus cysteine-rich domain for 11p15 mucin qenes? Biochem. J., 305, 211-219 (1995)   DOI   PUBMED
96 Kim, K. C., Regulation of airway goblet cell mucin secretion. In, Airway Secretion: Physiological Bases for the Control of Mucus Hypersecretion. Takishima T and Shimura S (eds). Lung Biology in Health and Disease, Vol. 72, Marcel Dekker, New York, NY, pp.433-449, (1994)
97 Li, Y., Martin, L. D., Minnicozzi, M., Greenfeder, S., Fine, J., Pettersen, C. A., Chorley, B., Adler, K. B., Enhanced expression of mucin genes in a guinea pig model of allergic asthma. Am. J. Respir. Cell Mol. Biol., 25, 644-651 (2001b)   DOI   PUBMED   ScienceOn
98 Sommerhoff, C. P., Nadel, J. A., Basbaum, C. B., Caughey, G. H., Neutrophil elastase and cathepsin G stimulate secretion from cultured bovine airway gland serous cells. J. Clin. Invest., 85, 682-689 (1990)   DOI   PUBMED   ScienceOn
99 Desseyn, J. L., Aubert, J. P., Van Seuningen, I., Porchet, N., Laine, A., Genomic organization of the 3' region of the human mucin gene MUC5B. J. Biol. Chem., 272, 16873-16833 (1997)   DOI   ScienceOn
100 Nadzkejkc, C., Finkelstein, Inhibition of neutrophil elastase by mucus glycoprotein. Am. J Respir. Cell Mol. Biol., 11, 103-107 (1994)   DOI   PUBMED   ScienceOn
101 Kim, K. C., Wasano, K., Niles, R. M., Schuster, J. E., Stone, P. J., Brody, J. S., Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proc. Natl. Acad. Sci. USA, 84, 9304-08 (1987)   DOI   ScienceOn
102 Dufosse, J., Porchet, N., Audie, J. P., Guyonnet-Duperat, V., Laine, A., Van Seuningen, I., Marrakchi, S., Degand, P., Aubert, J. P., Degenerate 87-base pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to 11p15. Biochem. J., 293, 329-337 (1933)
103 Guzman, K., Gray, T. E., Yoon, J. H., Nettesheim, P., Quantitation of mucin RNA by PCR reveals induction of both MUC2 and MUC5AC mRNA levels by retinoids. Am. J. Physiol. (Lung Cell Mol Physiol), 271, L1023-L1028 (1996)   DOI
104 O'Riordan, T. G., Otero, R., Mao, Y., Lauredo, I., Abraham, W. M., Elastase contributes to antigen-induced mucociliary dysiunction in ovine airways. Am. J. Respir. Crit. Care Med., 155, 1522-1528 (1997)   DOI   PUBMED   ScienceOn
105 Wickstrom, C., Davies, J. R., Eriksen, G. V., Veerman, E. C., Carlstedt, I., MUC5B is a major gel-forming, oligomeric mucin from human salivary gland, respiratory tract and endocervix: identification of glycoforms and C-terminal cleavage. Biochem. J., 334, 685-693 (1998)   DOI   PUBMED
106 Copin, M. C., Devisme, L., Buisine, M. P, Marquette, C. H., Wurtz, A., Aubert, J. P., Gosselin, B., Porchet, N., From normal respiratory mucosa to epidermoid carcinoma: expression of human mucin genes. Int. J. Cancer, 86, 162-168 (2000)   DOI   ScienceOn
107 Gerson, C., Sabater, J., Scuri, M., Torbati, A., Coffey, R., Ab aharn, J. W., Lauredo, I., Forteza, R., Wanner, A., Sa athe, M., Abraham, W. M., Conner, G. E., The lac operoxidase system functions in bacterial clearance of ainvays. Am. J. Respir. Cell Mol. Biol., 22, 665-671 (2000)   DOI   PUBMED   ScienceOn
108 Elrod, K. C., Moore, W. R., Abraham, W. M., Tanaka, R. D., Lactoferrin, a potent tryptase inhibitor, abolishes late-phase airway responses in allergic sheep. Am. J. Respir. Crit. Care Med., 156, 375-381 (1997)   DOI   PUBMED   ScienceOn
109 Gend er, S. J., Spicer, A. P., Epithelial mucin genes. Annu. Rev.Physiol., 57, 607-634 (1995)   DOI   ScienceOn
110 Gray, T. E., Guzman, K., Davis, C. W., Abdullah, L. H., Ne tesheim, P., Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am. J. Respir. Cell Mol. Biol., 14, 104-112 (1996)   DOI   PUBMED   ScienceOn
111 Lan, M. S., Batra, S. K., Qi, W. N., Metzgar, R. S., Hollingsworth, M. A., Cloning and sequencing of a human pancreatic tumor mucin cDNA. J. Biol. Chem., 265, 15294-15299 (1990)   PUBMED
112 Nakamura, H., Yoshimura, K., McElvaney, N. G., Crystal, R. G., NeLtroohil elastase in respiratory epithelial lining fluid of individuais with cystic fibrosis induces interleukin-8 gene expression in a human bronchial epithelial cell line. J. Clin. lnvest. 89, 1478-1484 (1992)   DOI
113 Braga, V. M. M., Pemberton, L. F., Duhig, T, Gendler, S. J., Spatial and temporal expression of an epithelial mucin, Muc1, during mouse development. Development, 115, 427-437 (1992)   PUBMED
114 Roca-Ferrer, J., Mullol, J., Xaubet, A., Benitez, P., BernalSprekelsen, M., Shelhamer, J., Picado, C., Proinflammatory cytokines and eosinophil cationic protein on glandular secretion from human nasal mucosa: regulation by corticosteroids. J. Allergy Clin. Immunol., 108, 87-93 (2001)   DOI   ScienceOn
115 Forteza, R., Lieb, T., Aoki, T., Savani, R. C., Conner, G. E., Salathe, M., Hyaluronan serves a novel role in airway mucosal host defense. FASEB J., 15, 2179-2186 (2001)   DOI   ScienceOn
116 Jacquot, J., Hayem, A, Galabert, C., Functions of proteins and lipids in airway secretions. Eur. Respir. J., 5, 343-358 (1992)   PUBMED