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Potent Antimutagenic and Their Anti-Lipid Peroxidative Effect of Kaikasaponin III and Tectorigenin from the Flower of Puer-aria thunbergiana  

Park, Kun-Young (Department of Food and Nutrition, Pusan National University)
Jung, Geun-Ok (Department of Food and Nutrition, Pusan National University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Lee, Kyung-Tae (College of Pharmacy, Kyung-Hee University)
Park, Hee-Juhn (Division of Applied Plant Sciences, Sangji University)
Publication Information
Archives of Pharmacal Research / v.25, no.3, 2002 , pp. 320-324 More about this Journal
Abstract
The MeOH extract of Pueraria thunbergiana (Leguminosae) flowers and its fractions were subjected to Ames test to test the antimutagenicity. EtOAc fraction (1 mg/plate) decreased the number of revertants of Salmonella typhymurium TA100 by 95% against aflatoxin $B_1{\;}(AFB_1)$. Phytochemical isolation of the EtOAc fraction afforded four isoflavonoids (tectorigenin, glycitein, tectoridin and glycitin) and one saponin (kaikasaponin III). Though the three isoflavonoids other than tectoridin showed significant antimutagenicity, the activity of kaikasaponin III was the most potent. Kaikasaponin III (1 mg/plate) decreased the number of revertants of S. typhymurium TA 100 by 99% against $AFB_1$ but by 75% against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Tectorigenin (1 mg/plate) inhibited the $AFB_1$-induced mutagenicity by 90% and MNNG-induced one by 76%. Glycitein and glycitin were less active than tectorigenin and kaikasaponin III. This result suggested that kaikasponin III prevents the metabolic activation of $AFB_1$ and scavenge electrophilic intermediate capable of mutation. The two components with potent activities, tectorigenin and kaikasaonin III, significantly prevented the malondialdehyde formation caused by bromobenzene in the rat.
Keywords
Pueraria thunbergiana; Leguminosae; Isoflavonoid; Kaikasponin III; Antimutagenic; anti-lipid peroxidative;
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Times Cited By Web Of Science : 14  (Related Records In Web of Science)
Times Cited By SCOPUS : 17
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1 Lee, K. T., Sohn, I. C., Kim, D. H., Choi, J. W., Kwon, S. H., and Park, H. J., Hypoglycemic and hypolipidemic Effects of tectorigenin and kaikasaponin III isolated from the flowers of Pueraria thunbergiana in the streptozotocin-induced diabetic rat, and those antioxidant activity. Arch. Pharm. Res., 23, 461-466 (2000)   DOI   ScienceOn
2 Kim, H. K., Cheon, B. S., Kim, Y. H., Kim, S. Y., and Kim, H. P., Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and structure activity relationship. Biochem. Pharmacol., 58, 759-765 (1999)   DOI   PUBMED   ScienceOn
3 Lee, K. T., Sohn, I. C., Kim, Y. K., Choi, J. H., Choi, J. W., and Park, H. J., Miyamoto K. Tectorigenin, an isoflavone of Pueraria thunbergiana, induces differentiation and apoptosis on human promyelocytic leukemia Cells, Biol. Pharm. Bull., 24, 1117-1121 (2001)   DOI   ScienceOn
4 Park, H. J., Park, J. H., Moon, J. O., Lee, K. T., Jung, W T., Oh, S. R., and Lee, H. K., Isoflavone glycoside from the flower of Pueraria thunbergiana. Phytochem., 51, 147-151 (1999)   DOI   ScienceOn
5 Matsushima, T., Sugimura, T., Nagao, M., Yahagi, T., Shirai, A., and Sawamura, M., 'Short-term Tests for Detecting Carcinogens,' ed. By Norpoth KH, Gamer RC. Berlin: Springer Verlag, pp. 273-85 (1980)
6 Lee, K.T., Sohn, I. C., Park, H. J., Kim, D. W., Jung, G. O., and Park, K. Y., Essential moiety for antimutagenic and cytotoxic activity of hederagenin monodesmosides isolated from the stem bark of Kalopanax pictus. Planta Med., 66, 329-332 (2000)   DOI   ScienceOn
7 Park, H. J., Kim, D. H., Choi, J. W., Park, J. H., and Han, Y. N., A potent anti-diabetic agent from Kalopanax pictus. Arch. Pharm. Res., 21, 24-29 (1998)   DOI   PUBMED
8 Lee, K. T., Sohn, I.C., Kong, E. A., Kim, D. H., Choi, S. K., Choi, J. W., and Park, H. J., Antioxidative and cytoprotective effects of isoflavones isolated from Pueraria thunbergiana Flowers. Yakhak Hoeji, 43, 736-742 (1999)
9 You, K. M., Jong, H. G., and Kim, H. P., Inhibition of cyclooxigenase/lipoxygenase from human platelets by polyhydroxylated/methoxylated flavonoids isolated from medicinal plants. Arch. Pharm. Res., 22,18-24 (1999)   DOI   ScienceOn
10 Kim, D. H., Yu, K. W., Bae, E. A., Park, H. J., and Choi, J. W., Metabolism of kaopanaxsaponin Band H by human intestinal bacteria and antidiabetic activity of their metabolites. Biol. Pharm. Bull., 21, 360-365 (1998)   DOI   PUBMED   ScienceOn
11 Kim, Y. K., Kim, R. G., Park, S. J., Ha, J. H., Choi, J. W., Park, H. J., and Lee, K. T., Inhibitory effect of prosapogenins obtained from Kalopanax pictus on tumor necrosis factor-a and nitric oxide production in Raw 264.7 murine macrophage cells. Biol. Pharm. Bull., 25, 472-476 (2002)   DOI   ScienceOn
12 Miyao, H., Arao, T., Kinjo, J., and Nohara, T., Kaikasaponin III and soyasaponin I, major triterpene saponins of Arbus cantoniensis, act on GOT and GPT: Influence on transaminase elevation of rat liver cells concomitantly exposed to CCI$_4$ for one hour. Plant Med., 64, 5-7 (1988)   DOI   ScienceOn
13 Arao, T., Udayama, M., Kinjo, J., Nohara, T., Funakoshi, and T., Kojima, S., Preventive effects of saponins from Puerariae Radix (the root of Pueraria lobata Ohwi) on in vitro immunological injury of rat primary hepatocyte cultures. Biol. Pharm. Bull., 20, 988-991 (1997)   DOI   PUBMED   ScienceOn
14 Kim, T. J., Korean Resources Plants Vol II., Seoul National University, Seoul, pp. 232-233 (1986)
15 Maron, D. M., and Ames, B. N., Revised methods for the Salmonella mutagenicity test. Mutation Res., 113, 173-215(1983)   DOI
16 Kinjo, J., Takeshita, T., Abe, Y, Terada, N., Yamashita, H., Yamasaki, M., Takeuchi, K., Murakami, K., Tomimatsu, T., and Nohara, T., Studies on the constituents of Pueraria lobata. IV. Chemical constituents in the flowers and leaves. Chem. Pharm. Bull., 36,1174-1179 (1988)   DOI
17 Shin, K. H., Kim, Y. P., Lim, S. S., Lee, S., Ryu, N., Yamada, M., and Ohuchi, K., Inhibition of Prostaglandin E$_2$ Production by the Isoflavones Tectorigenin and Tectoridin Isolated from the Rhizome Belamcanda chinensis. Planta Med., 65, 776-777(1999)   DOI   ScienceOn
18 Ohkawa, H., Ohishi, N., and Yagi, K, Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem., 95, 351-358 (1979)   DOI   ScienceOn
19 Choi, J. W., Huh, K., Kim, S. H., Lee, K. T., Kwon, S. H., and Park, H. J., Toxicology of Kalopanax pictus extract and hematological effect of the isolated anti-rheumatoidal kalopanaxsaponin A on the Freunds complete adjuvant reagent-treated rat. Arch. Pharm. Res., 24, 119-125 (2001)   DOI   ScienceOn