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http://dx.doi.org/10.1007/s11816-010-0152-1

Role of ${\alpha}$-tocopherol in cellular signaling: ${\alpha}$-tocopherol inhibits stress-induced mitogen-activated protein kinase activation  

Hyun, Tae-Kyung (Department for Plant Physiology, Institute for Plant Science, Karl-Franzens University Graz)
Kumar, Kundan (National Institute of Plant Genome Research)
Rao, Kudupudi Prabhakara (National Institute of Plant Genome Research)
Sinha, Alok Krishna (National Institute of Plant Genome Research)
Roitsch, Thomas (Department for Plant Physiology, Institute for Plant Science, Karl-Franzens University Graz)
Publication Information
Plant Biotechnology Reports / v.5, no.1, 2011 , pp. 19-25 More about this Journal
Abstract
Tocopherols belong to the plant-derived poly phenolic compounds known for antioxidant functions in plants and animals. Activation of mitogen-activated protein kinases (MAPK) is a common reaction of plant cells in defense-related signal transduction pathways. We report a novel non-antioxidant function of ${\alpha}$-tocopherol in higher plants linking the physiological role of tocopherol with stress signalling pathways. Pre-incubation of a low concentration of $50{\mu}M$ ${\alpha}$-tocopherol negatively interferes with MAPK activation in elicitor-treated tobacco BY2 suspension culture cells and wounded tobacco leaves, whereas pre-incubated BY2 cells with ${\alpha}$-tocopherol phosphate did not show the inhibitory effect on stimuli-induced MAPK activation. The decreased MAPK activity was neither due to a direct inhibitory effect of ${\alpha}$-tocopherol nor due to the induction of an inhibitory or inactivating activity directly affecting MAPK activity. The data support that the target of ${\alpha}$-tocopherol negatively regulates an upstream component of the signaling pathways that leads to stress dependent MAPK activation.
Keywords
${\alpha}$-Tocopherol; MAPKs; Non-antioxidan Stress; E-Fol;
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