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http://dx.doi.org/10.1007/s11816-010-0134-3

Sll0396 regulates transcription of the phycocyanin genes in Synechocystis sp. PCC 6803  

Oh, In-Hye (Department of Biology and Medicinal Science, PaiChai University)
Kim, Ho-San (Mass Spectrometer Research Center, Korea Basic Science Institute)
Chung, Young-Ho (Mass Spectrometer Research Center, Korea Basic Science Institute)
Kim, Young-Hye (Mass Spectrometer Research Center, Korea Basic Science Institute)
Park, Young-Mok (Mass Spectrometer Research Center, Korea Basic Science Institute)
Publication Information
Plant Biotechnology Reports / v.4, no.3, 2010 , pp. 193-199 More about this Journal
Abstract
An olive-green mutant was generated in Synechocystis sp. strain PCC 6803 by inactivation of the sll0396 gene. Whole-cell absorption spectra of the mutant revealed the missing of phycocyanin peak. An investigation of the low-temperature fluorescence emission spectra revealed that the $sll0396{\Omega}$ mutant has a reduced amount of phycocyanin. Western blot analysis showed that the mutant contained less phycocyanin ${\beta}$- and ${\alpha}$-subunits and lacked the 30- and 32-kDa linker polypeptides, and northern blot analysis revealed that the transcription of the 1.4-kb cpcBA gene encoding the phycocyanin ${\beta}$- and ${\alpha}$-subunits was lower in the mutant. The Sll0396 protein has a DNA-binding motif and shares homology with known response regulators. Our results indicate that Sll0396 plays a regulatory role in the transcription of the phycocyanin genes during phycobilisome synthesis.
Keywords
Interposon mutagenesis; Phycobilisomes; Phycocyanin; Response regulator; Synechocystis sp. PCC 6803; Transcription regulator;
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