Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100
![]() |
HwangBo, Kwon
(Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Son, Su-Hyun (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Lee, Jong-Suk (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Min, Sung-Ran (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Ko, Suk-Min (Eugentech Inc., KRIBB) Liu, Jang-R. (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Choi, Dong-Su (Department of Biology, Kunsan National University) Jeong, Won-Joong (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) |
1 | Ward AC (1992) Rapid analysis of yeast transformants using colony- PCR. Biotechniques 13:350 |
2 | Tebbe CC, Vahjen W (1993) Interference of humic acids and DNA extracted from soil in detection and transformation of recombinant DNA from bacteria and yeast. Appl Environ Microbiol 59:2657-2665 |
3 | Tsai YL, Olson BH (1992) Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Appl Environ Microbiol 58:2292-2295 |
4 | Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506-513 |
5 | Cao M, Fu Y, Guo Y, Pan J (2009) Chlamydomonas (Chlorophyceae) colony PCR. Protoplasma 235:107-110 DOI |
6 | Furukawa K, Bahavanandan VP (1983) Influences of anionic polysaccharides on DNA synthesis in isolated nuclei by DNA polymerase alpha: correlation of observed effects with properties of polysaccharides. Biochim Biophys Acta 740:466-475 DOI ScienceOn |
7 | Holmes AR, Cannon MG, Shepherd MG (1994) Detection of Candida albicans and other yeast in blood by PCR. J Clin Microbiol 32:228-231 |
8 | Berthold DA, Best BA, Malkin R (1993) A rapid DNA preparation for PCR from Chlamydomonas reinhardtii and Arabidopsis thaliana. Plant Mol Biol Rep 11:338-344 DOI ScienceOn |
9 | Panaccio M (1991) PCR based diagnosis in the presence of 8% (v/v) blood. Nucleic Acid Res 19:291-292 |
![]() |