Browse > Article

Plant Regeneration through Micropropagation from Nodal Explants of Critically Endangered and Endemic Plant Exacum travancoricum Bedd  

Elangomathavan R. (Central Institute of Medicinal and Aromatic Plants (CIMAP))
Prakash S. (Entomology Research Institute, Loyola College)
Kathiravan K. (Department of Biotechnology, University of Madras)
Seshadri S. (Shri AMM Murugappa Chettiar Research Centre (MCRC))
Ignacimuthu S. (Entomology Research Institute, Loyola College)
Publication Information
Journal of Plant Biotechnology / v.8, no.1, 2006 , pp. 51-55 More about this Journal
Abstract
A rapid micropropagation protocol was established for Exacum travancoricum Bedd. The effect of two cytokinins viz. BA and kinetin were studied to evaluate the propagation of plants through nodal explants. MS medium supplemented with 13.32 ${\mu}M$ BA induced early bud break and subsequent production of multiple shoots. Rooting of shoots occurred when cultured on 1/2 strength MS medium supplemented with 14.7 ${\mu}M$ IBA. Rooted plants were acclimatized to greenhouse conditions. The propagated plants were transferred successfully to field with 65% success. As the plant was amenable to propagation in vitro, this can be employed as a tool for conservation of this critically endangered and endemic ornamental herb.
Keywords
Endangered plant species; Exacum travancoricum; in vitro propagation; multiple shoots; nodal explants;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kozlina BP, Vranjes VK, Slade D (1997) In vitro propagation of Fibigia triquetra (DC.) Biess, a rare stenoendemic species. Plant Cell Tiss Org Cult. 51: 141-143   DOI
2 Davis C (1995) Centres of Plant Diversity-A guide and strategy for their conservation Vol.2. Asia, Australia and the Pacific, WWF,IUCN
3 Gopalan R, Henry AN (2000) Endemic plants of India Camp for the strict endemics of Agasthiamalai Hills, Southern Western Ghats, Bishen Singh Mahendrapal Singh Publication, Dehra Dun, India. pp 476
4 Henry AN, Kumari GR, Chithra V (1987) Flora of Tamil Nadu, India series I: Analysis Vol 2, Botanical Survey of India, Coimbatore
5 Santos MC, Esquibel MA, Santos Dos AVP (1990) In vitro propagation of the alkaloid producing plant Datura insignis Barb. Rodr. Plant Cell Tiss Org Cult 21: 75-78   DOI
6 Henry AN, Chandrabose M, Swaminathan MS, Nair NC (1984) Agasthiamalai and its environs: A potential area for a Biosphere Reserve. J Bombay Natural History Soc 81: 282-290
7 Prakash S, Kathiravan K, Seshadri S, Janarthanan S, Ignacimuthu S (2001) In vitro propagation and genetic stability of Hybanthus enneaspermus plants. J Trop Med Plants 2: 253-258
8 Ahuja A, Verma M, Grewal S (1982) Clonal propagation of Ocimum species through tissue culture. Indian J Exp Biol 20: 455-458
9 Sauer A Walther F, Preil W (1985) Different suitability for in vitro propagation of rose cultivars. Gartenbaumnissenschaft. 50: 133-38
10 Brandt K (1992) Micropropagation of Campanula isophylla M. Plant Cell Tiss Org Cult 29: 31-33   DOI
11 Gamble JS, Fischer CEC (1956) Flora of Presidency of Madras Vol 3. Oxford Publication, New Delhi
12 Fracaro F, Echeverrigaray S (2001) Micropropagation of Cunila galioides, a popular medicinal plant of south Brazil. Plant Cell Tiss Org Cult 64:1-4   DOI   ScienceOn
13 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497   DOI
14 Pattnaik SK, Chand PK (1996) In vitro propagation of the medicinal herbs. Ocimum americanum L. Syn, O. canum Sims. (Hoary basil) and O. sanctum L. (Holy basil). Plant Cell Rep 15: 846-850   DOI   ScienceOn
15 Sudha GC, Seeni S (1994) In vitro propagation and field establishment of Adhatoda beddomei C B Clarke a rare medicinal plant. Plant Cell Rep 13: 203-207
16 Korach AR, Juliani JR, Juliani HR, Trippi VS (1997) Micropropagation and acclimatization of Hedeoma multifloram. Plant Cell Tiss Org Cult 48: 213-217   DOI
17 Elangomathavan R, Prakash S, Kathiravan K, Seshadri S, Ignacimuthu S (2003) High frequency in vitro propagation of Kidney Tea Plant. Plant Cell Tiss Org Cult 72: 8386