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Authentication of Korean Panax ginseng from Chinease Panax ginseng and Panax quinquefolius by AFLP analysis  

Kim Bo-Bae (Department of Biology, Kongju National University)
Jeong Jae-Hun (Department of the Development of Medicinal Resources and Horticulture, Namdo Provincial College of Jeonnam)
Jung Su-Jin (Colleges of Forest Sciences, Kangwon National University)
Yun Doh-Won (RDA, National Institute of Agricltural Biotechnology)
Yoon Eui-Soo (Department of Biology, Kongju National University)
Choi Yong-Eui (Colleges of Forest Sciences, Kangwon National University)
Publication Information
Journal of Plant Biotechnology / v.7, no.2, 2005 , pp. 81-86 More about this Journal
Abstract
Panax ginseng is one of the most important medicinal plants in the world. The international trade of ginseng is increasing yearly. The disguise of Chinese and American ginseng into Korean ginseng became a problem in recent years in abroad and Korea. An effective method to authenticate the Korean Panax ginseng from others at a DNA level is necessary for the healthy development of the ginseng market. Amplified fragment length polymorphism (AFLP) analysis was applied to develop a method for the identification of Korean ginseng between Chinese ginseng and American ginseng. It is very difficult to detect the different polymorphic bands among Korean field cultivated ginseng, and between field and wild-cultivated ginseng. The genetic distance coefficient by AFLP analysis between field- and wild cultivated Korean ginseng was very low, 0.056. Whereas, polymorphic bands between Korean and Chinese wild-cultivated ginseng was significantly different. The genetic distance coefficient between wild-cultivated Korean and Chinese ginseng was 0.149. The genetic distance coefficients between the P. ginseng and P. quinquefolius were ranging from 0.626 to 0.666. These results support that the AFLP analysis could be applied to authenticate Korean P. ginseng from others Chinese P. ginseng and American ginseng (P. quinquefolius).
Keywords
AFLP; authentication; PCR; Panax ginseng; Panax quinquefolius;
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