[KSCI] Korea Science Citation Index Service

Regeneration and Agrobacterium - Mediated Transient Transformation of Button Daisy (Leucanthemum vulgare)

Franklin G. (Crop Improvement Division, Sugarcane Breeding Institute)
Alaiwi W. Abou (Crop Improvement Division, Sugarcane Breeding Institute)
Goldman S.L. (Crop Improvement Division, Sugarcane Breeding Institute)

Publication Information

Journal of Plant Biotechnology / v.7, no.1, 2005 , pp. 37-43 More about this Journal

Abstract

Explants of button daisy were screened for their regeneration potential and transient GUS gene expression. Medium containing MS salts minerals and $B_5$ vitamins supplemented with $0.1\;\cal{mg/L}$ BA and $0.1\;\cal{mg/L}$ TDZ showed the best regeneration. Disc florets and receptacles were the most responsive explants in regeneration and transient gene expression respectively. Regenerated plants were successfully rooted and established in the green-house conditions. Infection and co-cultivation of explants with Agrobacterium tumefaciens containing pCAMBIA 1301 resulted in transient GUS foci. Among the different explants, receptacles showed the highest percentage of transient GUS gene expression. Enzymatic and molecular analyses of transformed calli confirmed the integration of GUS gene.

Keywords

disc floret; receptacle; thidiazuron; GUS gene; T-DNA transfer;

Citations & Related Records

Reference

1	Boase MR, Bradley JM, Borst NK (1998) Genetic transformation mediated by Agrobacterium tumefaciens of florists chrysanthemum (Dendranthema x grandiflora) cultivar 'Peach Margaret'. In vitro Cell Dev Biol- Plant 34:46-51 DOI
2	Dwivedi AK, Banerji BK, Chackrabarty D, Mandai AKA, Datta SK (2000) Gamma ray induced new flower colour chimera and its management through tissue culture. Ind J Agric Sci 70:853-855
3	George J, Rajasekaran T, Ravishankar GA (1999) A modified culture vessel for improved callus growth and pyrethrins content of pyrethrum (Chrysanthemum cinerariaefolium Vis.). Pyrethrum Post 20:49-54
4	Hitmi A, Barthomeuf C, Sallanon H (1999) Rapid mass propagation of Chrysanthemum cinerariaefolium vis by callus culture and ability to synthesize pyrethrins. Plant Cell Rep 19: 156-160 DOI ScienceOn
5	Howarth S, Williams J (1968) Biological flora of the British Isles. J Ecology 56: 585-595 DOI ScienceOn
6	Magioli C, Rocha APM, de Oliveira DE, Mansur E (1998) Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Rep 17:661-663 DOI ScienceOn
7	Olhoft PM, Lin K, Galbraith J, Nielsen NC (2001) The role of thiol compounds in increasing Agrobacterium- mediated transformation of soybean cotyledonary-node cells. Plant Cell Rep 20: 731-737 DOI ScienceOn
8	Sagarieshvili TG (2002) Essential oils of Lettcanthemum vulgare. Chem Nat Compounds. 38: 295-296 DOI ScienceOn
9	Franklin G, Carpenter L, Reddy SC, AI-Abed D, Abou Alaiwi W, Parani M, Sairam RV (2004) Factors affecting regeneration of soybean from mature and immature cotyledons. Plant Growth Regul 43: 73-79 DOI ScienceOn
10	Franklin G, Lakshmi Sita G (2003) Agrobacterium tumefaciens mediated transformation of eggplant (Solanum melongena L.) using root explants. Plant Cell Rep 21 :549-554 DOI
11	Murch SJ, Choffe KL, Victor JMR, Slimmon TY, Krishnaraj S, Saxena PK (2000) Thidiazuron-induced plant regeneration from hypocotyls cultures of St. John's wort (Hypericum perforatum. Cv 'Anthos'). Plant Cell Rep 19:576-581 DOI ScienceOn
12	Teixeira da Silva JA (2003) Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnol Adv 21: 715-766 DOI PUBMED ScienceOn
13	Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Plainview, New York
14	Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: O-glucronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907
15	Chilton MD, Currier TC, Ferrand SK, Bendich AJ, Gordon MP, Nester EW (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci USA 71: 3672-3676 DOI
16	Chackrabarty D, Mandai AKA, Datta SK (2000) SEM and light microscopic studies on shoot regeneration from ray florets of Chrysanthemum. Israel J Plant Sci 48: 105-107 DOI ScienceOn
17	Grieve M (1995) Botanical.com: A Modern Herbal. Accessed on Aug 31st 2004
18	Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 DOI
19	Hansen G (2000) Evidence of Agrobacterium-induced apoptosis in maize cells. Mol Plant Microbe Interact 13:649-657 DOI ScienceOn
20	Ledger SE, Deroles SC, Given NK (1991) Regeneration and Agrobacterium-mediated transformation of chrysanthemum. Plant Cell Rep 10: 195-199 DOI ScienceOn
21	Roberts JC (1976) Born in the spring. Athens, OH: Ohio University Press. 159 p
22	Khalafalla MM, Hattori K (1999) A combination of thidiazuron and benzyladenine promotes multiple shoot production from cotyledonary node explants of faba bean (Vicia faba L.) Plant Growth Regul 27: 145-148 DOI ScienceOn
23	Tanaka K, Kanno Y, Kudo S, Suzuki M (2000) Somatic embryogenesis and plant regeneration in chrysanthemum (Oendranthema grandiflorum (Ramat.) Kitamura). Plant Cell Rep 19:946-953 DOI ScienceOn
24	Tzfira T, Jensen CS, Wang W, Zuker A, Vinocur B, Altman A, Vainstein A (1997) Transgenic Populus tremula: a stepby-step protocol for its Agrobacterium-mediated transformation. Plant Mol Biol Rep 15: 219-235 DOI ScienceOn
25	Van Wordragen MF, de Jong J, Schomagel MJ, Dons HJM (1992) Rapid screening for host-bacterium interactions in Agrobacterium-mediated gene transfer to chrysanthemum, by using the GUS-intron gene. Plant Sci 81 :207-214 DOI ScienceOn
26	Fukai S, de Jong J, Rademaker W (1995) Efficient gentic transformation of chrysanthemum (Dendranthema x grandiflorum (Ramat.) Kitamura) using stem segments. Breed Sci 45: 179-184
27	Mizutani T, Tanaka T (1994) Study on the floret culture of Higo- chrysanthemum. Proc School Agric, Kyushu Tokai Univ 13:9-14