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Characterizing Salt Stress Response in a Rice Variety and Its Salt Tolerant Lines Derived from In Vitro Mutagenesis  

Lee In Sok (Korea Atomic Energy Research Institute)
Kim Dong Sub (Korea Atomic Energy Research Institute)
Kang Si Yong (Korea Atomic Energy Research Institute)
Wi Seung Gon (Korea Atomic Energy Research Institute)
Jin Hua (Department of Horticulture, Chungnam National Univ., Institute of Biological Resources and Environmental Research, Da Lian Nationalties University)
Yun PiI-Yong (Department of Horticulture, Chungnam National Univ.)
Lim Yong Pyo (Department of Horticulture, Chungnam National Univ.)
Lee Young Il (Korea Atomic Energy Research Institute)
Publication Information
Journal of Plant Biotechnology / v.6, no.4, 2004 , pp. 205-212 More about this Journal
Abstract
The objectives were to compare the salt tolerance levels in the parental rice cultivar, Dongjinbyeo, and induced mutagenesis derived its lines for plant height, MDA, ATPase, POD, and 2-dimensional protein electrophoresis pattern in NaCl-containing hydroponic nutrient solutions. Rice plants isolated from a population of rice (Oryza sativa L. cv. Dongjinbyeo) mutation lines, which were generated in combination with in vitro selection and gamma-ray, exhibited salt tolerance. Line No. 18 had the longest plant, whereas NaCl-sensitive line (No. 25) had the shortest plant. The parent, and the sensitive line showed severe damage from salt stress. Tolerant lines (No. 18, 50) had a lower malonaldehyde (MDA) content than the sensitive one (Dongjinbyeo, No. 25) during salt stress. Several proteins showed significant quantitative variation through 2DE; phosphoribulokinase, peroxidase, oxygen evolving enhancer 1 and the $H^+-ATPase$, which are known to be involved in salt tolerance. The effect of salt on peroxidase and $H^+-ATPase$ activity in the seedlings of two groups with contrasting genotypes of rice was studied. A greater activity was recorded in the tolerant lines as compared to the sensitive ones (P<0.05, Duncan's test). The results indicate that salt tolerant lines expressed more salt stress-inducible proteins associated with salt tolerance than the sensitive lines during salt stress.
Keywords
ATPase; 2-dimensional electrophoresis; MDA; mutation; POD; salt-tolerance; Summary;
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