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Somatic Embryogenesis in a Range of Genotypes and Genetic Stability of the Plants Derived from Somatic Embryos Using Morphological and RAPD Markers in Sweet Potato  

Sharma, Sonali Dixit (Avestha Gengraine Technologies Pvt. Ltd.,)
Ghosh, Sangeeta Ahuja (Department of Biochemistry and Molecular Genetics, University of Virginia)
Mandal, Binay Bhushan (Tissue Culture and Cryopreservation Unit, NBPGR)
Srivastava, Prem Shanker (Centre for Biotechnology, Jamia Hamdard)
Publication Information
Journal of Plant Biotechnology / v.6, no.2, 2004 , pp. 119-124 More about this Journal
Abstract
For long-term conservation of germ plasm, somatic embryos of sweet potato are important because shoot tips are not amenable to liquid nitrogen storage. Somatic embryos from different genotypes were used for induction of somatic embryogenesis in a large number of genotypes. Somatic embryogenesis was induced on 2,4-D medium in all the 11 genotypes, collected from geographically distinct locations. Genetic fidelity of the regenerated plants was confirmed by morphological and RAPD markers.
Keywords
RAPD markers; somatic embryogenesis; sweet potato; genetic stability;
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