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Effective Acclimation System for in Vitro Regenerated Plant lets of Soybean  

Kim, Young Jin (Honam Agricultural Research Institute, National Institute of Crop Science)
Park, Tae Il (Honam Agricultural Research Institute, National Institute of Crop Science)
Kim, Hyun Soon (Honam Agricultural Research Institute, National Institute of Crop Science)
Suh, Sug Kee (Honam Agricultural Research Institute, National Institute of Crop Science)
Kim, Hag Sin (Honam Agricultural Research Institute, National Institute of Crop Science)
Yun, Song Joong (Honam Agricultural Research Institute, National Institute of Crop Science)
Publication Information
Journal of Plant Biotechnology / v.6, no.2, 2004 , pp. 107-111 More about this Journal
Abstract
To establish an efficient acclimation system for regenerated plantlets of soybean, we used various media with hydroponic nutrient solutions before regenerants were transplanted into soil. The hydroponic nutrient solution was essential for the survival of the plantlets. The vermiculite with nutrient solution at pH 5.5 was found to be the best medium with 97-100% survival rate and better growth of regenerants plantlets. Regeneraed grew best in the following order of solutions: Yoshida solution, modified Yoshida solution, SoyI, Soy II, and MS medium. However, Soy I solution (EC 2.9 mS/cm), developed by the Honam Agricultural Research Institute proved to be the most effective for acclimation in terms of the time required for vigorous growth and economical use of chemicals.
Keywords
Acclimation; regenerated plantlet; vermiculite; hydroponic system; soybean;
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  • Reference
1 Barwale UB Kerns HR, Widholm JM (1986) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167: 473-481
2 Ranch JP, Oglesby L, Zielinski AC (1986) Plant regeneration from tissue cultures of soybean by somatic embryogenesis. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants pp 97-110 Academic Press, New York
3 Wright MS, Launis KL, Novitzky R, Duesing JH, Harms CT (1991) A simple method for the recovery of multiple fertile plants from individual somatic embryos of soybean [Glycine max (L) Merrill]. In Vitro Cell Dev Biol 27: 153-157
4 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-479
5 Liu W, Moore PJ, Collins GB (1992) Somatic embryogenesis in soybean via somatic embryo cycling. In Vitro Cell Dev Biol28: 53-160
6 Kim J, LaMotte CE, Hack E (1990) Plant regeneration In Vitro from primary leaf nodes of soybean (Glycine max) seedlings. J Plant Physiol 136: 664-669
7 Dan Y, Reichert NA (1998) Organogenic regeneration of soybean from hypocotyl explants. In Vitro Cell Dev Biol-Plant 34: 14-21
8 Terzi M, Loschiavo F. (1990) Somatic embryogenesis. In: Bhojwani SS (ed) Development in crop science 19: Plant, pp 54-66 tissue culture: applications and limitations. Elsevier, Tokyo
9 Yeh M-S, Chyuan J-H (1991) In vitro culture of immature soybean embryos III. Organogenesis studies on soybean embryogenic axes culture. J Agric Assoc China 40: 77-90