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Transformation of Citrus with Coleopteran Specific $\delta$-Endotoxin Gene from Bacillus thuringiensis ssp. tenebrionis  

Rhim, Seong Lyul (Department of Genetic Engineering, Hallym University)
Kim, Il Gi (Department of Genetic Engineering, Hallym University)
Jin, Tae Eun (Department of Genetic Engineering, Hallym University)
Lee, Jin Hyoung (Department of Genetic Engineering, Hallym University)
Kuo, Ching I (Institute of Botany, Academia Sinica)
Suh, Suk Chul (National Institute of Agricultural Biotechnology)
Huang, Li Chun (Institute of Botany, Academia Sinica)
Publication Information
Journal of Plant Biotechnology / v.6, no.1, 2004 , pp. 21-24 More about this Journal
Abstract
A modified $\delta$-endotoxin gene of Bacillus thuringiensis ssp. tenebrionis (B.t.t.), encoding a coleoptera-specific toxin, was utilized to transform citrus plants, Citrus reticulata Blanco 'Ponkan' mandarian. By co-culturing the nucelli with Agrobacterium tumefaciens harboring the modified gene in the binary vector pBinAR-Btt, the chimeric toxin gene was transferred into citrus plants. The transgenic plants were selected on modified Murashige and Skoog medium containing kanamycin. Hybridization experiments demonstrated that the transgenic plants contained and expressed the toxin protein gene.
Keywords
Bacillus thuningiensis; $\delta$ -endotoxin gene; transgenic citrus plants;
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