[KSCI] Korea Science Citation Index Service

A Unique Strategy for Recovering Recombinant Proteins from Molecular Farming: Affinity Couture on Engineered Oilbodies

Seon, Jeong-Hoon (SemBioSys Genetics Inc., Canada)
J.Steven Szarka (SemBioSys Genetics Inc., Canada)
Maurice M. Moloney (SemBioSys Genetics Inc., Canada)

Publication Information

Journal of Plant Biotechnology / v.4, no.3, 2002 , pp. 95-101 More about this Journal

Abstract

Molecular faming has the potential to provide large amounts of recombinant protein for use in diagnostics and as therapeutics. Various strategies have been developed to enhance the expression level, stability, and native folding of recombinant proteins produced in plants. Few investigations into the subcellular distribution of recombinant proteins within plant cells have been published despite the potential to increase the expression level and impact the purification process. This review article discusses the current strategies used for targeting recombinant proteins to various subcellular locations and the advantages of targeting to seed oil bodies for molecular farming applications. Specifically, the affinity capture of antibodies using recombinant oilbodies is discussed.

Keywords

Endoplasmic reticulum; molecular farming; oilbody; oleosin; recombinant protein; subcellular localization;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

Reference
Cited By KSCI

1	Upstream sequences regulating legumin gene expression in heterologous transgenic plants / [ Baumlein H;Boerjan W;Nagy I;Panitz R;Inze D;Wobus U ] / Mol Gen Geneti
2	Production and purification of recombinant hirudin from plant seeds / [ Parmenter DL;Boothe JG;Moloney MM;MRL Owen(ed.);J Pen(ed.) ] / Transgenic plants: A production system for industrial and pharmaceutical proteins
3	Foreign protein production in plant tissue cultures / [ Doran PM ] / Curr Opin Biotechnol DOI ScienceOn
4	A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice / [ Chargelegue D;Vine ND;van Dolleweerd CJ;Drake PMW;Ma JK-C ] / Transgenic Research DOI ScienceOn
5	Production of antibodies in transgenic plants / [ Hiatt A;Cafferkey R;Bowdish K ] / Nature DOI ScienceOn
6	Subcellular targeting and purification of recombinant proteins in plant production systems / [ Moloney MM;Holbrook La ] / Biotechnol Genet Eng Rev DOI ScienceOn
7	Seed as repositories of recombinant proteins in molecular farming / [ Moloney MM ] / Korean Journal of Plant Tissue Culture 과학기술학회마을
8	Production of biologically active hirudin in plant seeds using oleosin partitioning / [ Parmenter DL;Booth JG;van Rooijen GJH;Yeung EC;Moloney MMM ] / Plant Mol Biol DOI ScienceOn
9	Affinity-purification of a TMV-specific recombinant full size antibody from a transgenic tobacco suspension culture / [ Fischer R;Liao YC;Drossard J ] / J Immunol Meth DOI
10	The plantibody approach: Expression of antibody genes in plants to modulate plant metabolism or to obtain pathogen resistance / [ De Jaeger G;Dewilde C;Eeckhout D;Fiers E;Depicker A ] / Plant Mol Biol DOI ScienceOn
11	GFP imaging: methodology and application to investigate cellular compartmentation in plants / [ Hanson MR;Kohler RH ] / J Exp Bot DOI
12	The C-terminal KDEL sequence increases the expression level of a single chain anti body designed to be targeted to both the cytosol and secretory pathway in transgenic tobacco / [ Schouten ARJ;van Engelen FA;de Jong GAM;Borst-Vrenssen AWM;Zilverentant JF;Bosch D;Stiekema WJ;Gommer FJ;Schots A;Bakker J ] / Plant Mol Biol DOI ScienceOn
13	Secretion of a functional single chain Fv protein in transgenic tobacco plants and cell suspension cultures / [ Firek S;Draper J;Owen MRL;Gandecha A;Cockburn B;Whitelam GC ] / Plant Mol Biol DOI ScienceOn
14	Transgenic plants for therapeutic proteins:linking upstream and downstream strategies / [ Cramer CL;Boothe JG;Oishi KK;Hammond J(ed.);McGarvey P(ed.);Yusibov V(ed.) ] / Current topics in microbiology and immunology vol 240 plant biotechnology: New products and applications
15	Plant seed oil bodies as carriers for foreign proteins / [ Van Rooijen GJH;Moloney MM ] / Bio/Technology DOI
16	Motif trap: A rapid method to clone motifs that can target proteins to defined subcellular localizations / [ Bejarano LA;Gonzalez C ] / Journal of Cell Sci
17	Recombinant proteins via oleosin partitioning / [ Moloney MM;van Rooijen GJH ] / Inform
18	Isolation of a mutant Arabidopsis plant that lacks N-acetyl glucosaminyl transferase Ⅰand is unable to synthesize golgi-modified complex N-linked glycan / [ Von Schaewen A;Sturm A;O'neill J;Chrispeels MJ ] / Plant Physiol DOI
19	Structure, biosynthesis, and function of plant glycoprotein glycans / [ Faye L ] / Current Topics in Plant Biochem. And Physiol
20	A novel seed protein gene from Vicia faba is developmentally regulated in transgenic tobacco and Arabidopsis plants / [ Baumlein H;Boerjan W;Nagy I;Bassuner R;van Montagu M;Inze D;Wobus U ] / Mol Gen Genet DOI
21	Synthesis of a functional anti-phytochrome single chain Fv protein in transgenic tobacco / [ Owen MRL;Gandecha A;Cockburn B;Whitelam G ] / Bio/technology DOI ScienceOn
22	Affinity capture of proteins from solution and their dissociation by contact printing / [ Bernard A;Fitzli D;Sonderegger P;Delamarche E;Michel B;Boshard HB;Biebuyck H ] / Nature Biotech DOI ScienceOn
23	Compartment-specific accumulation of recombinant immunoglobulins in plant cells: An essential tool for antibody production and immunomodulation of physiological functions and pathogen activity / [ Conrad U;Fiedler U ] / Plant Mol Biol DOI
24	Optimization of scFv antibody production in transgenic plants / [ Fiedler U;Phillips J;Artsaenko O;Conrad U ] / Immunotechnology DOI ScienceOn
25	Towards molecular farming in the future: Moving from diagnostic protein and antibody production in microbes to plants / [ Fisher R;Drossard J;Cmmandeur U;Schillberg S;Emans N ] / Biotechnol Appl Biochem
26	Improving scFv antibody expression levels in plant cytosol / [ Schouten ARJ;de Jong BJM;Wilmink A;Rosso MN;Bosch D;Stiekema WJ;Gommers FJ;Bakker J;Schots A ] / FEBS Lett DOI ScienceOn
27	Plant seed oil bodies as an immobilization matrix for a recombinant xylanase from the lumen fungus Neocallimastix patriciarum / [ Liu JH;Selinger LB;cheng KJ;Beauchemin KA;Moloney MM ] / Molecular Breeding DOI ScienceOn