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Parentage Identification of 'Daebong' Grape (Vitis spp.) Using RAPD Analysis  

Kim, Seung-Heui (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Jeong, Jae-Hun (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Kim, Seon-Kyu (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.4, no.2, 2002 , pp. 67-70 More about this Journal
Abstract
The RAPD data were used to assess genetic similarity among f grape cultivars. Of the 100 random primers tested on genomic DNA, 10 primers could be selected for Benetic analysis, and the selected primers generated a total of 115 distinct amplification fragments. A similarity matrix was constructed on the basis of the presence or absence of bands. The 7 grape cultivars analyzed with UPGMA were clustered into two groups of A and B. The similarity coefficient value of cultivars was high. The mean similarity index for all pairwise comparisons was 0.851, and ranged from 0.714 ('Rosaki' and 'Black Olympia') to 0.988 ('Kyoho' and 'Daebong'). After due consideration of differences in cultural and morphological characteristics of these two theoretically identical cultivars, it could be deduced that 'Daebong' is a bud sport of 'Kyoho' cultivar.
Keywords
Genetic similarity; random primer; UPGMA; genetic analysis; similarity matrix;
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