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http://dx.doi.org/10.3807/JOSK.2013.17.1.091

Optical Monitoring of Tumors in BALB/c Nude Mice Using Optical Coherence Tomography  

Song, Hyun-Woo (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Lee, Sang-Won (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Jung, Myung-Hwan (Proton Engineering Frontier Project, Korea Atomic Energy Research Institute)
Kim, Kye Ryung (Proton Engineering Frontier Project, Korea Atomic Energy Research Institute)
Yang, Seungkyoung (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Park, Jeong Won (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Jeong, Min-Sook (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Jung, Moon Youn (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Kim, Seunghwan (Bio-health IT Convergence Research Department, Electronics and Telecommunications Research Institute)
Publication Information
Journal of the Optical Society of Korea / v.17, no.1, 2013 , pp. 91-96 More about this Journal
Abstract
We report a method for optical monitoring of tumors in an animal model using optical coherence tomography (OCT). In a spectral domain OCT system, a superluminescent diode light source with a full width of 66 nm at half maximum and peak wavelength of 950 nm was used to take images having an axial resolution of 6.8 ${\mu}m$. Cancer cells of PC-3 were cultured and inoculated into the hypodermis of auricle tissues in BALB/c nude mice. We observed tumor formation and growth at the injection region of cancer cells in vivo and obtained the images of tumor mass center and sparse circumferences. On the $5^{th}$ day from an inoculation of cancer cells, histological images of the tumor region using cross-sectional slicing and dye staining of specimens were taken in order to confirm the correlation with the high resolution OCT images. The OCT image of tumor mass compared with normal tissues was analyzed using its A-scan data so as to obtain a tissue attenuation rate which increases according to tumor growth.
Keywords
Optical coherence tomography; Tumor; Tissue attenuation; Mouse model;
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