Browse > Article
http://dx.doi.org/10.3839/jabc.2022.043

Combinatorial effects of arginine and n-hexane extract from Korean red ginseng marc against Streptococcus mutans  

Dong Chung Kim (Department of Chemical Engineering, Chungwoon University)
Man-Jin In (Department of Chemical Engineering, Chungwoon University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 337-341 More about this Journal
Abstract
In this study, the effect of the combined use of a lipid-soluble n-hexane extract of red ginseng marc (HERGM) and water-soluble arginine, which exhibits anticaries activity, on the growth of Streptococccus mutans was investigated. As a result of checkerboard assay, HERGM and arginine showed a synergistic effect in inhibiting the growth of S. mutans with a fractional inhibitory concentration index of 0.396. Combination treatments of HERGM and arginine resulted in leakage of nucleic acid components and decrease in the viable cell counts of S. mutans, all of which were proportional to the concentration of HERGM. In conclusion, the synergistic effect of HERGM and arginine on the growth inhibition of S. mutans is mainly attributed to HERGM.
Keywords
Arginine; Growth inhibition; n-hexane extract of red ginseng marc; Streptococcus mutans;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Lee HJ, Jang HD, Lee KW, Lee HJ, Kang NJ, Kim SY, Yang H (2019) Functional food. Soohaksa, Seoul
2 Loesche WJ (1986) Role of Streptococcus mutans in human dental decay. Microbiol Rev 50: 353-380   DOI
3 Rabin N, Zheng Y, Opoku-Temeng, C, Du Y, Bonsu E, Sintim HO (2015) Biofilm formation mechanism and targets for developing antibiofilm agents. Future Med Chem 7: 493-512. doi: 10.4155/fmc.15.6   DOI
4 Bowen WH, Koo H (2011) Biology of Streptococcus mutans-derived glucosyltransferase: Role in extracellular matrix formation of cariogenic biofilms. Caries Res 45: 69-86. doi: 10.1159/000324598   DOI
5 Moon KH, Lee YC, Kim JN (2019) Effects of foreign plant extracts on cell growth and biofilm formation of Streptococcus mutans. J Life Sci 29: 712-723. doi: 10.5352/JLS.2019.29.6.712   DOI
6 Cho SJ (2021) New approaches to the control of pathogenic oral bacteria. J Life Sci 31: 100-108. doi: 10.5352/JLS.2021.31.1.100   DOI
7 Burne RA, Marquis RE (2000) Alkali production by oral bacteria and protection against dental caries. FEMS Mirobiol Lett 193: 1-6. doi: 10.1111/j.1574-6968.2000.tb09393.x   DOI
8 Burne RA, Chakraborty B (2017) Effects of arginine on Streptococcus mutans growth, virulence gene expression, and stress tolerance. Appl Environ Microbiol 83: e00496-17. doi: 10.1128/AEM.00496-17   DOI
9 He J, Hwang G, Liu Y, Gao L, Kilpatrick-Liverman L, Santarpia P, Zhou X, Koo H (2016) L-Arginine modified the exopolysaccharide matrix and thwarts Streptococcus mutans outgrowth within mixed-species oral biofilm. J Bacteriol 198: 2651-2661. doi: 10.1128/JB.00021-16   DOI
10 Huang X, Zhang K, Deng M, Exterkate RAM, Liu C, Zhou X, Cheng L, Ten Cate JM (2017) Effect of arginine on the growth and biofilm formation of oral bacteria. Arch Oral Biol 82: 256-262. doi: 10.1016/j.archoralbio.2017.06.026   DOI
11 Liu Y, Liu S, Zhi Q, Zhuang P, Zhang R, Zhang Z, Zhang K, Sun Y (2022) Arginine-induced metabolomics perturbation in Streptococcus mutans. J Oral Microbiol 14: 2015166. doi: 10.1080/20002297/2021.2015166   DOI
12 Zheng X, Cheng X, Wang L, Qiu W, Wang S, Zhou Y, Li M, Li Y, Cheng L, Li J, Zhou X, Xu X (2015) Combinatorial effects of arginine and fluoride on oral bacteria. J Dent Res 94: 344-353. doi: 10.1177/0022034514561259   DOI
13 Kim DC, In MJ (2021) Inhibitory effect of n-hexane extract from Korean red ginseng marc against Streptococcus mutans causing dental caries. J Appl Biol Chem 64: 357-362. doi: 10.3839/jabc.2021.048   DOI
14 Bijle MNA, Ekambaram M, Lo ECM, Yiu CKY (2019) The combined antimicrobial effect of arginine and fluoride toothpaste. Sci Rep 9: 8405. doi: 10.1038/s41598-019-44612-6   DOI
15 Attele AS, Wu JA, Yuan CS (1999) Ginseng pharmacology: Multiple constituents and multiple actions. Biochem Pharmacol 58: 1685-1693. doi: 10.1016/S0006-2952(99)00212-9   DOI
16 Son HJ, Han MS, Ryu GH (2009) Antibacterial activities of Et-OH extract from extruded white ginseng on tooth decay bacteria. J Korean Soc Food Sci Nutr 38: 951-957. doi: 10.3746/jkfn.2009.38.7.951   DOI
17 Sopirala MM, Mangino JE, Gebreyes WA, Biller B, Bannerman T, Balada-Llasat JM, Pancholi P (2010) Synergy testing by etest, microdilution checkerboard, and time-kill methods for pan-drug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 54: 4678-4683. doi: 10.1128/AAC.00497-10   DOI
18 Xue P, Yao Y, Yang XS, Feng J, Ren GX (2017) Improved antimicrobial effect of ginseng extract by heat transformation. J Ginseng Res 41: 180-187. doi: 10.1016/j.jgr.2016.03.002   DOI
19 Toda A, Nakayama-Imaohji H, Yamasaki H, Hasibul K, Yoneda S, Uchida K, Nariya H, Suzuki M, Miyake M, Kuwahara T (2016) Cleaning effect of acidic L-arginine on human oral biofilm. BMC Oral Health 16: 40. doi: 10.1186/s12903-016-0194-z   DOI
20 In MJ, Chae HJ, Kim DC (2014) In vitro antioxidant and anticancer potential of n-hexane extract from ginseng marc. J Appl Biol Chem 57: 247-250. doi: 10.3839/jabc.2014.039   DOI
21 Xue P, Yao Y, Yang XS, Feng J, Ren GX (2017) Improved antimicrobial effect of ginseng extract by heat transformation. J Ginseng Res 41: 180-187. doi: 10.1016/jgr.2016.03.002   DOI
22 Nascimento MM, Alvarez AJ, Huang X, Browngardt C, Jenkins R, Sinhoreti MC, Ribeiro APD, Dilbone DA, Richards VP, Garrett TJ, Burne RA (2019) Metabolic profile of supragingival plaque exposed to arginine and fluoride. J Dent Res 98: 1245-1252. doi: 10.1177/0022034519869906   DOI
23 Bijle MN, Neelakantan P, Ekambaram M, Lo ECM, Yiu CKY (2020) effect of a novel symbiotic on Streptococcus mutans. Sci Rep 10: 7951. doi: 10.1038/s41598-020-64956-8   DOI
24 Kim HS, Lee SW, Sydara K, Cho SJ (2019) Antibacterial and antibiofilm activities of Diospyros malabarica stem extract against Streptococcus mutans. J Life Sci 29: 90-96. doi: 10.5352/JLS.2019.29.1.90   DOI
25 Li CM, Yu JP (2014) Chemical composition, antimicrobial activity and mechanism of action of essential oil from the leaves of Macleaya cordata (Willd.) R. Br. J Food Safety 35: 227-236. doi: 10.1111/jfs.12175   DOI
26 Cha SM, Han SB, Lee YS, Cha JD (2015) Synergistic effect of the ethanol extract of Alismatis rhizome against oral pathogens. J Oral Biol 2: 7. doi: 10.13188/2377-987x.1000007   DOI
27 Lee DS, Song HG (2020) Interactions between antimicrobial substances from bacteria against various microorganisms. Korean J Microbiol 56: 214-221. doi: 10. 7845/kjm.2020.0063   DOI