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http://dx.doi.org/10.3839/jabc.2021.049

Flavone from the Lycopersicon esculentum and their antioxidant capacity through GSH recovery effect  

Jeon, Hyeong-Ju (Department of Food & Nutrition, Jangan University)
Kim, Hyoung-Geun (Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.4, 2021 , pp. 363-368 More about this Journal
Abstract
The fruits of tomato (Lycopersicon esculentum) were extracted with 70% aqueous methanol (MeOH) and the concentrates were partitioned into ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H2O) fractions. The repeated silica gel (SiO2) and octadecyl silica gel column chromatographies for the EtOAc fraction, whose activity was confirmed, led to isolation of one flavone compound. Nuclear magnetic resornance, infrarad spectroscopy, and mass spectroscopy (MS) revealed the chemical structure of the isolated compound, 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone (1). LC-MS/MS analysis determined the content level of compounds 1 in the MeOH extract to be 4.02±0.12 ㎍/mg and in the TME-10 fraction to be 0.96±0.03 ㎍/mg. Through this study, the antioxidantive capacity was confirmed by demonstrating that the L. esculentum extract and their fractions showing an increase in glutathione mean and a decrease in glutathione heterogeneity uniformly raises the intracellular glutathione level.
Keywords
Flavone; Glutathione recovery; Lycopersicon esculentum; Nuclear magnetic resornance;
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1 Aneta G, Malgorzata MD (2014) Lycopene-antioxidant with radioprotective and anticancer properties. A review. Rocz Panstw Zakl Hig 65(4): 263-271
2 Jung EB, Shin MK (1990) In Hyang Yak DaeSaJun. 3rd edition. Young Lim Sa, Seoul
3 Wu G, Fang YZ, Yang S, Lupton RJ, Turner DN (2004) Glutathione metabolism and its implications for health. J Nutr 134(3): 489-492. doi: 10.1093/jn/134.3.489   DOI
4 Mailloux RJ, McBride SL, Harper ME (2013) Unearthing the secrets of mitochondrial ROS and glutathione in bioenergetics. Trends Biochem Sci 38(12): 592-602. doi: 10.1016/j.tibs.2013.09.001   DOI
5 Yang SL, Yu PL, Chung KR (2016) The glutathione peroxidase-mediated reactive oxygen species resistance, fungicide sensitivity and cell wall construction in the citrus fungal pathogen Alternaria alternata. Environ Microbiol 18(3): 923-935. doi: 10.1111/1462-2920.13125   DOI
6 Kim HG, Jung YS, Oh SM, Oh HJ, Ko JH, Kim DO, Kang SC, Lee YG, Lee DY, Baek NI (2020) Coreolanceolins A-E, New Flavanones from the Flowers of Coreopsis lanceolata and Their Antioxidative and Antiinflammatory Effects. Antioxidants 9: 539-555. doi: 10.3390/antiox9060539   DOI
7 Kim HG, Jeon HJ (2020) Antioxidant compound from the Lycopersicon esculentum. J Appl Biol Chem 63(4): 319-325. doi: 10.3839/jabc.2020.042   DOI
8 Maleki SJ, Crespo JF, Cabanillas B (2019) Anti-inflammatory effects of flavonoids. Food Chem 299: 125124. doi : 10.1016/j.foodchem.2019.125124   DOI
9 Ko JH, Nam YH, Joo SW, Kim HG, Lee YG, Kang TH, Baek NI (2018) Flavonoid 8-O-Glucuronides from the Aerial Parts of Malva verticillata and Their Recovery Effects on Alloxan-Induced Pancreatic Islets in Zebrafish. Molecules 23:833-847. doi: 10.3390/molecules23040833   DOI